prikaz prve stranice dokumenta Arf1 and Arf6 GTPases are required for intracellular transport of cytomegalovirus virions
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Izlaganje na skupu
Arf1 and Arf6 GTPases are required for intracellular transport of cytomegalovirus virions
Na: 3rd Regional Congress of Physiological Societies and 5th Congress of Croatian Physiological Society. Plitvice, Hrvatska, 2022. urn:nbn:hr:184:888131
Pavišić, Valentino; Mahmutefendić Lučin, Hana; Lukanović Jurić, Silvija; Lučin, Pero; Blagojević Zagorac, Gordana

Citirajte ovaj rad

Pavišić, V., Mahmutefendić Lučin, H., Lukanović Jurić, S., Lučin, P. i Blagojević Zagorac, G. Arf1 and Arf6 GTPases are required for intracellular transport of cytomegalovirus virions [PowerPoint prezentacija]. Preuzeto s https://urn.nsk.hr/urn:nbn:hr:184:888131

Pavišić, Valentino, et al. "Arf1 and Arf6 GTPases are required for intracellular transport of cytomegalovirus virions." 3rd Regional Congress of Physiological Societies and 5th Congress of Croatian Physiological Society, 22.09.2022.-24.09.2022., Plitvice, Hrvatska. Prezentacija. https://urn.nsk.hr/urn:nbn:hr:184:888131

Pavišić, Valentino, Hana Mahmutefendić Lučin, Silvija Lukanović Jurić, Pero Lučin i Gordana Blagojević Zagorac. "Arf1 and Arf6 GTPases are required for intracellular transport of cytomegalovirus virions." Prezentacija sa skupa 3rd Regional Congress of Physiological Societies and 5th Congress of Croatian Physiological Society, Plitvice, Hrvatska, 22.09.2022.-24.09.2022. https://urn.nsk.hr/urn:nbn:hr:184:888131

Pavišić, V., et al. 'Arf1 and Arf6 GTPases are required for intracellular transport of cytomegalovirus virions' [Prezentacija], 3rd Regional Congress of Physiological Societies and 5th Congress of Croatian Physiological Society. 22.09.2022.-24.09.2022. Preuzeto s: https://urn.nsk.hr/urn:nbn:hr:184:888131

Pavišić V, Mahmutefendić Lučin H, Lukanović Jurić S, Lučin P, Blagojević Zagorac G. Arf1 and Arf6 GTPases are required for intracellular transport of cytomegalovirus virions. [Prezentacija] [pristupljeno 15.11.2024.] Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:888131

V. Pavišić, H. Mahmutefendić Lučin, S. Lukanović Jurić, P. Lučin i G. Blagojević Zagorac, "Arf1 and Arf6 GTPases are required for intracellular transport of cytomegalovirus virions", prezentirano na: 3rd Regional Congress of Physiological Societies and 5th Congress of Croatian Physiological Society, Plitvice, 22.09.2022.-24.09.2022. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:888131

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Podaci o radu
Naslov (engleski)Arf1 and Arf6 GTPases are required for intracellular transport of cytomegalovirus virions
AutorValentino Pavišić
AutorHana Mahmutefendić Lučin
AutorSilvija Lukanović Jurić
AutorPero Lučin
AutorGordana Blagojević Zagorac
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za fiziologiju, imunologiju i patofiziologiju)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Sažetak (engleski)
Introduction Small GTPases of the Arf family are one of the key regulators of endosomal intracellular trafficking and endocytosis. We have previously shown that in cells in which Arf1 or Arf6 protein expression is knocked down by small siRNAs, the establishment of MCMV infection is impaired. Aims Because our previous results suggest that the GTPases Arf1 and Arf6 are required at the earliest stages of MCMV infection before the MCMV genome enters the nucleus, this study aimed to determine whether they are required for internalization or intracellular transport of CMV virions. Materials and methods Untransfected Balb 3T3 cells or cells transfected with control siRNA or siRNA targeting Arf proteins were infected with recombinant murine cytomegalovirus Δm138-MCMV, and the kinetics of virion internalization was followed by viral m55 envelope protein staining and flow cytometry, whereas intracellular transport was followed by viral m55 envelope protein and viral m25 tegument protein staining and confocal microscopy. Results After 30 minutes, approximately 50% of the virions initially bound to the plasma membrane were internalized in untransfected cells, and the kinetics of internalization was not impaired in cells transfected with siRNA targeting Arf proteins. After 60 minutes of internalization and especially after 240 minutes, the viral m25 tegument protein was detected in the nucleus of untransfected or cells transfected with scramble siRNA, whereas in cells transfected with siArf1 or siArf6, m25 was detected predominantly in the cytoplasm. Conclusion Our results suggest that Arf1 and Arf6 are not required for internalization of CMV virions but are required for their intracellular transport to the nucleus.
Jezikengleski
Vrsta recenzijeNepoznato
URN:NBNurn:nbn:hr:184:888131
Datum izlaganja2022-09-22
ProjektŠifra: IP-2020-02-1323
Naziv (hrvatski): Stanični sustav Arf GTPaza u patogenezi citomegalovirusne infekcije
Voditelj: Gordana Blagojević Zagorac
Pravna nadležnost: Hrvatska
Financijer: HRZZ
ProjektŠifra: IP-2019-04-3582
Naziv (hrvatski): Sekundarno omatanje i izlazak beta-herpesvirusa iz stanice
Voditelj: Pero Lučin
Pravna nadležnost: Hrvatska
Financijer: HRZZ
SkupNaziv: 3rd Regional Congress of Physiological Societies and 5th Congress of Croatian Physiological Society
Lokacija: Plitvice, Hrvatska
Datum početka: 2022-09-22
Datum završetka: 2022-09-24
Vrsta izlaganja: Predavanje
Vrsta resursaTekst
Prava pristupaOgraničen pristup
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Datum i vrijeme pohrane2023-04-05 12:06:17