prikaz prve stranice dokumenta Establishment of liquid biopsy procedure for the analysis of circulating cell free DNA, exosomes, RNA and proteins in colorectal cancer and adenoma patients
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Establishment of liquid biopsy procedure for the analysis of circulating cell free DNA, exosomes, RNA and proteins in colorectal cancer and adenoma patients
Scientific Reports, 14 (2024), 1; 26925. https://doi.org/10.1038/s41598-024-78497-x
Čeri, Andrea; Somborac-Bačura, Anita; Fabijanec, Marija; Hulina-Tomašković, Andrea; Matusina, Marko; Detel, Dijana; Verbanac, Donatella; Barišić, Karmela

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Čeri, A., Somborac Bačura, A., Fabijanec, M., Hulina Tomašković, A., Matusina, M., Detel, D. ... Barišić, K. (2024). Establishment of liquid biopsy procedure for the analysis of circulating cell free DNA, exosomes, RNA and proteins in colorectal cancer and adenoma patients. Scientific Reports, 14. (1). doi: 10.1038/s41598-024-78497-x

Čeri, Andrea, et al. "Establishment of liquid biopsy procedure for the analysis of circulating cell free DNA, exosomes, RNA and proteins in colorectal cancer and adenoma patients." Scientific Reports, vol. 14, br. 1, 2024. https://doi.org/10.1038/s41598-024-78497-x

Čeri, Andrea, Anita Somborac Bačura, Marija Fabijanec, Andrea Hulina Tomašković, Marko Matusina, Dijana Detel, Donatella Verbanac i Karmela Barišić. "Establishment of liquid biopsy procedure for the analysis of circulating cell free DNA, exosomes, RNA and proteins in colorectal cancer and adenoma patients." Scientific Reports 14, br. 1 (2024). https://doi.org/10.1038/s41598-024-78497-x

Čeri, A., et al. (2024) 'Establishment of liquid biopsy procedure for the analysis of circulating cell free DNA, exosomes, RNA and proteins in colorectal cancer and adenoma patients', Scientific Reports, 14(1). doi: 10.1038/s41598-024-78497-x

Čeri A, Somborac Bačura A, Fabijanec M, Hulina Tomašković A, Matusina M, Detel D, i sur.. Establishment of liquid biopsy procedure for the analysis of circulating cell free DNA, exosomes, RNA and proteins in colorectal cancer and adenoma patients. Scientific Reports [Internet]. 06.11.2024. [pristupljeno 27.12.2024.];14(1). doi: 10.1038/s41598-024-78497-x

A. Čeri, et al., "Establishment of liquid biopsy procedure for the analysis of circulating cell free DNA, exosomes, RNA and proteins in colorectal cancer and adenoma patients", Scientific Reports, vol. 14, br. 1, Studeni 2024. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:338289. [Citirano: 27.12.2024.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:184:338289
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Naslov (engleski)Establishment of liquid biopsy procedure for the analysis of circulating cell free DNA, exosomes, RNA and proteins in colorectal cancer and adenoma patients
AutorAndrea Čeri
AutorAnita Somborac-Bačura
AutorMarija Fabijanec
AutorAndrea Hulina-Tomašković
AutorMarko Matusina
AutorDijana Detel
AutorDonatella Verbanac
AutorKarmela Barišić
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za medicinsku kemiju, biokemiju i kliničku kemiju)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Medicinska biokemija
Sažetak (engleski)
Abstract Liquid biopsy has an underexplored diagnostic potential in colorectal cancer (CRC). Sufficient quantity and quality of its elements (circulating cell-free DNA (ccfDNA), exosomes and exosomal RNA) are essential for accurate results. The present study aims to establish the optimal protocol for handling liquid biopsy samples. Samples were obtained by collecting peripheral blood from colorectal adenoma patients in CellSave tubes. Plasma was separated within six hours using differential centrifugation and aliquots stored at – 20/– 80 °C until further processing. Three methods for isolation of ccfDNA, and two combinations of kits for isolation of exosomes and exosomal RNA were tested. The quality and quantity of ccfDNA isolates were evaluated. Exosomes were characterised by determining size, concentration, and total and specific protein content. Expression of chosen microRNAs, miR-19a-3p and miR-92-3p, which have been implicated in CRC progression, were determined. The vacuum-column-based kit showed the highest quantities of isolated ccfDNA (P-value 
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaScientific Reports
Brojčani podacivol. 14, br. 1, 26925
e-ISSN2045-2322
DOIhttps://doi.org/10.1038/s41598-024-78497-x
URN:NBNurn:nbn:hr:184:338289
Datum objave publikacije2024-11-06
ProjektŠifra: KK.01.1.1.02.0021
Naziv (hrvatski): Jačanje znanstveno-istraživačkih i inovacijskih kapaciteta Farmaceutsko-biokemijskog fakulteta Sveučilišta u Zagrebu
Naziv (engleski): -
Kratica: FarmInova
Voditelj: Jasmina Lovrić
Pravna nadležnost: eu
Financijer: Europska unija
Linija financiranja: European Regional Development Fund
URL dokumentahttps://www.nature.com/articles/s41598-024-78497-x
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje-Bez prerada 4.0 međunarodna (CC BY-ND 4.0)
Datum i vrijeme pohrane2024-12-09 10:44:38