prikaz prve stranice dokumenta Interaction of spin-labeled HPMA-based nanoparticles with human blood plasma proteins – the introduction of protein-corona-free polymer nanomedicine
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Scientific paper - Original scientific paper
Interaction of spin-labeled HPMA-based nanoparticles with human blood plasma proteins – the introduction of protein-corona-free polymer nanomedicine
Nanoscale, 10 (2018), 13; 6194-6204. https://doi.org/10.1039/c7nr09355a
Klepac, Damir; Kostková, Hana; Petrova, Svetlana; Chytil, Petr; Etrych, Tomáš; Kereïche, Sami; Raška, Ivan; Weitz, David A.; Filippov, Sergey K.

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Klepac, D., Kostková, H., Petrova, S., Chytil, P., Etrych, T., Kereïche, S. ... Filippov, S. K. (2018). Interaction of spin-labeled HPMA-based nanoparticles with human blood plasma proteins – the introduction of protein-corona-free polymer nanomedicine. Nanoscale, 10. (13), 6194-6204. doi: 10.1039/c7nr09355a

Klepac, Damir, et al. "Interaction of spin-labeled HPMA-based nanoparticles with human blood plasma proteins – the introduction of protein-corona-free polymer nanomedicine." Nanoscale, vol. 10, no. 13, 2018, pp. 6194-6204. https://doi.org/10.1039/c7nr09355a

Klepac, Damir, Hana Kostková, Svetlana Petrova, Petr Chytil, Tomáš Etrych, Sami Kereïche, Ivan Raška, David A. Weitz and Sergey K. Filippov. "Interaction of spin-labeled HPMA-based nanoparticles with human blood plasma proteins – the introduction of protein-corona-free polymer nanomedicine." Nanoscale 10, no. 13 (2018): 6194-6204. https://doi.org/10.1039/c7nr09355a

Klepac, D., et al. (2018) 'Interaction of spin-labeled HPMA-based nanoparticles with human blood plasma proteins – the introduction of protein-corona-free polymer nanomedicine', Nanoscale, 10(13), pp. 6194-6204. doi: 10.1039/c7nr09355a

Klepac D, Kostková H, Petrova S, Chytil P, Etrych T, Kereïche S, and sur.. Interaction of spin-labeled HPMA-based nanoparticles with human blood plasma proteins – the introduction of protein-corona-free polymer nanomedicine. Nanoscale [Internet]. 2018 [cited 2024 November 22];10(13):6194-6204. doi: 10.1039/c7nr09355a

D. Klepac, et al., "Interaction of spin-labeled HPMA-based nanoparticles with human blood plasma proteins – the introduction of protein-corona-free polymer nanomedicine", Nanoscale, vol. 10, no. 13, pp. 6194-6204, 2018. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:909513. [Accessed: 22 November 2024]

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Title (english)Interaction of spin-labeled HPMA-based nanoparticles with human blood plasma proteins – the introduction of protein-corona-free polymer nanomedicine
AuthorDamir Klepac
AuthorHana Kostková
AuthorSvetlana Petrova
AuthorPetr Chytil
AuthorTomáš Etrych
AuthorSami Kereïche
AuthorIvan Raška
AuthorDavid A. Weitz
AuthorSergey K. Filippov
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Chemistry and Biochemistry)
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Scientific / art field,
discipline and subdiscipline		TECHNICAL SCIENCES
Chemical Engineering
Abstract (english)
In this paper, we revised the current understanding of the protein corona that is created on the surface of nanoparticles in blood plasma after an intravenous injection. We have focused on nanoparticles that have a proven therapeutic outcome. These nanoparticles are based on two types of biocompatible amphiphilic copolymers based on N-(2- hydroxypropyl)methacrylamide (HPMA): a block copolymer, poly(ε-caprolactone) (PCL)-b- poly(HPMA), and a statistical HPMA copolymer bearing cholesterol moieties, which have been tested both in vitro and in vivo. We studied the interaction of nanoparticles with blood plasma and selected blood plasma proteins by electron paramagnetic resonance (EPR), isothermal titration calorimetry, dynamic light scattering, and cryo-transmission electron microscopy. The copolymers were labeled with TEMPO radicals at the end of hydrophobic PCL or along the hydrophilic HPMA chains to monitor changes in polymer chain dynamics caused by protein adsorption. By EPR and other methods, we were able to probe specific interactions between nanoparticles and blood proteins, specifically low- and high-density lipoproteins, immunoglobulin G, human serum albumin (HSA), and human plasma. It was found that individual proteins and plasma have very low binding affinity to nanoparticles. We observed no hard corona around HPMA-based nanoparticles ; with the exception of HSA the proteins showed no detectable binding to the nanoparticles. Our study confirms that a classical “hard corona–soft corona” paradigm is not valid for all types of nanoparticles and each system has a unique protein corona that is determined by the nature of the NP material
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleNanoscale
Numberingvol. 10, no. 13, pp. 6194-6204
p-ISSN2040-3364
DOIhttps://doi.org/10.1039/c7nr09355a
URN:NBNurn:nbn:hr:184:909513
Publication2018
Document URLhttp://bib.irb.hr/1049788
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution-NonCommercial 3.0 Unported (CC BY-NC 3.0) icon
Created on2022-02-23 16:06:06