prikaz prve stranice dokumenta Functional regions of the human cytomegalovirus protein pUL97 involved in nuclear localization and phosphorylation of ganciclovir and pUL97 itself.
  Download
PDF 366.03 KB
Scientific paper - Original scientific paper
Functional regions of the human cytomegalovirus protein pUL97 involved in nuclear localization and phosphorylation of ganciclovir and pUL97 itself.
Journal of general virology, 79 (1998); 2105-2112. urn:nbn:hr:184:067511
Michel, Detlef; Schaarschmidt, Peter; Wunderlich, Kirsten; Heuschmid, Maria; Simoncini, Lavinia; Mühlberger, Dita; Zimmermann, Albert; Pavić, Ivica; and Mertens, Thomas.

Cite this document

Michel, D., Schaarschmidt, P., Wunderlich, K., Heuschmid, M., Simoncini, L., Mühlberger, D. ... and Mertens, T. (1998). Functional regions of the human cytomegalovirus protein pUL97 involved in nuclear localization and phosphorylation of ganciclovir and pUL97 itself.. Journal of general virology, 79., 2105-2112. Retrieved from https://urn.nsk.hr/urn:nbn:hr:184:067511

Michel, Detlef, et al. "Functional regions of the human cytomegalovirus protein pUL97 involved in nuclear localization and phosphorylation of ganciclovir and pUL97 itself.." Journal of general virology, vol. 79, 1998, pp. 2105-2112. https://urn.nsk.hr/urn:nbn:hr:184:067511

Michel, Detlef, Peter Schaarschmidt, Kirsten Wunderlich, Maria Heuschmid, Lavinia Simoncini, Dita Mühlberger, Albert Zimmermann, Ivica Pavić and Thomas. and Mertens. "Functional regions of the human cytomegalovirus protein pUL97 involved in nuclear localization and phosphorylation of ganciclovir and pUL97 itself.." Journal of general virology 79 (1998): 2105-2112. https://urn.nsk.hr/urn:nbn:hr:184:067511

Michel, D., et al. (1998) 'Functional regions of the human cytomegalovirus protein pUL97 involved in nuclear localization and phosphorylation of ganciclovir and pUL97 itself.', Journal of general virology, 79, pp. 2105-2112. Available at: https://urn.nsk.hr/urn:nbn:hr:184:067511 (Accessed 31 December 2024)

Michel D, Schaarschmidt P, Wunderlich K, Heuschmid M, Simoncini L, Mühlberger D, and sur.. Functional regions of the human cytomegalovirus protein pUL97 involved in nuclear localization and phosphorylation of ganciclovir and pUL97 itself.. Journal of general virology [Internet]. 1998 [cited 2024 December 31];79:2105-2112. Available at: https://urn.nsk.hr/urn:nbn:hr:184:067511

D. Michel, et al., "Functional regions of the human cytomegalovirus protein pUL97 involved in nuclear localization and phosphorylation of ganciclovir and pUL97 itself.", Journal of general virology, vol. 79, pp. 2105-2112, 1998. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:067511. [Accessed: 31 December 2024]

Please login to the repository to save this object to your list.
Metadata
Title (english)Functional regions of the human cytomegalovirus protein pUL97 involved in nuclear localization and phosphorylation of ganciclovir and pUL97 itself.
AuthorDetlef Michel
AuthorPeter Schaarschmidt
AuthorKirsten Wunderlich
AuthorMaria Heuschmid
AuthorLavinia Simoncini
AuthorDita Mühlberger
AuthorAlbert Zimmermann
AuthorIvica Pavić
AuthorThomas. and Mertens
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Infectious Diseases)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
In order to identify functional regions of the human cytomegalovirus protein pUL97 (i) different 5' fragments of the UL97 open reading frame (ORF) were fused to the coding region of the green fluorescent protein and (ii) recombinant vaccinia viruses (rVV) were generated carrying two full-length and 11 mutated UL97 ORFs. The results indicated the presence of an N-terminal region within pUL97 which changed the intracellular distribution of the fusion proteins. pUL97 was localized in the nucleus, but not in the nucleoli, and was detected in the nuclear matrix fraction. Expression of all pUL97 mutants could be confirmed by Western blot analysis. pUL97- associated ganciclovir (GCV) phosphorylation in rVV-infected cells, determined quantitatively by HPLC analysis, was abolished completely using individual UL97 deletion mutants. Phosphorylation of full-length and some of the mutated pUL97 was detected in cells infected with the rVVs. The UL97 constructs carrying point mutations from GCV-resistant HCMV isolates at positions 460M, 520H, 594V, and the 4 aa deletion 590AACR593, also resulted in decreased but not abolished phosphorylation of GCV in the rVV system, whereas the phosphorylation of pUL97 itself was not influenced. The rVV system is a suitable method for quantitatively testing the functional relevance of pUL97 mutations.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleJournal of general virology
Numberingvol. 79, pp. 2105-2112
p-ISSN0022-1317
URN:NBNurn:nbn:hr:184:067511
Publication1998
Document URLhttp://bib.irb.hr/811843
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2022-04-20 13:55:19