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Degradation of Cellular miR-27 by a Novel, Highly Abundant Viral Transcript Is Important for Efficient Virus Replication In Vivo
PLoS Pathogens, 8 (2012), 2; e1002510. https://doi.org/10.1371/journal.ppat.1002510
Marcinowski, Lisa; Tanguy, Mélanie; Krmpotić, Astrid; Rädle, Bernd; Lisnić, Vanda J.; Tuddenham, Lee; Chane-Woon-Ming, Béatrice; Ruzsics, Zsolt; Erhard, Florian; Benkartek, Corinna; Babić, Marina; Zimmer, Ralf; Trgovcich, Joanne; Koszinowski, Ulrich H.; Jonjić, Stipan; Pfeffer, Sébastien; Dölken, Lars Više autora...

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Marcinowski, L., Tanguy, M., Krmpotić, A., Rädle, B., Lisnić, V. J., Tuddenham, L. ... Dölken, L. (2012). Degradation of Cellular miR-27 by a Novel, Highly Abundant Viral Transcript Is Important for Efficient Virus Replication In Vivo. PLoS Pathogens, 8. (2). doi: 10.1371/journal.ppat.1002510

Marcinowski, Lisa, et al. "Degradation of Cellular miR-27 by a Novel, Highly Abundant Viral Transcript Is Important for Efficient Virus Replication In Vivo." PLoS Pathogens, vol. 8, br. 2, 2012. https://doi.org/10.1371/journal.ppat.1002510

Marcinowski, Lisa, Mélanie Tanguy, Astrid Krmpotić, Bernd Rädle, Vanda J. Lisnić, Lee Tuddenham, Béatrice Chane Woon Ming, et al. "Degradation of Cellular miR-27 by a Novel, Highly Abundant Viral Transcript Is Important for Efficient Virus Replication In Vivo." PLoS Pathogens 8, br. 2 (2012). https://doi.org/10.1371/journal.ppat.1002510

Marcinowski, L., et al. (2012) 'Degradation of Cellular miR-27 by a Novel, Highly Abundant Viral Transcript Is Important for Efficient Virus Replication In Vivo', PLoS Pathogens, 8(2). doi: 10.1371/journal.ppat.1002510

Marcinowski L, Tanguy M, Krmpotić A, Rädle B, Lisnić VJ, Tuddenham L, i sur.. Degradation of Cellular miR-27 by a Novel, Highly Abundant Viral Transcript Is Important for Efficient Virus Replication In Vivo. PLoS Pathogens [Internet]. 09.02.2012. [pristupljeno 24.12.2024.];8(2). doi: 10.1371/journal.ppat.1002510

L. Marcinowski, et al., "Degradation of Cellular miR-27 by a Novel, Highly Abundant Viral Transcript Is Important for Efficient Virus Replication In Vivo", PLoS Pathogens, vol. 8, br. 2, Veljača 2012. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:463773. [Citirano: 24.12.2024.]

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Naslov (engleski)Degradation of Cellular miR-27 by a Novel, Highly Abundant Viral Transcript Is Important for Efficient Virus Replication In Vivo
AutorLisa Marcinowski
AutorMélanie Tanguy
AutorAstrid Krmpotić
AutorBernd Rädle
AutorVanda J. Lisnić
AutorLee Tuddenham
AutorBéatrice Chane-Woon-Ming
AutorZsolt Ruzsics
AutorFlorian Erhard
AutorCorinna Benkartek
AutorMarina Babić
AutorRalf Zimmer
AutorJoanne Trgovcich
AutorUlrich H. Koszinowski
AutorStipan Jonjić
AutorSébastien Pfeffer
AutorLars Dölken
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za histologiju i embriologiju)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Sažetak (engleski)
Cytomegaloviruses express large amounts of viral miRNAs during lytic infection, yet, they only modestly alter the cellular miRNA profile. The most prominent alteration upon lytic murine cytomegalovirus (MCMV) infection is the rapid degradation of the cellular miR-27a and miR-27b. Here, we report that this regulation is mediated by the ∼1.7 kb spliced and highly abundant MCMV m169 transcript. Specificity to miR-27a/b is mediated by a single, apparently optimized, miRNA binding site located in its 3'-UTR. This site is easily and efficiently retargeted to other cellular and viral miRNAs by target site replacement. Expression of the 3'-UTR of m169 by an adenoviral vector was sufficient to mediate its function, indicating that no other viral factors are essential in this process. Degradation of miR-27a/b was found to be accompanied by 3'-tailing and -trimming. Despite its dramatic effect on miRNA stability, we found this interaction to be mutual, indicating potential regulation of m169 by miR-27a/b. Most interestingly, three mutant viruses no longer able to target miR-27a/b, either due to miRNA target site disruption or target site replacement, showed significant attenuation in multiple organs as early as 4 days post infection, indicating that degradation of miR-27a/b is important for efficient MCMV replication in vivo.
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaPLoS Pathogens
Brojčani podacivol. 8, br. 2, e1002510
e-ISSN1553-7374
DOIhttps://doi.org/10.1371/journal.ppat.1002510
URN:NBNurn:nbn:hr:184:463773
Datum objave publikacije2012-02-09
ProjektŠifra: 260767
Naziv (engleski): Roles of non-coding RNAs in viral infections in mammals
Kratica: NCRNAVIR
Pravna nadležnost: eu
Financijer: EC
Linija financiranja: FP7
URL dokumentahttp://dx.plos.org/10.1371/journal.ppat.1002510
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje-Nekomercijalno-Bez prerada 4.0 međunarodna (CC BY-NC-ND 4.0)
Datum i vrijeme pohrane2018-11-08 11:05:43