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Real-Time PCR Method as Diagnostic Tool for Detection of Periodontal Pathogens in Patients with Periodontitis
International Journal of Molecular Sciences, 25 (2024), 10; 5097. https://doi.org/10.3390/ijms25105097
Kuret, Sendi; Kalajzić, Nina; Ruzdjak, Matija; Grahovac, Blaženka; Jezina Buselić, Marina Adriana; Sardelić, Sanda; Delic, Anja; Susak, Lana; Sutlović, Davorka

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Kuret, S., Kalajzić, N., Ruzdjak, M., Grahovac, B., Jezina Buselić, M. A., Sardelić, S. ... Sutlović, D. (2024). Real-Time PCR Method as Diagnostic Tool for Detection of Periodontal Pathogens in Patients with Periodontitis. International Journal of Molecular Sciences, 25. (10). doi: 10.3390/ijms25105097

Kuret, Sendi, et al. "Real-Time PCR Method as Diagnostic Tool for Detection of Periodontal Pathogens in Patients with Periodontitis." International Journal of Molecular Sciences, vol. 25, br. 10, 2024. https://doi.org/10.3390/ijms25105097

Kuret, Sendi, Nina Kalajzić, Matija Ruzdjak, Blaženka Grahovac, Marina Adriana Jezina Buselić, Sanda Sardelić, Anja Delic, Lana Susak i Davorka Sutlović. "Real-Time PCR Method as Diagnostic Tool for Detection of Periodontal Pathogens in Patients with Periodontitis." International Journal of Molecular Sciences 25, br. 10 (2024). https://doi.org/10.3390/ijms25105097

Kuret, S., et al. (2024) 'Real-Time PCR Method as Diagnostic Tool for Detection of Periodontal Pathogens in Patients with Periodontitis', International Journal of Molecular Sciences, 25(10). doi: 10.3390/ijms25105097

Kuret S, Kalajzić N, Ruzdjak M, Grahovac B, Jezina Buselić MA, Sardelić S, i sur.. Real-Time PCR Method as Diagnostic Tool for Detection of Periodontal Pathogens in Patients with Periodontitis. International Journal of Molecular Sciences [Internet]. 07.05.2024. [pristupljeno 02.12.2024.];25(10). doi: 10.3390/ijms25105097

S. Kuret, et al., "Real-Time PCR Method as Diagnostic Tool for Detection of Periodontal Pathogens in Patients with Periodontitis", International Journal of Molecular Sciences, vol. 25, br. 10, Svibanj 2024. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:034561. [Citirano: 02.12.2024.]

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Podaci o radu
Naslov (engleski)Real-Time PCR Method as Diagnostic Tool for Detection of Periodontal Pathogens in Patients with Periodontitis
AutorSendi Kuret
AutorNina Kalajzić
AutorMatija Ruzdjak
AutorBlaženka Grahovac
AutorMarina Adriana Jezina Buselić
AutorSanda Sardelić
AutorAnja Delic
AutorLana Susak
AutorDavorka Sutlović
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za opću patologiju i patološku anatomiju)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Dentalna medicina
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Kliničke medicinske znanosti
Patologija
Sažetak (engleski)
The most common type of periodontal disease is chronic periodontitis, an inflammatory condition caused by pathogenic bacteria in subgingival plaque. The aim of our study was the development of a real-time PCR test as a diagnostic tool for the detection and differentiation of five periodontopathogenic bacteria, Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Prevotella intermedia, and Treponema denticola, in patients with periodontitis. We compared the results of our in-house method with the micro-IDent® semiquantitative commercially available test based on the PCR hybridization method. DNA was isolated from subgingival plaque samples taken from 50 patients and then analyzed by both methods. Comparing the results of the two methods, they show a specificity of 100% for all bacteria. The sensitivity for A. actinomycetemcomitans was 97.5%, for P. gingivalis 96.88%, and for P. intermedia 95.24%. The sensitivity for Tannerella forsythia and T. denticola was 100%. The Spearman correlation factor of two different measurements was 0.976 for A. actinomycetemcomitans, 0.967 for P. gingivalis, 0.949 for P. intermedia, 0.966 for Tannerella forsythia, and 0.917 for T. denticola. In conclusion, the in-house real-time PCR method developed in our laboratory can provide information about relative amount of five bacterial species present in subgingival plaque in patients with periodontitis. It is likely that such a test could be used in dental diagnostics in assessing the efficacy of any treatment to reduce the bacterial burden.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaInternational Journal of Molecular Sciences
Brojčani podacivol. 25, br. 10, 5097
e-ISSN1422-0067
DOIhttps://doi.org/10.3390/ijms25105097
URN:NBNurn:nbn:hr:184:034561
Datum objave publikacije2024-05-07
URL dokumentahttps://www.mdpi.com/1422-0067/25/10/5097
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 4.0 međunarodna (CC BY 4.0)
Datum i vrijeme pohrane2024-05-08 06:26:56