prikaz prve stranice dokumenta Francisella novicida-Containing Vacuole within Dictyostelium discoideum: Isolation and Proteomic Characterization
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Francisella novicida-Containing Vacuole within Dictyostelium discoideum: Isolation and Proteomic Characterization
Microorganisms, 12 (2024), 10; 1949. https://doi.org/10.3390/microorganisms12101949
Marečić, Valentina; Shevchuk, Olga; Link, Marek; Viduka, Ina; Ožanić, Mateja; Kostanjšek, Rok; Mihelčić, Mirna; Antonić, Maša; Jänsch, Lothar; Stulik, Jiri; Šantic, Marina

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Marečić, V., Shevchuk, O., Link, M., Viduka, I., Ožanić, M., Kostanjšek, R. ... Šantic, M. (2024). Francisella novicida-Containing Vacuole within Dictyostelium discoideum: Isolation and Proteomic Characterization. Microorganisms, 12. (10). doi: 10.3390/microorganisms12101949

Marečić, Valentina, et al. "Francisella novicida-Containing Vacuole within Dictyostelium discoideum: Isolation and Proteomic Characterization." Microorganisms, vol. 12, br. 10, 2024. https://doi.org/10.3390/microorganisms12101949

Marečić, Valentina, Olga Shevchuk, Marek Link, Ina Viduka, Mateja Ožanić, Rok Kostanjšek, Mirna Mihelčić, et al. "Francisella novicida-Containing Vacuole within Dictyostelium discoideum: Isolation and Proteomic Characterization." Microorganisms 12, br. 10 (2024). https://doi.org/10.3390/microorganisms12101949

Marečić, V., et al. (2024) 'Francisella novicida-Containing Vacuole within Dictyostelium discoideum: Isolation and Proteomic Characterization', Microorganisms, 12(10). doi: 10.3390/microorganisms12101949

Marečić V, Shevchuk O, Link M, Viduka I, Ožanić M, Kostanjšek R, i sur.. Francisella novicida-Containing Vacuole within Dictyostelium discoideum: Isolation and Proteomic Characterization. Microorganisms [Internet]. 26.09.2024. [pristupljeno 11.11.2024.];12(10). doi: 10.3390/microorganisms12101949

V. Marečić, et al., "Francisella novicida-Containing Vacuole within Dictyostelium discoideum: Isolation and Proteomic Characterization", Microorganisms, vol. 12, br. 10, Rujan 2024. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:090137. [Citirano: 11.11.2024.]

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Naslov (engleski)Francisella novicida-Containing Vacuole within Dictyostelium discoideum: Isolation and Proteomic Characterization
AutorValentina Marečić
AutorOlga Shevchuk
AutorMarek Link
AutorIna Viduka
AutorMateja Ožanić
AutorRok Kostanjšek
AutorMirna Mihelčić
AutorMaša Antonić
AutorLothar Jänsch
AutorJiri Stulik
AutorMarina Šantic
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za mikrobiologiju i parazitologiju)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Kliničke medicinske znanosti
Medicinska mikrobiologija
Sažetak (engleski)
Francisella is a highly infectious gram-negative bacterium that causes tularemia in humans and animals. It can survive and multiply in a variety of cells, including macrophages, dendritic cells, amoebae, and arthropod-derived cells. However, the intracellular life cycle of a bacterium varies depending on the cell type. Shortly after the infection of mammalian cells, the bacterium escapes the phagosome into the cytosol, where it replicates. In contrast, in the amoebae Acanthamoeba castellanii and Hartmannella vermiformis, the bacterium replicates within the membrane-bound vacuole. In recent years, the amoeba Dictyostelium discoideum has emerged as a powerful model to study the intracellular cycle and virulence of many pathogenic bacteria. In this study, we used D. discoideum as a model for the infection and isolation of Francisella novicida-containing vacuoles (FCVs) formed after bacteria invade the amoeba. Our results showed that F. novicida localized in a vacuole after invading D. discoideum. Here, we developed a method to isolate FCV and determined its composition by proteomic analyses. Proteomic analyses revealed 689 proteins, including 13 small GTPases of the Rab family. This is the first evidence of F. novicida-containing vacuoles within amoeba, and this approach will contribute to our understanding of host–pathogen interactions and the process of pathogen vacuole formation, as vacuoles containing bacteria represent direct contact between pathogens and their hosts. Furthermore, this method can be translocated on other amoeba models.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaMicroorganisms
Brojčani podacivol. 12, br. 10, 1949
e-ISSN2076-2607
DOIhttps://doi.org/10.3390/microorganisms12101949
URN:NBNurn:nbn:hr:184:090137
Datum objave publikacije2024-09-26
URL dokumentahttps://www.mdpi.com/2076-2607/12/10/1949
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 4.0 međunarodna (CC BY 4.0)
Datum i vrijeme pohrane2024-10-03 10:26:43