Tumours of glial origin are the most common and most important primary tumours of the central nervous system (CNS). According to the latest revision of the CNS tumour classification by the World Health Organization from 2016, glial tumours are divided into: diffuse astrocyte tumours, oligodendroglial tumours, local astrocyte tumours, ependymal tumours, and other gliomas. Nectins are a family of immunoglobulin (Ig) -like, Ca2 + independent, cellular adhesion molecules, which play an important role in a number of cellular functions. The family contains multiple members whose expression differs on normal and tumour cells. Apart from the important role in the proliferation, differentiation, migration and adhesion of the cells, these molecules also play a significant role in the regulation of the immune response. Therefore, the purpose of this study was to better understand and compare the expression of two members of the adhesion molecules, nectin-2 and nectin-4 on glial tumors of different degree of differentiation. The results would contribute to a better knowledge of the distribution of nectin-2 and nectin-4 in glial tumors, glial tumors of different differentiation, the possible importance of these molecules in tumorigenesis and the potential of these molecules in targeted immunotherapy. By analyzing our results, we concluded that both nectin-2 and nectin-4 do express in glial tumours, but this expression is heterogeneous, both between different tumours and within individual tumours, with a stronger expression of nectin-2 compared to nectin-4.