prikaz prve stranice dokumenta Human Cytomegalovirus Nuclear Capsids Associate with the Core Nuclear Egress Complex and the Viral Protein Kinase pUL97
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Scientific paper - Original scientific paper
Human Cytomegalovirus Nuclear Capsids Associate with the Core Nuclear Egress Complex and the Viral Protein Kinase pUL97
Viruses, 10 (2018), 1; 35. https://doi.org/10.3390/v10010035
Milbrandt, Jens; Sonntag, Eric; Wagner, Sabrina; Strojan, Hanife; Wangen, Christina; Lenac Roviš, Tihana; Lisnić, Berislav; Jonjić, Stipan; Sticht, Heinrich; Britt, William J.; Schlotzer-Schrehardt, Ursula; Marschall, Manfred

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Milbrandt, J., Sonntag, E., Wagner, S., Strojan, H., Wangen, C., Lenac Roviš, T. ... Marschall, M. (2018). Human Cytomegalovirus Nuclear Capsids Associate with the Core Nuclear Egress Complex and the Viral Protein Kinase pUL97. Viruses, 10. (1). doi: 10.3390/v10010035

Milbrandt, Jens, et al. "Human Cytomegalovirus Nuclear Capsids Associate with the Core Nuclear Egress Complex and the Viral Protein Kinase pUL97." Viruses, vol. 10, no. 1, 2018. https://doi.org/10.3390/v10010035

Milbrandt, Jens, Eric Sonntag, Sabrina Wagner, Hanife Strojan, Christina Wangen, Tihana Lenac Roviš, Berislav Lisnić, et al. "Human Cytomegalovirus Nuclear Capsids Associate with the Core Nuclear Egress Complex and the Viral Protein Kinase pUL97." Viruses 10, no. 1 (2018). https://doi.org/10.3390/v10010035

Milbrandt, J., et al. (2018) 'Human Cytomegalovirus Nuclear Capsids Associate with the Core Nuclear Egress Complex and the Viral Protein Kinase pUL97', Viruses, 10(1). doi: 10.3390/v10010035

Milbrandt J, Sonntag E, Wagner S, Strojan H, Wangen C, Lenac Roviš T, and sur.. Human Cytomegalovirus Nuclear Capsids Associate with the Core Nuclear Egress Complex and the Viral Protein Kinase pUL97. Viruses [Internet]. 2018 January 13 [cited 2024 November 21];10(1). doi: 10.3390/v10010035

J. Milbrandt, et al., "Human Cytomegalovirus Nuclear Capsids Associate with the Core Nuclear Egress Complex and the Viral Protein Kinase pUL97", Viruses, vol. 10, no. 1, January 2018. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:075229. [Accessed: 21 November 2024]

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Metadata
Title (english)Human Cytomegalovirus Nuclear Capsids Associate with the Core Nuclear Egress Complex and the Viral Protein Kinase pUL97
AuthorJens Milbrandt
AuthorEric Sonntag
AuthorSabrina Wagner
AuthorHanife Strojan
AuthorChristina Wangen
AuthorTihana Lenac Roviš
AuthorBerislav Lisnić
AuthorStipan Jonjić
AuthorHeinrich Sticht
AuthorWilliam J. Britt
AuthorUrsula Schlotzer-Schrehardt
AuthorManfred Marschall
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Histology and Embryology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
Abstract: The nuclear phase of herpesvirus replication is regulated through the formation of regulatory multi-component protein complexes. Viral genomic replication is followed by nuclear capsid assembly, DNA encapsidation and nuclear egress. The latter has been studied intensely pointing to the formation of a viral core nuclear egress complex (NEC) that recruits a multimeric assembly of viral and cellular factors for the reorganization of the nuclear envelope. To date, the mechanism of the association of human cytomegalovirus (HCMV) capsids with the NEC, which in turn initiates the specific steps of nuclear capsid budding, remains undefined. Here, we provide electron microscopy-based data demonstrating the association of both nuclear capsids and NEC proteins at nuclear lamina budding sites. Specifically, immunogold labelling of the core NEC constituent pUL53 and NEC-associated viral kinase pUL97 suggested an intranuclear NEC-capsid interaction. Staining patterns with phospho-specific lamin A/C antibodies are compatible with earlier postulates of targeted capsid egress at lamina-depleted areas. Important data were provided by co-immunoprecipitation and in vitro kinase analyses using lysates from HCMV-infected cells, nuclear fractions, or infectious virions. Data strongly suggest that nuclear capsids interact with pUL53 and pUL97. Combined, the findings support a refined concept of HCMV nuclear trafficking and NEC-capsid interaction.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleViruses
Numberingvol. 10, no. 1, 35
e-ISSN1999-4915
DOIhttps://doi.org/10.3390/v10010035
URN:NBNurn:nbn:hr:184:075229
Publication2018-01-13
ProjectNumber: 062-0621261-1263
Title (croatian): Molekularni mehanizmi citomegalovirusnog izmicanja imunološkom nadzoru
Leader: Stipan Jonjić
Jurisdiction: Croatia
Funding stream: ZP
Document URLhttp://www.mdpi.com/1999-4915/10/1/35
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) icon
Created on2018-02-11 13:31:33