prikaz prve stranice dokumenta Antigenic determinants driving serogroup-specific antibody response to Neisseria meningitidis C, W, and Y capsular polysaccharides: Insights for rational vaccine design
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Scientific paper - Original scientific paper
Antigenic determinants driving serogroup-specific antibody response to Neisseria meningitidis C, W, and Y capsular polysaccharides: Insights for rational vaccine design
Carbohydrate Polymers, 341 (2024); 122349. https://doi.org/10.1016/j.carbpol.2024.122349
Pietri, Gian Pietro; Bertuzzi, Sara; Karničar, Katarina; Unione, Luca; Lisnić, Berislav; Malić, Suzana; Miklić, Karmela; Novak, Matej; Calloni, Ilaria; Santini, Laura; Usenik, Aleksandra; Romano, Maria Rosaria; Adamo, Roberto; Jonjić, Stipan; Turk, Dušan; Jiménez-Barbero, Jesús; Lenac Roviš, Tihana More authors...

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Pietri, G. P., Bertuzzi, S., Karničar, K., Unione, L., Lisnić, B., Malić, S. ... Lenac Roviš, T. (2024). Antigenic determinants driving serogroup-specific antibody response to Neisseria meningitidis C, W, and Y capsular polysaccharides: Insights for rational vaccine design. Carbohydrate Polymers, 341.. doi: 10.1016/j.carbpol.2024.122349

Pietri, Gian Pietro, et al. "Antigenic determinants driving serogroup-specific antibody response to Neisseria meningitidis C, W, and Y capsular polysaccharides: Insights for rational vaccine design." Carbohydrate Polymers, vol. 341, 2024. https://doi.org/10.1016/j.carbpol.2024.122349

Pietri, Gian Pietro, Sara Bertuzzi, Katarina Karničar, Luca Unione, Berislav Lisnić, Suzana Malić, Karmela Miklić, et al. "Antigenic determinants driving serogroup-specific antibody response to Neisseria meningitidis C, W, and Y capsular polysaccharides: Insights for rational vaccine design." Carbohydrate Polymers 341 (2024). https://doi.org/10.1016/j.carbpol.2024.122349

Pietri, G. P., et al. (2024) 'Antigenic determinants driving serogroup-specific antibody response to Neisseria meningitidis C, W, and Y capsular polysaccharides: Insights for rational vaccine design', Carbohydrate Polymers, 341. doi: 10.1016/j.carbpol.2024.122349

Pietri GP, Bertuzzi S, Karničar K, Unione L, Lisnić B, Malić S, and sur.. Antigenic determinants driving serogroup-specific antibody response to Neisseria meningitidis C, W, and Y capsular polysaccharides: Insights for rational vaccine design. Carbohydrate Polymers [Internet]. 2024 May 31 [cited 2024 November 14];341. doi: 10.1016/j.carbpol.2024.122349

G. P. Pietri, et al., "Antigenic determinants driving serogroup-specific antibody response to Neisseria meningitidis C, W, and Y capsular polysaccharides: Insights for rational vaccine design", Carbohydrate Polymers, vol. 341, May 2024. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:145755. [Accessed: 14 November 2024]

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Metadata
Title (english)Antigenic determinants driving serogroup-specific antibody response to Neisseria meningitidis C, W, and Y capsular polysaccharides: Insights for rational vaccine design
AuthorGian Pietro Pietri
AuthorSara Bertuzzi
AuthorKatarina Karničar
AuthorLuca Unione
AuthorBerislav Lisnić
AuthorSuzana Malić
AuthorKarmela Miklić
AuthorMatej Novak
AuthorIlaria Calloni
AuthorLaura Santini
AuthorAleksandra Usenik
AuthorMaria Rosaria Romano
AuthorRoberto Adamo
AuthorStipan Jonjić
AuthorDušan Turk
AuthorJesús Jiménez-Barbero
AuthorTihana Lenac Roviš
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Center for Proteomics)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
Meningococcal glycoconjugate vaccines sourced from capsular polysaccharides (CPSs) of pathogenic Neisseria meningitidis strains are well-established measures to prevent meningococcal disease. However, the exact structural factors responsible for antibody recognition are not known. CPSs of Neisseria meningitidis serogroups Y and W differ by a single stereochemical center, yet they evoke specific immune responses. Herein, we developed specific monoclonal antibodies (mAbs) targeting serogroups C, Y, and W and evaluated their ability to kill bacteria. We then used these mAbs to dissect structural elements responsible for carbohydrate-protein interactions. First, Men oligosaccharides were screened against the mAbs using ELISA to select putative lengths representing the minimal antigenic determinant. Next, molecular interaction features between the mAbs and serogroup-specific sugar fragments were elucidated using STD-NMR. Moreover, X-ray diffraction data with the anti-MenW CPS mAb enabled the elucidation of the sugar-antibody binding mode. Our findings revealed common traits in the epitopes of all three sialylated serogroups. The minimal binding epitopes typically comprise five to six repeating units. Moreover, the O-acetylation of the neuraminic acid moieties was fundamental for mAb binding. These insights hold promise for the rational design of optimized meningococcal oligosaccharides, opening new avenues for novel production methods, including chemical or enzymatic approaches.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleCarbohydrate Polymers
Numberingvol. 341, 122349
p-ISSN0144-8617
DOIhttps://doi.org/10.1016/j.carbpol.2024.122349
URN:NBNurn:nbn:hr:184:145755
Online first2024-05-31
ProjectNumber: KK.01.1.1.01.0006
Title (croatian): Jačanje kapaciteta CERVIRVAC-a za istraživanja u virusnoj imunologiji i vakcinologiji
Title (english): Strengthening the capacity of CerVirVac (Croatian Centre of Excellence for Virus Immunology and Vaccines) for research in viral immunology and vaccinology
Leader: Stipan Jonjić
Jurisdiction: eu
Funding stream: European Regional Development Fund
ProjectNumber: N/A
Title (croatian): Centar izvrsnosti za virusnu imunologiju i cjepiva
Title (english): Centre of Excellence for Research in Viral Immunology and the Development of New Vaccines
Acronym: CERVirVac
Leader: Stipan Jonjić
Jurisdiction: Croatia
Funding stream: Centres of Research Excellence
Document URLhttps://linkinghub.elsevier.com/retrieve/pii/S0144861724005757
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) icon
Created on2024-06-10 06:48:43