Cirrhosis is irreversible chronic liver disease characterized by damage of liver tissue, and progressive deterioration of liver function. The most common cause of the disease is excessive alcohol consumption. Development and level of alcoholic liver damage depends on the amount of alcohol consumed, the length of consumption, social status, gender, and genetic factors. Recent evidence suggests an association between tumor necrosis factor alpha (TNF-α) with the development of liver cirrhosis. The aim of this study was to investigate the influence of the polymorphism of TNF-α-308 on the occurrence of cirrhosis. The study included 123 patients with cirrhosis and the control group consisted of 246 healthy subjects. Polymorphism of TNF-α-308 G to A was determined by polymerase chain reaction followed by digestion with the restriction enzyme Nco I and checks by agarose gel electrophoresis. The frequency of TNF-α-308 genotypes and alleles did not differ significantly (p>0,05) between patients with alcoholic liver cirrhosis and the control group. The distribution of genotypes between the sexes also were not significantly different (p>0,005). The paper also presents some biochemical parameters (γ–GT, AST, alkaline phosphatase, albumin) that are used in the diagnosis of alcoholic liver disease. There was no significant correlation (p>0,05) between TNF-α-308 genotypes and biochemical parameters. Based on the results, we can conclude that the polymorphism of TNF-308-α gene doesnt have a key role in pathogenesis of alcoholic liver cirrhosis in our patients.