prikaz prve stranice dokumenta Interaction of spin-labeled HPMA-based nanoparticles with human blood plasma proteins – the introduction of protein-corona-free polymer nanomedicine
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Interaction of spin-labeled HPMA-based nanoparticles with human blood plasma proteins – the introduction of protein-corona-free polymer nanomedicine
Nanoscale, 10 (2018), 13; 6194-6204. https://doi.org/10.1039/c7nr09355a
Klepac, Damir; Kostková, Hana; Petrova, Svetlana; Chytil, Petr; Etrych, Tomáš; Kereïche, Sami; Raška, Ivan; Weitz, David A.; Filippov, Sergey K.

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Klepac, D., Kostková, H., Petrova, S., Chytil, P., Etrych, T., Kereïche, S. ... Filippov, S. K. (2018). Interaction of spin-labeled HPMA-based nanoparticles with human blood plasma proteins – the introduction of protein-corona-free polymer nanomedicine. Nanoscale, 10. (13), 6194-6204. doi: 10.1039/c7nr09355a

Klepac, Damir, et al. "Interaction of spin-labeled HPMA-based nanoparticles with human blood plasma proteins – the introduction of protein-corona-free polymer nanomedicine." Nanoscale, vol. 10, br. 13, 2018, str. 6194-6204. https://doi.org/10.1039/c7nr09355a

Klepac, Damir, Hana Kostková, Svetlana Petrova, Petr Chytil, Tomáš Etrych, Sami Kereïche, Ivan Raška, David A. Weitz i Sergey K. Filippov. "Interaction of spin-labeled HPMA-based nanoparticles with human blood plasma proteins – the introduction of protein-corona-free polymer nanomedicine." Nanoscale 10, br. 13 (2018): 6194-6204. https://doi.org/10.1039/c7nr09355a

Klepac, D., et al. (2018) 'Interaction of spin-labeled HPMA-based nanoparticles with human blood plasma proteins – the introduction of protein-corona-free polymer nanomedicine', Nanoscale, 10(13), str. 6194-6204. doi: 10.1039/c7nr09355a

Klepac D, Kostková H, Petrova S, Chytil P, Etrych T, Kereïche S, i sur.. Interaction of spin-labeled HPMA-based nanoparticles with human blood plasma proteins – the introduction of protein-corona-free polymer nanomedicine. Nanoscale [Internet]. 2018. [pristupljeno 13.11.2024.];10(13):6194-6204. doi: 10.1039/c7nr09355a

D. Klepac, et al., "Interaction of spin-labeled HPMA-based nanoparticles with human blood plasma proteins – the introduction of protein-corona-free polymer nanomedicine", Nanoscale, vol. 10, br. 13, str. 6194-6204, 2018. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:909513. [Citirano: 13.11.2024.]

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Naslov (engleski)Interaction of spin-labeled HPMA-based nanoparticles with human blood plasma proteins – the introduction of protein-corona-free polymer nanomedicine
AutorDamir Klepac
AutorHana Kostková
AutorSvetlana Petrova
AutorPetr Chytil
AutorTomáš Etrych
AutorSami Kereïche
AutorIvan Raška
AutorDavid A. Weitz
AutorSergey K. Filippov
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za medicinsku kemiju, biokemiju i kliničku kemiju)
Znanstveno / umjetničko
područje, polje i grana		PRIRODNE ZNANOSTI
Kemija
Znanstveno / umjetničko
područje, polje i grana		TEHNIČKE ZNANOSTI
Kemijsko inženjerstvo
Sažetak (engleski)
In this paper, we revised the current understanding of the protein corona that is created on the surface of nanoparticles in blood plasma after an intravenous injection. We have focused on nanoparticles that have a proven therapeutic outcome. These nanoparticles are based on two types of biocompatible amphiphilic copolymers based on N-(2- hydroxypropyl)methacrylamide (HPMA): a block copolymer, poly(ε-caprolactone) (PCL)-b- poly(HPMA), and a statistical HPMA copolymer bearing cholesterol moieties, which have been tested both in vitro and in vivo. We studied the interaction of nanoparticles with blood plasma and selected blood plasma proteins by electron paramagnetic resonance (EPR), isothermal titration calorimetry, dynamic light scattering, and cryo-transmission electron microscopy. The copolymers were labeled with TEMPO radicals at the end of hydrophobic PCL or along the hydrophilic HPMA chains to monitor changes in polymer chain dynamics caused by protein adsorption. By EPR and other methods, we were able to probe specific interactions between nanoparticles and blood proteins, specifically low- and high-density lipoproteins, immunoglobulin G, human serum albumin (HSA), and human plasma. It was found that individual proteins and plasma have very low binding affinity to nanoparticles. We observed no hard corona around HPMA-based nanoparticles ; with the exception of HSA the proteins showed no detectable binding to the nanoparticles. Our study confirms that a classical “hard corona–soft corona” paradigm is not valid for all types of nanoparticles and each system has a unique protein corona that is determined by the nature of the NP material
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaNanoscale
Brojčani podacivol. 10, br. 13, str. 6194-6204
p-ISSN2040-3364
DOIhttps://doi.org/10.1039/c7nr09355a
URN:NBNurn:nbn:hr:184:909513
Datum objave publikacije2018
URL dokumentahttp://bib.irb.hr/1049788
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje-Nekomercijalno 3.0 (CC BY-NC 3.0)
Datum i vrijeme pohrane2022-02-23 16:06:06