prikaz prve stranice dokumenta Establishment of liquid biopsy procedure for the analysis of circulating cell free DNA, exosomes, RNA and proteins in colorectal cancer and adenoma patients
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Scientific paper - Original scientific paper
Establishment of liquid biopsy procedure for the analysis of circulating cell free DNA, exosomes, RNA and proteins in colorectal cancer and adenoma patients
Scientific Reports, 14 (2024), 1; 26925. https://doi.org/10.1038/s41598-024-78497-x
Čeri, Andrea; Somborac-Bačura, Anita; Fabijanec, Marija; Hulina-Tomašković, Andrea; Matusina, Marko; Detel, Dijana; Verbanac, Donatella; Barišić, Karmela

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Čeri, A., Somborac Bačura, A., Fabijanec, M., Hulina Tomašković, A., Matusina, M., Detel, D. ... Barišić, K. (2024). Establishment of liquid biopsy procedure for the analysis of circulating cell free DNA, exosomes, RNA and proteins in colorectal cancer and adenoma patients. Scientific Reports, 14. (1). doi: 10.1038/s41598-024-78497-x

Čeri, Andrea, et al. "Establishment of liquid biopsy procedure for the analysis of circulating cell free DNA, exosomes, RNA and proteins in colorectal cancer and adenoma patients." Scientific Reports, vol. 14, no. 1, 2024. https://doi.org/10.1038/s41598-024-78497-x

Čeri, Andrea, Anita Somborac Bačura, Marija Fabijanec, Andrea Hulina Tomašković, Marko Matusina, Dijana Detel, Donatella Verbanac and Karmela Barišić. "Establishment of liquid biopsy procedure for the analysis of circulating cell free DNA, exosomes, RNA and proteins in colorectal cancer and adenoma patients." Scientific Reports 14, no. 1 (2024). https://doi.org/10.1038/s41598-024-78497-x

Čeri, A., et al. (2024) 'Establishment of liquid biopsy procedure for the analysis of circulating cell free DNA, exosomes, RNA and proteins in colorectal cancer and adenoma patients', Scientific Reports, 14(1). doi: 10.1038/s41598-024-78497-x

Čeri A, Somborac Bačura A, Fabijanec M, Hulina Tomašković A, Matusina M, Detel D, and sur.. Establishment of liquid biopsy procedure for the analysis of circulating cell free DNA, exosomes, RNA and proteins in colorectal cancer and adenoma patients. Scientific Reports [Internet]. 2024 November 06 [cited 2025 January 03];14(1). doi: 10.1038/s41598-024-78497-x

A. Čeri, et al., "Establishment of liquid biopsy procedure for the analysis of circulating cell free DNA, exosomes, RNA and proteins in colorectal cancer and adenoma patients", Scientific Reports, vol. 14, no. 1, November 2024. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:338289. [Accessed: 03 January 2025]

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Metadata
Title (english)Establishment of liquid biopsy procedure for the analysis of circulating cell free DNA, exosomes, RNA and proteins in colorectal cancer and adenoma patients
AuthorAndrea Čeri
AuthorAnita Somborac-Bačura
AuthorMarija Fabijanec
AuthorAndrea Hulina-Tomašković
AuthorMarko Matusina
AuthorDijana Detel
AuthorDonatella Verbanac
AuthorKarmela Barišić
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Chemistry and Biochemistry)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Medical Biochemistry
Abstract (english)
Abstract Liquid biopsy has an underexplored diagnostic potential in colorectal cancer (CRC). Sufficient quantity and quality of its elements (circulating cell-free DNA (ccfDNA), exosomes and exosomal RNA) are essential for accurate results. The present study aims to establish the optimal protocol for handling liquid biopsy samples. Samples were obtained by collecting peripheral blood from colorectal adenoma patients in CellSave tubes. Plasma was separated within six hours using differential centrifugation and aliquots stored at – 20/– 80 °C until further processing. Three methods for isolation of ccfDNA, and two combinations of kits for isolation of exosomes and exosomal RNA were tested. The quality and quantity of ccfDNA isolates were evaluated. Exosomes were characterised by determining size, concentration, and total and specific protein content. Expression of chosen microRNAs, miR-19a-3p and miR-92-3p, which have been implicated in CRC progression, were determined. The vacuum-column-based kit showed the highest quantities of isolated ccfDNA (P-value 
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleScientific Reports
Numberingvol. 14, no. 1, 26925
e-ISSN2045-2322
DOIhttps://doi.org/10.1038/s41598-024-78497-x
URN:NBNurn:nbn:hr:184:338289
Publication2024-11-06
ProjectNumber: KK.01.1.1.02.0021
Title (croatian): Jačanje znanstveno-istraživačkih i inovacijskih kapaciteta Farmaceutsko-biokemijskog fakulteta Sveučilišta u Zagrebu
Title (english): -
Acronym: FarmInova
Leader: Jasmina Lovrić
Jurisdiction: eu
Funding stream: European Regional Development Fund
Document URLhttps://www.nature.com/articles/s41598-024-78497-x
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2024-12-09 10:44:38