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Antigenic determinants driving serogroup-specific antibody response to Neisseria meningitidis C, W, and Y capsular polysaccharides: Insights for rational vaccine design
Carbohydrate Polymers, 341 (2024); 122349. https://doi.org/10.1016/j.carbpol.2024.122349
Pietri, Gian Pietro; Bertuzzi, Sara; Karničar, Katarina; Unione, Luca; Lisnić, Berislav; Malić, Suzana; Miklić, Karmela; Novak, Matej; Calloni, Ilaria; Santini, Laura; Usenik, Aleksandra; Romano, Maria Rosaria; Adamo, Roberto; Jonjić, Stipan; Turk, Dušan; Jiménez-Barbero, Jesús; Lenac Roviš, Tihana Više autora...

Citirajte ovaj rad

Pietri, G. P., Bertuzzi, S., Karničar, K., Unione, L., Lisnić, B., Malić, S. ... Lenac Roviš, T. (2024). Antigenic determinants driving serogroup-specific antibody response to Neisseria meningitidis C, W, and Y capsular polysaccharides: Insights for rational vaccine design. Carbohydrate Polymers, 341.. doi: 10.1016/j.carbpol.2024.122349

Pietri, Gian Pietro, et al. "Antigenic determinants driving serogroup-specific antibody response to Neisseria meningitidis C, W, and Y capsular polysaccharides: Insights for rational vaccine design." Carbohydrate Polymers, vol. 341, 2024. https://doi.org/10.1016/j.carbpol.2024.122349

Pietri, Gian Pietro, Sara Bertuzzi, Katarina Karničar, Luca Unione, Berislav Lisnić, Suzana Malić, Karmela Miklić, et al. "Antigenic determinants driving serogroup-specific antibody response to Neisseria meningitidis C, W, and Y capsular polysaccharides: Insights for rational vaccine design." Carbohydrate Polymers 341 (2024). https://doi.org/10.1016/j.carbpol.2024.122349

Pietri, G. P., et al. (2024) 'Antigenic determinants driving serogroup-specific antibody response to Neisseria meningitidis C, W, and Y capsular polysaccharides: Insights for rational vaccine design', Carbohydrate Polymers, 341. doi: 10.1016/j.carbpol.2024.122349

Pietri GP, Bertuzzi S, Karničar K, Unione L, Lisnić B, Malić S, i sur.. Antigenic determinants driving serogroup-specific antibody response to Neisseria meningitidis C, W, and Y capsular polysaccharides: Insights for rational vaccine design. Carbohydrate Polymers [Internet]. 31.05.2024. [pristupljeno 01.10.2024.];341. doi: 10.1016/j.carbpol.2024.122349

G. P. Pietri, et al., "Antigenic determinants driving serogroup-specific antibody response to Neisseria meningitidis C, W, and Y capsular polysaccharides: Insights for rational vaccine design", Carbohydrate Polymers, vol. 341, Svibanj 2024. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:145755. [Citirano: 01.10.2024.]

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Podaci o radu
Naslov (engleski)Antigenic determinants driving serogroup-specific antibody response to Neisseria meningitidis C, W, and Y capsular polysaccharides: Insights for rational vaccine design
AutorGian Pietro Pietri
AutorSara Bertuzzi
AutorKatarina Karničar
AutorLuca Unione
AutorBerislav Lisnić
AutorSuzana Malić
AutorKarmela Miklić
AutorMatej Novak
AutorIlaria Calloni
AutorLaura Santini
AutorAleksandra Usenik
AutorMaria Rosaria Romano
AutorRoberto Adamo
AutorStipan Jonjić
AutorDušan Turk
AutorJesús Jiménez-Barbero
AutorTihana Lenac Roviš
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Centar za proteomiku)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Sažetak (engleski)
Meningococcal glycoconjugate vaccines sourced from capsular polysaccharides (CPSs) of pathogenic Neisseria meningitidis strains are well-established measures to prevent meningococcal disease. However, the exact structural factors responsible for antibody recognition are not known. CPSs of Neisseria meningitidis serogroups Y and W differ by a single stereochemical center, yet they evoke specific immune responses. Herein, we developed specific monoclonal antibodies (mAbs) targeting serogroups C, Y, and W and evaluated their ability to kill bacteria. We then used these mAbs to dissect structural elements responsible for carbohydrate-protein interactions. First, Men oligosaccharides were screened against the mAbs using ELISA to select putative lengths representing the minimal antigenic determinant. Next, molecular interaction features between the mAbs and serogroup-specific sugar fragments were elucidated using STD-NMR. Moreover, X-ray diffraction data with the anti-MenW CPS mAb enabled the elucidation of the sugar-antibody binding mode. Our findings revealed common traits in the epitopes of all three sialylated serogroups. The minimal binding epitopes typically comprise five to six repeating units. Moreover, the O-acetylation of the neuraminic acid moieties was fundamental for mAb binding. These insights hold promise for the rational design of optimized meningococcal oligosaccharides, opening new avenues for novel production methods, including chemical or enzymatic approaches.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaCarbohydrate Polymers
Brojčani podacivol. 341, 122349
p-ISSN0144-8617
DOIhttps://doi.org/10.1016/j.carbpol.2024.122349
URN:NBNurn:nbn:hr:184:145755
Datum objave elektroničkog izdanja2024-05-31
ProjektŠifra: KK.01.1.1.01.0006
Naziv (hrvatski): Jačanje kapaciteta CERVIRVAC-a za istraživanja u virusnoj imunologiji i vakcinologiji
Naziv (engleski): Strengthening the capacity of CerVirVac (Croatian Centre of Excellence for Virus Immunology and Vaccines) for research in viral immunology and vaccinology
Voditelj: Stipan Jonjić
Pravna nadležnost: eu
Financijer: Europska komisija
Linija financiranja: European Regional Development Fund
ProjektŠifra: N/A
Naziv (hrvatski): Centar izvrsnosti za virusnu imunologiju i cjepiva
Naziv (engleski): Centre of Excellence for Research in Viral Immunology and the Development of New Vaccines
Kratica: CERVirVac
Voditelj: Stipan Jonjić
Pravna nadležnost: Hrvatska
Financijer: Ministarstvo znanosti i obrazovanja Republike Hrvatske
Linija financiranja: Centres of Research Excellence
URL dokumentahttps://linkinghub.elsevier.com/retrieve/pii/S0144861724005757
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje-Nekomercijalno-Bez prerada 4.0 međunarodna (CC BY-NC-ND 4.0)
Datum i vrijeme pohrane2024-06-10 06:48:43