prikaz prve stranice dokumenta Immunophenotyping of a Stromal Vascular Fraction from Microfragmented Lipoaspirate Used in Osteoarthritis Cartilage Treatment and Its Lipoaspirate Counterpart
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Immunophenotyping of a Stromal Vascular Fraction from Microfragmented Lipoaspirate Used in Osteoarthritis Cartilage Treatment and Its Lipoaspirate Counterpart
Genes, 10 (2019), 6; 474. https://doi.org/10.3390/genes10060474
Polančec, Denis; Zenić, Lucija; Hudetz, Damir; Borić, Igor; Jelec, Željko; Rod, Eduard; Vrdoljak, Trpimir; Skelin, Andrea; Plečko, Mihovil; Turkalj, Mirjana; Nogalo, Boro; Primorac, Dragan

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Polančec, D., Zenić, L., Hudetz, D., Borić, I., Jelec, Ž., Rod, E. ... Primorac, D. (2019). Immunophenotyping of a Stromal Vascular Fraction from Microfragmented Lipoaspirate Used in Osteoarthritis Cartilage Treatment and Its Lipoaspirate Counterpart. Genes, 10. (6). doi: 10.3390/genes10060474

Polančec, Denis, et al. "Immunophenotyping of a Stromal Vascular Fraction from Microfragmented Lipoaspirate Used in Osteoarthritis Cartilage Treatment and Its Lipoaspirate Counterpart." Genes, vol. 10, br. 6, 2019. https://doi.org/10.3390/genes10060474

Polančec, Denis, Lucija Zenić, Damir Hudetz, Igor Borić, Željko Jelec, Eduard Rod, Trpimir Vrdoljak, et al. "Immunophenotyping of a Stromal Vascular Fraction from Microfragmented Lipoaspirate Used in Osteoarthritis Cartilage Treatment and Its Lipoaspirate Counterpart." Genes 10, br. 6 (2019). https://doi.org/10.3390/genes10060474

Polančec, D., et al. (2019) 'Immunophenotyping of a Stromal Vascular Fraction from Microfragmented Lipoaspirate Used in Osteoarthritis Cartilage Treatment and Its Lipoaspirate Counterpart', Genes, 10(6). doi: 10.3390/genes10060474

Polančec D, Zenić L, Hudetz D, Borić I, Jelec Ž, Rod E, i sur.. Immunophenotyping of a Stromal Vascular Fraction from Microfragmented Lipoaspirate Used in Osteoarthritis Cartilage Treatment and Its Lipoaspirate Counterpart. Genes [Internet]. 21.06.2019. [pristupljeno 19.11.2024.];10(6). doi: 10.3390/genes10060474

D. Polančec, et al., "Immunophenotyping of a Stromal Vascular Fraction from Microfragmented Lipoaspirate Used in Osteoarthritis Cartilage Treatment and Its Lipoaspirate Counterpart", Genes, vol. 10, br. 6, Lipanj 2019. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:376119. [Citirano: 19.11.2024.]

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Podaci o radu
Naslov (engleski)Immunophenotyping of a Stromal Vascular Fraction from Microfragmented Lipoaspirate Used in Osteoarthritis Cartilage Treatment and Its Lipoaspirate Counterpart
AutorDenis Polančec
AutorLucija Zenić
AutorDamir Hudetz
AutorIgor Borić
AutorŽeljko Jelec
AutorEduard Rod
AutorTrpimir Vrdoljak
AutorAndrea Skelin
AutorMihovil Plečko
AutorMirjana Turkalj
AutorBoro Nogalo
AutorDragan Primorac
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za radiologiju)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Genetika, genomika i proteomika čovjeka
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Kliničke medicinske znanosti
Ortopedija
Sažetak (engleski)
Osteoarthritis (OA) is a degenerative joint disease accompanied by pain and loss of function. Adipose tissue harbors mesenchymal stem/stromal cells (MSC), or medicinal signaling cells as suggested by Caplan (Caplan, 2017), used in autologous transplantation in many clinical settings. The aim of the study was to characterize a stromal vascular fraction from microfragmented lipoaspirate (SVF-MLA) applied for cartilage treatment in OA and compare it to that of autologous lipoaspirate (SVF-LA). Samples were first stained using a DuraClone SC prototype tube for the surface detection of CD31, CD34, CD45, CD73, CD90, CD105, CD146 and LIVE/DEAD Yellow Fixable Stain for dead cell detection, followed by DRAQ7 cell nuclear dye staining, and analyzed by flow cytometry. In SVF-LA and SVF-MLA samples, the following population phenotypes were identified within the CD45- fraction: CD31+CD34+CD73±CD90±CD105±CD146± endothelial progenitors (EP), CD31+CD34-CD73±CD90±CD105-CD146± mature endothelial cells, CD31-CD34-CD73±CD90+CD105-CD146+ pericytes, CD31-CD34+CD73±CD90+CD105-CD146+ transitional pericytes, and CD31-CD34+CD73highCD90+CD105-CD146- supra-adventitial-adipose stromal cells (SA-ASC). The immunophenotyping profile of SVF-MLA was dominated by a reduction of leukocytes and SA-ASC, and an increase in EP, evidencing a marked enrichment of this cell population in the course of adipose tissue microfragmentation. The role of EP in pericyte-primed MSC-mediated tissue healing, as well as the observed hormonal implication, is yet to be investigated.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Prethodno/kratko priopćenje
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaGenes
Brojčani podacivol. 10, br. 6, 474
e-ISSN2073-4425
DOIhttps://doi.org/10.3390/genes10060474
URN:NBNurn:nbn:hr:184:376119
Datum objave publikacije2019-06-21
URL dokumentahttps://www.mdpi.com/2073-4425/10/6/474
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 4.0 međunarodna (CC BY 4.0)
Datum i vrijeme pohrane2019-07-08 07:21:24