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Scientific paper - Original scientific paper
Real-Time PCR Method as Diagnostic Tool for Detection of Periodontal Pathogens in Patients with Periodontitis
International Journal of Molecular Sciences, 25 (2024), 10; 5097. https://doi.org/10.3390/ijms25105097
Kuret, Sendi; Kalajzić, Nina; Ruzdjak, Matija; Grahovac, Blaženka; Jezina Buselić, Marina Adriana; Sardelić, Sanda; Delic, Anja; Susak, Lana; Sutlović, Davorka

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Kuret, S., Kalajzić, N., Ruzdjak, M., Grahovac, B., Jezina Buselić, M. A., Sardelić, S. ... Sutlović, D. (2024). Real-Time PCR Method as Diagnostic Tool for Detection of Periodontal Pathogens in Patients with Periodontitis. International Journal of Molecular Sciences, 25. (10). doi: 10.3390/ijms25105097

Kuret, Sendi, et al. "Real-Time PCR Method as Diagnostic Tool for Detection of Periodontal Pathogens in Patients with Periodontitis." International Journal of Molecular Sciences, vol. 25, no. 10, 2024. https://doi.org/10.3390/ijms25105097

Kuret, Sendi, Nina Kalajzić, Matija Ruzdjak, Blaženka Grahovac, Marina Adriana Jezina Buselić, Sanda Sardelić, Anja Delic, Lana Susak and Davorka Sutlović. "Real-Time PCR Method as Diagnostic Tool for Detection of Periodontal Pathogens in Patients with Periodontitis." International Journal of Molecular Sciences 25, no. 10 (2024). https://doi.org/10.3390/ijms25105097

Kuret, S., et al. (2024) 'Real-Time PCR Method as Diagnostic Tool for Detection of Periodontal Pathogens in Patients with Periodontitis', International Journal of Molecular Sciences, 25(10). doi: 10.3390/ijms25105097

Kuret S, Kalajzić N, Ruzdjak M, Grahovac B, Jezina Buselić MA, Sardelić S, and sur.. Real-Time PCR Method as Diagnostic Tool for Detection of Periodontal Pathogens in Patients with Periodontitis. International Journal of Molecular Sciences [Internet]. 2024 May 07 [cited 2024 June 20];25(10). doi: 10.3390/ijms25105097

S. Kuret, et al., "Real-Time PCR Method as Diagnostic Tool for Detection of Periodontal Pathogens in Patients with Periodontitis", International Journal of Molecular Sciences, vol. 25, no. 10, May 2024. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:034561. [Accessed: 20 June 2024]

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Metadata
Title (english)Real-Time PCR Method as Diagnostic Tool for Detection of Periodontal Pathogens in Patients with Periodontitis
AuthorSendi Kuret
AuthorNina Kalajzić
AuthorMatija Ruzdjak
AuthorBlaženka Grahovac
AuthorMarina Adriana Jezina Buselić
AuthorSanda Sardelić
AuthorAnja Delic
AuthorLana Susak
AuthorDavorka Sutlović
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of General Pathology and Pathological Anatomy)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Dental Medicine
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Pathology
Abstract (english)
The most common type of periodontal disease is chronic periodontitis, an inflammatory condition caused by pathogenic bacteria in subgingival plaque. The aim of our study was the development of a real-time PCR test as a diagnostic tool for the detection and differentiation of five periodontopathogenic bacteria, Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Prevotella intermedia, and Treponema denticola, in patients with periodontitis. We compared the results of our in-house method with the micro-IDent® semiquantitative commercially available test based on the PCR hybridization method. DNA was isolated from subgingival plaque samples taken from 50 patients and then analyzed by both methods. Comparing the results of the two methods, they show a specificity of 100% for all bacteria. The sensitivity for A. actinomycetemcomitans was 97.5%, for P. gingivalis 96.88%, and for P. intermedia 95.24%. The sensitivity for Tannerella forsythia and T. denticola was 100%. The Spearman correlation factor of two different measurements was 0.976 for A. actinomycetemcomitans, 0.967 for P. gingivalis, 0.949 for P. intermedia, 0.966 for Tannerella forsythia, and 0.917 for T. denticola. In conclusion, the in-house real-time PCR method developed in our laboratory can provide information about relative amount of five bacterial species present in subgingival plaque in patients with periodontitis. It is likely that such a test could be used in dental diagnostics in assessing the efficacy of any treatment to reduce the bacterial burden.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleInternational Journal of Molecular Sciences
Numberingvol. 25, no. 10, 5097
e-ISSN1422-0067
DOIhttps://doi.org/10.3390/ijms25105097
URN:NBNurn:nbn:hr:184:034561
Publication2024-05-07
Document URLhttps://www.mdpi.com/1422-0067/25/10/5097
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2024-05-08 06:26:56