prikaz prve stranice dokumenta Francisella novicida-Containing Vacuole within Dictyostelium discoideum: Isolation and Proteomic Characterization
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Scientific paper - Original scientific paper
Francisella novicida-Containing Vacuole within Dictyostelium discoideum: Isolation and Proteomic Characterization
Microorganisms, 12 (2024), 10; 1949. https://doi.org/10.3390/microorganisms12101949
Marečić, Valentina; Shevchuk, Olga; Link, Marek; Viduka, Ina; Ožanić, Mateja; Kostanjšek, Rok; Mihelčić, Mirna; Antonić, Maša; Jänsch, Lothar; Stulik, Jiri; Šantic, Marina

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Marečić, V., Shevchuk, O., Link, M., Viduka, I., Ožanić, M., Kostanjšek, R. ... Šantic, M. (2024). Francisella novicida-Containing Vacuole within Dictyostelium discoideum: Isolation and Proteomic Characterization. Microorganisms, 12. (10). doi: 10.3390/microorganisms12101949

Marečić, Valentina, et al. "Francisella novicida-Containing Vacuole within Dictyostelium discoideum: Isolation and Proteomic Characterization." Microorganisms, vol. 12, no. 10, 2024. https://doi.org/10.3390/microorganisms12101949

Marečić, Valentina, Olga Shevchuk, Marek Link, Ina Viduka, Mateja Ožanić, Rok Kostanjšek, Mirna Mihelčić, et al. "Francisella novicida-Containing Vacuole within Dictyostelium discoideum: Isolation and Proteomic Characterization." Microorganisms 12, no. 10 (2024). https://doi.org/10.3390/microorganisms12101949

Marečić, V., et al. (2024) 'Francisella novicida-Containing Vacuole within Dictyostelium discoideum: Isolation and Proteomic Characterization', Microorganisms, 12(10). doi: 10.3390/microorganisms12101949

Marečić V, Shevchuk O, Link M, Viduka I, Ožanić M, Kostanjšek R, and sur.. Francisella novicida-Containing Vacuole within Dictyostelium discoideum: Isolation and Proteomic Characterization. Microorganisms [Internet]. 2024 September 26 [cited 2024 December 22];12(10). doi: 10.3390/microorganisms12101949

V. Marečić, et al., "Francisella novicida-Containing Vacuole within Dictyostelium discoideum: Isolation and Proteomic Characterization", Microorganisms, vol. 12, no. 10, September 2024. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:090137. [Accessed: 22 December 2024]

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Metadata
Title (english)Francisella novicida-Containing Vacuole within Dictyostelium discoideum: Isolation and Proteomic Characterization
AuthorValentina Marečić
AuthorOlga Shevchuk
AuthorMarek Link
AuthorIna Viduka
AuthorMateja Ožanić
AuthorRok Kostanjšek
AuthorMirna Mihelčić
AuthorMaša Antonić
AuthorLothar Jänsch
AuthorJiri Stulik
AuthorMarina Šantic
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Microbiology and Parasitology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Medical Microbiology
Abstract (english)
Francisella is a highly infectious gram-negative bacterium that causes tularemia in humans and animals. It can survive and multiply in a variety of cells, including macrophages, dendritic cells, amoebae, and arthropod-derived cells. However, the intracellular life cycle of a bacterium varies depending on the cell type. Shortly after the infection of mammalian cells, the bacterium escapes the phagosome into the cytosol, where it replicates. In contrast, in the amoebae Acanthamoeba castellanii and Hartmannella vermiformis, the bacterium replicates within the membrane-bound vacuole. In recent years, the amoeba Dictyostelium discoideum has emerged as a powerful model to study the intracellular cycle and virulence of many pathogenic bacteria. In this study, we used D. discoideum as a model for the infection and isolation of Francisella novicida-containing vacuoles (FCVs) formed after bacteria invade the amoeba. Our results showed that F. novicida localized in a vacuole after invading D. discoideum. Here, we developed a method to isolate FCV and determined its composition by proteomic analyses. Proteomic analyses revealed 689 proteins, including 13 small GTPases of the Rab family. This is the first evidence of F. novicida-containing vacuoles within amoeba, and this approach will contribute to our understanding of host–pathogen interactions and the process of pathogen vacuole formation, as vacuoles containing bacteria represent direct contact between pathogens and their hosts. Furthermore, this method can be translocated on other amoeba models.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleMicroorganisms
Numberingvol. 12, no. 10, 1949
e-ISSN2076-2607
DOIhttps://doi.org/10.3390/microorganisms12101949
URN:NBNurn:nbn:hr:184:090137
Publication2024-09-26
Document URLhttps://www.mdpi.com/2076-2607/12/10/1949
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2024-10-03 10:26:43