prikaz prve stranice dokumenta Human Cytomegalovirus Nuclear Capsids Associate with the Core Nuclear Egress Complex and the Viral Protein Kinase pUL97
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Human Cytomegalovirus Nuclear Capsids Associate with the Core Nuclear Egress Complex and the Viral Protein Kinase pUL97
Viruses, 10 (2018), 1; 35. https://doi.org/10.3390/v10010035
Milbrandt, Jens; Sonntag, Eric; Wagner, Sabrina; Strojan, Hanife; Wangen, Christina; Lenac Roviš, Tihana; Lisnić, Berislav; Jonjić, Stipan; Sticht, Heinrich; Britt, William J.; Schlotzer-Schrehardt, Ursula; Marschall, Manfred

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Milbrandt, J., Sonntag, E., Wagner, S., Strojan, H., Wangen, C., Lenac Roviš, T. ... Marschall, M. (2018). Human Cytomegalovirus Nuclear Capsids Associate with the Core Nuclear Egress Complex and the Viral Protein Kinase pUL97. Viruses, 10. (1). doi: 10.3390/v10010035

Milbrandt, Jens, et al. "Human Cytomegalovirus Nuclear Capsids Associate with the Core Nuclear Egress Complex and the Viral Protein Kinase pUL97." Viruses, vol. 10, br. 1, 2018. https://doi.org/10.3390/v10010035

Milbrandt, Jens, Eric Sonntag, Sabrina Wagner, Hanife Strojan, Christina Wangen, Tihana Lenac Roviš, Berislav Lisnić, et al. "Human Cytomegalovirus Nuclear Capsids Associate with the Core Nuclear Egress Complex and the Viral Protein Kinase pUL97." Viruses 10, br. 1 (2018). https://doi.org/10.3390/v10010035

Milbrandt, J., et al. (2018) 'Human Cytomegalovirus Nuclear Capsids Associate with the Core Nuclear Egress Complex and the Viral Protein Kinase pUL97', Viruses, 10(1). doi: 10.3390/v10010035

Milbrandt J, Sonntag E, Wagner S, Strojan H, Wangen C, Lenac Roviš T, i sur.. Human Cytomegalovirus Nuclear Capsids Associate with the Core Nuclear Egress Complex and the Viral Protein Kinase pUL97. Viruses [Internet]. 13.01.2018. [pristupljeno 14.11.2024.];10(1). doi: 10.3390/v10010035

J. Milbrandt, et al., "Human Cytomegalovirus Nuclear Capsids Associate with the Core Nuclear Egress Complex and the Viral Protein Kinase pUL97", Viruses, vol. 10, br. 1, Siječanj 2018. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:075229. [Citirano: 14.11.2024.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:184:075229
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Naslov (engleski)Human Cytomegalovirus Nuclear Capsids Associate with the Core Nuclear Egress Complex and the Viral Protein Kinase pUL97
AutorJens Milbrandt
AutorEric Sonntag
AutorSabrina Wagner
AutorHanife Strojan
AutorChristina Wangen
AutorTihana Lenac Roviš
AutorBerislav Lisnić
AutorStipan Jonjić
AutorHeinrich Sticht
AutorWilliam J. Britt
AutorUrsula Schlotzer-Schrehardt
AutorManfred Marschall
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za histologiju i embriologiju)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Sažetak (engleski)
Abstract: The nuclear phase of herpesvirus replication is regulated through the formation of regulatory multi-component protein complexes. Viral genomic replication is followed by nuclear capsid assembly, DNA encapsidation and nuclear egress. The latter has been studied intensely pointing to the formation of a viral core nuclear egress complex (NEC) that recruits a multimeric assembly of viral and cellular factors for the reorganization of the nuclear envelope. To date, the mechanism of the association of human cytomegalovirus (HCMV) capsids with the NEC, which in turn initiates the specific steps of nuclear capsid budding, remains undefined. Here, we provide electron microscopy-based data demonstrating the association of both nuclear capsids and NEC proteins at nuclear lamina budding sites. Specifically, immunogold labelling of the core NEC constituent pUL53 and NEC-associated viral kinase pUL97 suggested an intranuclear NEC-capsid interaction. Staining patterns with phospho-specific lamin A/C antibodies are compatible with earlier postulates of targeted capsid egress at lamina-depleted areas. Important data were provided by co-immunoprecipitation and in vitro kinase analyses using lysates from HCMV-infected cells, nuclear fractions, or infectious virions. Data strongly suggest that nuclear capsids interact with pUL53 and pUL97. Combined, the findings support a refined concept of HCMV nuclear trafficking and NEC-capsid interaction.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaViruses
Brojčani podacivol. 10, br. 1, 35
e-ISSN1999-4915
DOIhttps://doi.org/10.3390/v10010035
URN:NBNurn:nbn:hr:184:075229
Datum objave publikacije2018-01-13
ProjektŠifra: 062-0621261-1263
Naziv (hrvatski): Molekularni mehanizmi citomegalovirusnog izmicanja imunološkom nadzoru
Voditelj: Stipan Jonjić
Pravna nadležnost: Hrvatska
Financijer: MZOS
Linija financiranja: ZP
URL dokumentahttp://www.mdpi.com/1999-4915/10/1/35
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje-Nekomercijalno-Bez prerada 4.0 međunarodna (CC BY-NC-ND 4.0)
Datum i vrijeme pohrane2018-02-11 13:31:33