prikaz prve stranice dokumenta Arf1 and Arf6 GTPases are required for intracellular transport of cytomegalovirus virions
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Conference presentation
Arf1 and Arf6 GTPases are required for intracellular transport of cytomegalovirus virions
At: 3rd Regional Congress of Physiological Societies and 5th Congress of Croatian Physiological Society. Plitvice, Croatia, 2022. urn:nbn:hr:184:888131
Pavišić, Valentino; Mahmutefendić Lučin, Hana; Lukanović Jurić, Silvija; Lučin, Pero; Blagojević Zagorac, Gordana

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Pavišić, V., Mahmutefendić Lučin, H., Lukanović Jurić, S., Lučin, P. & Blagojević Zagorac, G. Arf1 and Arf6 GTPases are required for intracellular transport of cytomegalovirus virions [PowerPoint slides]. Retrieved from https://urn.nsk.hr/urn:nbn:hr:184:888131

Pavišić, Valentino, et al. "Arf1 and Arf6 GTPases are required for intracellular transport of cytomegalovirus virions." 3rd Regional Congress of Physiological Societies and 5th Congress of Croatian Physiological Society, 22.09.2022.-24.09.2022., Plitvice, Croatia. Presentation. https://urn.nsk.hr/urn:nbn:hr:184:888131

Pavišić, Valentino, Hana Mahmutefendić Lučin, Silvija Lukanović Jurić, Pero Lučin and Gordana Blagojević Zagorac. "Arf1 and Arf6 GTPases are required for intracellular transport of cytomegalovirus virions." Presentation at 3rd Regional Congress of Physiological Societies and 5th Congress of Croatian Physiological Society, Plitvice, Croatia, 22.09.2022.-24.09.2022. https://urn.nsk.hr/urn:nbn:hr:184:888131

Pavišić, V., et al. 'Arf1 and Arf6 GTPases are required for intracellular transport of cytomegalovirus virions' [Presentation], 3rd Regional Congress of Physiological Societies and 5th Congress of Croatian Physiological Society. 22.09.2022.-24.09.2022. Available at: https://urn.nsk.hr/urn:nbn:hr:184:888131

Pavišić V, Mahmutefendić Lučin H, Lukanović Jurić S, Lučin P, Blagojević Zagorac G. Arf1 and Arf6 GTPases are required for intracellular transport of cytomegalovirus virions. [Presentation] [cited 2024 November 14] Available at: https://urn.nsk.hr/urn:nbn:hr:184:888131

V. Pavišić, H. Mahmutefendić Lučin, S. Lukanović Jurić, P. Lučin and G. Blagojević Zagorac, "Arf1 and Arf6 GTPases are required for intracellular transport of cytomegalovirus virions", presented at: 3rd Regional Congress of Physiological Societies and 5th Congress of Croatian Physiological Society, Plitvice, 22.09.2022.-24.09.2022. Available at: https://urn.nsk.hr/urn:nbn:hr:184:888131

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Metadata
Title (english)Arf1 and Arf6 GTPases are required for intracellular transport of cytomegalovirus virions
AuthorValentino Pavišić
AuthorHana Mahmutefendić Lučin
AuthorSilvija Lukanović Jurić
AuthorPero Lučin
AuthorGordana Blagojević Zagorac
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Physiology, Immunology and Pathophxsiology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
Introduction Small GTPases of the Arf family are one of the key regulators of endosomal intracellular trafficking and endocytosis. We have previously shown that in cells in which Arf1 or Arf6 protein expression is knocked down by small siRNAs, the establishment of MCMV infection is impaired. Aims Because our previous results suggest that the GTPases Arf1 and Arf6 are required at the earliest stages of MCMV infection before the MCMV genome enters the nucleus, this study aimed to determine whether they are required for internalization or intracellular transport of CMV virions. Materials and methods Untransfected Balb 3T3 cells or cells transfected with control siRNA or siRNA targeting Arf proteins were infected with recombinant murine cytomegalovirus Δm138-MCMV, and the kinetics of virion internalization was followed by viral m55 envelope protein staining and flow cytometry, whereas intracellular transport was followed by viral m55 envelope protein and viral m25 tegument protein staining and confocal microscopy. Results After 30 minutes, approximately 50% of the virions initially bound to the plasma membrane were internalized in untransfected cells, and the kinetics of internalization was not impaired in cells transfected with siRNA targeting Arf proteins. After 60 minutes of internalization and especially after 240 minutes, the viral m25 tegument protein was detected in the nucleus of untransfected or cells transfected with scramble siRNA, whereas in cells transfected with siArf1 or siArf6, m25 was detected predominantly in the cytoplasm. Conclusion Our results suggest that Arf1 and Arf6 are not required for internalization of CMV virions but are required for their intracellular transport to the nucleus.
Languageenglish
Peer reviewUnknown
URN:NBNurn:nbn:hr:184:888131
Presentation date2022-09-22
ProjectNumber: IP-2020-02-1323
Title (croatian): Stanični sustav Arf GTPaza u patogenezi citomegalovirusne infekcije
Leader: Gordana Blagojević Zagorac
Jurisdiction: Croatia
ProjectNumber: IP-2019-04-3582
Title (croatian): Sekundarno omatanje i izlazak beta-herpesvirusa iz stanice
Leader: Pero Lučin
Jurisdiction: Croatia
ConferenceTitle: 3rd Regional Congress of Physiological Societies and 5th Congress of Croatian Physiological Society
Location: Plitvice, Croatia
Start date: 2022-09-22
End date: 2022-09-24
Assembly type: Lecture
Type of resourceText
Access conditionsRestricted access
Terms of useLicence In copyright icon
Created on2023-04-05 12:06:17