prikaz prve stranice dokumenta Functional regions of the human cytomegalovirus protein pUL97 involved in nuclear localization and phosphorylation of ganciclovir and pUL97 itself.
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Functional regions of the human cytomegalovirus protein pUL97 involved in nuclear localization and phosphorylation of ganciclovir and pUL97 itself.
Journal of general virology, 79 (1998); 2105-2112. urn:nbn:hr:184:067511
Michel, Detlef; Schaarschmidt, Peter; Wunderlich, Kirsten; Heuschmid, Maria; Simoncini, Lavinia; Mühlberger, Dita; Zimmermann, Albert; Pavić, Ivica; and Mertens, Thomas.

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Michel, D., Schaarschmidt, P., Wunderlich, K., Heuschmid, M., Simoncini, L., Mühlberger, D. ... and Mertens, T. (1998). Functional regions of the human cytomegalovirus protein pUL97 involved in nuclear localization and phosphorylation of ganciclovir and pUL97 itself.. Journal of general virology, 79., 2105-2112. Preuzeto s https://urn.nsk.hr/urn:nbn:hr:184:067511

Michel, Detlef, et al. "Functional regions of the human cytomegalovirus protein pUL97 involved in nuclear localization and phosphorylation of ganciclovir and pUL97 itself.." Journal of general virology, vol. 79, 1998, str. 2105-2112. https://urn.nsk.hr/urn:nbn:hr:184:067511

Michel, Detlef, Peter Schaarschmidt, Kirsten Wunderlich, Maria Heuschmid, Lavinia Simoncini, Dita Mühlberger, Albert Zimmermann, Ivica Pavić i Thomas. and Mertens. "Functional regions of the human cytomegalovirus protein pUL97 involved in nuclear localization and phosphorylation of ganciclovir and pUL97 itself.." Journal of general virology 79 (1998): 2105-2112. https://urn.nsk.hr/urn:nbn:hr:184:067511

Michel, D., et al. (1998) 'Functional regions of the human cytomegalovirus protein pUL97 involved in nuclear localization and phosphorylation of ganciclovir and pUL97 itself.', Journal of general virology, 79, str. 2105-2112. Preuzeto s: https://urn.nsk.hr/urn:nbn:hr:184:067511 (Datum pristupa: 04.12.2024.)

Michel D, Schaarschmidt P, Wunderlich K, Heuschmid M, Simoncini L, Mühlberger D, i sur.. Functional regions of the human cytomegalovirus protein pUL97 involved in nuclear localization and phosphorylation of ganciclovir and pUL97 itself.. Journal of general virology [Internet]. 1998. [pristupljeno 04.12.2024.];79:2105-2112. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:067511

D. Michel, et al., "Functional regions of the human cytomegalovirus protein pUL97 involved in nuclear localization and phosphorylation of ganciclovir and pUL97 itself.", Journal of general virology, vol. 79, str. 2105-2112, 1998. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:067511. [Citirano: 04.12.2024.]

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Naslov (engleski)Functional regions of the human cytomegalovirus protein pUL97 involved in nuclear localization and phosphorylation of ganciclovir and pUL97 itself.
AutorDetlef Michel
AutorPeter Schaarschmidt
AutorKirsten Wunderlich
AutorMaria Heuschmid
AutorLavinia Simoncini
AutorDita Mühlberger
AutorAlbert Zimmermann
AutorIvica Pavić
AutorThomas. and Mertens
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za zarazne bolesti)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Sažetak (engleski)
In order to identify functional regions of the human cytomegalovirus protein pUL97 (i) different 5' fragments of the UL97 open reading frame (ORF) were fused to the coding region of the green fluorescent protein and (ii) recombinant vaccinia viruses (rVV) were generated carrying two full-length and 11 mutated UL97 ORFs. The results indicated the presence of an N-terminal region within pUL97 which changed the intracellular distribution of the fusion proteins. pUL97 was localized in the nucleus, but not in the nucleoli, and was detected in the nuclear matrix fraction. Expression of all pUL97 mutants could be confirmed by Western blot analysis. pUL97- associated ganciclovir (GCV) phosphorylation in rVV-infected cells, determined quantitatively by HPLC analysis, was abolished completely using individual UL97 deletion mutants. Phosphorylation of full-length and some of the mutated pUL97 was detected in cells infected with the rVVs. The UL97 constructs carrying point mutations from GCV-resistant HCMV isolates at positions 460M, 520H, 594V, and the 4 aa deletion 590AACR593, also resulted in decreased but not abolished phosphorylation of GCV in the rVV system, whereas the phosphorylation of pUL97 itself was not influenced. The rVV system is a suitable method for quantitatively testing the functional relevance of pUL97 mutations.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaJournal of general virology
Brojčani podacivol. 79, str. 2105-2112
p-ISSN0022-1317
URN:NBNurn:nbn:hr:184:067511
Datum objave publikacije1998
URL dokumentahttp://bib.irb.hr/811843
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Zaštićeno autorskim pravom.
Datum i vrijeme pohrane2022-04-20 13:55:19