prikaz prve stranice dokumenta Immunophenotyping of a Stromal Vascular Fraction from Microfragmented Lipoaspirate Used in Osteoarthritis Cartilage Treatment and Its Lipoaspirate Counterpart
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Scientific paper - Preliminary note / Short communication
Immunophenotyping of a Stromal Vascular Fraction from Microfragmented Lipoaspirate Used in Osteoarthritis Cartilage Treatment and Its Lipoaspirate Counterpart
Genes, 10 (2019), 6; 474. https://doi.org/10.3390/genes10060474
Polančec, Denis; Zenić, Lucija; Hudetz, Damir; Borić, Igor; Jelec, Željko; Rod, Eduard; Vrdoljak, Trpimir; Skelin, Andrea; Plečko, Mihovil; Turkalj, Mirjana; Nogalo, Boro; Primorac, Dragan

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Polančec, D., Zenić, L., Hudetz, D., Borić, I., Jelec, Ž., Rod, E. ... Primorac, D. (2019). Immunophenotyping of a Stromal Vascular Fraction from Microfragmented Lipoaspirate Used in Osteoarthritis Cartilage Treatment and Its Lipoaspirate Counterpart. Genes, 10. (6). doi: 10.3390/genes10060474

Polančec, Denis, et al. "Immunophenotyping of a Stromal Vascular Fraction from Microfragmented Lipoaspirate Used in Osteoarthritis Cartilage Treatment and Its Lipoaspirate Counterpart." Genes, vol. 10, no. 6, 2019. https://doi.org/10.3390/genes10060474

Polančec, Denis, Lucija Zenić, Damir Hudetz, Igor Borić, Željko Jelec, Eduard Rod, Trpimir Vrdoljak, et al. "Immunophenotyping of a Stromal Vascular Fraction from Microfragmented Lipoaspirate Used in Osteoarthritis Cartilage Treatment and Its Lipoaspirate Counterpart." Genes 10, no. 6 (2019). https://doi.org/10.3390/genes10060474

Polančec, D., et al. (2019) 'Immunophenotyping of a Stromal Vascular Fraction from Microfragmented Lipoaspirate Used in Osteoarthritis Cartilage Treatment and Its Lipoaspirate Counterpart', Genes, 10(6). doi: 10.3390/genes10060474

Polančec D, Zenić L, Hudetz D, Borić I, Jelec Ž, Rod E, and sur.. Immunophenotyping of a Stromal Vascular Fraction from Microfragmented Lipoaspirate Used in Osteoarthritis Cartilage Treatment and Its Lipoaspirate Counterpart. Genes [Internet]. 2019 June 21 [cited 2024 December 02];10(6). doi: 10.3390/genes10060474

D. Polančec, et al., "Immunophenotyping of a Stromal Vascular Fraction from Microfragmented Lipoaspirate Used in Osteoarthritis Cartilage Treatment and Its Lipoaspirate Counterpart", Genes, vol. 10, no. 6, June 2019. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:376119. [Accessed: 02 December 2024]

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Metadata
Title (english)Immunophenotyping of a Stromal Vascular Fraction from Microfragmented Lipoaspirate Used in Osteoarthritis Cartilage Treatment and Its Lipoaspirate Counterpart
AuthorDenis Polančec
AuthorLucija Zenić
AuthorDamir Hudetz
AuthorIgor Borić
AuthorŽeljko Jelec
AuthorEduard Rod
AuthorTrpimir Vrdoljak
AuthorAndrea Skelin
AuthorMihovil Plečko
AuthorMirjana Turkalj
AuthorBoro Nogalo
AuthorDragan Primorac
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Radiology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Human Genetics, Genomics and Proteomics
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Orthopedics
Abstract (english)
Osteoarthritis (OA) is a degenerative joint disease accompanied by pain and loss of function. Adipose tissue harbors mesenchymal stem/stromal cells (MSC), or medicinal signaling cells as suggested by Caplan (Caplan, 2017), used in autologous transplantation in many clinical settings. The aim of the study was to characterize a stromal vascular fraction from microfragmented lipoaspirate (SVF-MLA) applied for cartilage treatment in OA and compare it to that of autologous lipoaspirate (SVF-LA). Samples were first stained using a DuraClone SC prototype tube for the surface detection of CD31, CD34, CD45, CD73, CD90, CD105, CD146 and LIVE/DEAD Yellow Fixable Stain for dead cell detection, followed by DRAQ7 cell nuclear dye staining, and analyzed by flow cytometry. In SVF-LA and SVF-MLA samples, the following population phenotypes were identified within the CD45- fraction: CD31+CD34+CD73±CD90±CD105±CD146± endothelial progenitors (EP), CD31+CD34-CD73±CD90±CD105-CD146± mature endothelial cells, CD31-CD34-CD73±CD90+CD105-CD146+ pericytes, CD31-CD34+CD73±CD90+CD105-CD146+ transitional pericytes, and CD31-CD34+CD73highCD90+CD105-CD146- supra-adventitial-adipose stromal cells (SA-ASC). The immunophenotyping profile of SVF-MLA was dominated by a reduction of leukocytes and SA-ASC, and an increase in EP, evidencing a marked enrichment of this cell population in the course of adipose tissue microfragmentation. The role of EP in pericyte-primed MSC-mediated tissue healing, as well as the observed hormonal implication, is yet to be investigated.
Keywords (english)
Languageenglish
Publication typeScientific paper - Preliminary note / Short communication
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleGenes
Numberingvol. 10, no. 6, 474
e-ISSN2073-4425
DOIhttps://doi.org/10.3390/genes10060474
URN:NBNurn:nbn:hr:184:376119
Publication2019-06-21
Document URLhttps://www.mdpi.com/2073-4425/10/6/474
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2019-07-08 07:21:24