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Scientific paper - Original scientific paper
Degradation of Cellular miR-27 by a Novel, Highly Abundant Viral Transcript Is Important for Efficient Virus Replication In Vivo
PLoS Pathogens, 8 (2012), 2; e1002510. https://doi.org/10.1371/journal.ppat.1002510
Marcinowski, Lisa; Tanguy, Mélanie; Krmpotić, Astrid; Rädle, Bernd; Lisnić, Vanda J.; Tuddenham, Lee; Chane-Woon-Ming, Béatrice; Ruzsics, Zsolt; Erhard, Florian; Benkartek, Corinna; Babić, Marina; Zimmer, Ralf; Trgovcich, Joanne; Koszinowski, Ulrich H.; Jonjić, Stipan; Pfeffer, Sébastien; Dölken, Lars More authors...

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Marcinowski, L., Tanguy, M., Krmpotić, A., Rädle, B., Lisnić, V. J., Tuddenham, L. ... Dölken, L. (2012). Degradation of Cellular miR-27 by a Novel, Highly Abundant Viral Transcript Is Important for Efficient Virus Replication In Vivo. PLoS Pathogens, 8. (2). doi: 10.1371/journal.ppat.1002510

Marcinowski, Lisa, et al. "Degradation of Cellular miR-27 by a Novel, Highly Abundant Viral Transcript Is Important for Efficient Virus Replication In Vivo." PLoS Pathogens, vol. 8, no. 2, 2012. https://doi.org/10.1371/journal.ppat.1002510

Marcinowski, Lisa, Mélanie Tanguy, Astrid Krmpotić, Bernd Rädle, Vanda J. Lisnić, Lee Tuddenham, Béatrice Chane Woon Ming, et al. "Degradation of Cellular miR-27 by a Novel, Highly Abundant Viral Transcript Is Important for Efficient Virus Replication In Vivo." PLoS Pathogens 8, no. 2 (2012). https://doi.org/10.1371/journal.ppat.1002510

Marcinowski, L., et al. (2012) 'Degradation of Cellular miR-27 by a Novel, Highly Abundant Viral Transcript Is Important for Efficient Virus Replication In Vivo', PLoS Pathogens, 8(2). doi: 10.1371/journal.ppat.1002510

Marcinowski L, Tanguy M, Krmpotić A, Rädle B, Lisnić VJ, Tuddenham L, and sur.. Degradation of Cellular miR-27 by a Novel, Highly Abundant Viral Transcript Is Important for Efficient Virus Replication In Vivo. PLoS Pathogens [Internet]. 2012 February 09 [cited 2024 December 25];8(2). doi: 10.1371/journal.ppat.1002510

L. Marcinowski, et al., "Degradation of Cellular miR-27 by a Novel, Highly Abundant Viral Transcript Is Important for Efficient Virus Replication In Vivo", PLoS Pathogens, vol. 8, no. 2, February 2012. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:463773. [Accessed: 25 December 2024]

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Metadata
Title (english)Degradation of Cellular miR-27 by a Novel, Highly Abundant Viral Transcript Is Important for Efficient Virus Replication In Vivo
AuthorLisa Marcinowski
AuthorMélanie Tanguy
AuthorAstrid Krmpotić
AuthorBernd Rädle
AuthorVanda J. Lisnić
AuthorLee Tuddenham
AuthorBéatrice Chane-Woon-Ming
AuthorZsolt Ruzsics
AuthorFlorian Erhard
AuthorCorinna Benkartek
AuthorMarina Babić
AuthorRalf Zimmer
AuthorJoanne Trgovcich
AuthorUlrich H. Koszinowski
AuthorStipan Jonjić
AuthorSébastien Pfeffer
AuthorLars Dölken
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Histology and Embryology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
Cytomegaloviruses express large amounts of viral miRNAs during lytic infection, yet, they only modestly alter the cellular miRNA profile. The most prominent alteration upon lytic murine cytomegalovirus (MCMV) infection is the rapid degradation of the cellular miR-27a and miR-27b. Here, we report that this regulation is mediated by the ∼1.7 kb spliced and highly abundant MCMV m169 transcript. Specificity to miR-27a/b is mediated by a single, apparently optimized, miRNA binding site located in its 3'-UTR. This site is easily and efficiently retargeted to other cellular and viral miRNAs by target site replacement. Expression of the 3'-UTR of m169 by an adenoviral vector was sufficient to mediate its function, indicating that no other viral factors are essential in this process. Degradation of miR-27a/b was found to be accompanied by 3'-tailing and -trimming. Despite its dramatic effect on miRNA stability, we found this interaction to be mutual, indicating potential regulation of m169 by miR-27a/b. Most interestingly, three mutant viruses no longer able to target miR-27a/b, either due to miRNA target site disruption or target site replacement, showed significant attenuation in multiple organs as early as 4 days post infection, indicating that degradation of miR-27a/b is important for efficient MCMV replication in vivo.
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titlePLoS Pathogens
Numberingvol. 8, no. 2, e1002510
e-ISSN1553-7374
DOIhttps://doi.org/10.1371/journal.ppat.1002510
URN:NBNurn:nbn:hr:184:463773
Publication2012-02-09
ProjectNumber: 260767
Title (english): Roles of non-coding RNAs in viral infections in mammals
Acronym: NCRNAVIR
Jurisdiction: eu
Funding stream: FP7
Document URLhttp://dx.plos.org/10.1371/journal.ppat.1002510
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) icon
Created on2018-11-08 11:05:43