prikaz prve stranice dokumenta Reversible Inhibition of Murine Cytomegalovirus Replication by Gamma Interferon (IFN- ) in Primary Macrophages Involves a Primed Type I IFN-Signaling Subnetwork for Full Establishment of an Immediate-Early Antiviral State
  Download
PDF 2.24 MB
Scientific paper - Original scientific paper
Reversible Inhibition of Murine Cytomegalovirus Replication by Gamma Interferon (IFN- ) in Primary Macrophages Involves a Primed Type I IFN-Signaling Subnetwork for Full Establishment of an Immediate-Early Antiviral State
Journal of Virology, 85 (2011), 19; 10286-10299. https://doi.org/10.1128/JVI.00373-11
Kropp, Kai A.; Robertson, Kevin A.; Sing, Garwin; Rodriguez-Martin, Sara; Blanc, Mathieu; Lacaze, Paul; Hassim, Muhamad F. B. Noor Hassim; Khondoker, Mizanur R.; Busche, Andreas; Dickinson, Paul; Forster, Thorsten; Strobl, Birgit; Mueller, Mathias; Jonjić, Stipan; Angulo, Ana; Ghazal, Peter More authors...

Cite this document

Kropp, K. A., Robertson, K. A., Sing, G., Rodriguez Martin, S., Blanc, M., Lacaze, P. ... Ghazal, P. (2011). Reversible Inhibition of Murine Cytomegalovirus Replication by Gamma Interferon (IFN- ) in Primary Macrophages Involves a Primed Type I IFN-Signaling Subnetwork for Full Establishment of an Immediate-Early Antiviral State. Journal of Virology, 85. (19), 10286-10299. doi: 10.1128/JVI.00373-11

Kropp, Kai A., et al. "Reversible Inhibition of Murine Cytomegalovirus Replication by Gamma Interferon (IFN- ) in Primary Macrophages Involves a Primed Type I IFN-Signaling Subnetwork for Full Establishment of an Immediate-Early Antiviral State." Journal of Virology, vol. 85, no. 19, 2011, pp. 10286-10299. https://doi.org/10.1128/JVI.00373-11

Kropp, Kai A., Kevin A. Robertson, Garwin Sing, Sara Rodriguez Martin, Mathieu Blanc, Paul Lacaze, Muhamad F. B. Noor Hassim Hassim, et al. "Reversible Inhibition of Murine Cytomegalovirus Replication by Gamma Interferon (IFN- ) in Primary Macrophages Involves a Primed Type I IFN-Signaling Subnetwork for Full Establishment of an Immediate-Early Antiviral State." Journal of Virology 85, no. 19 (2011): 10286-10299. https://doi.org/10.1128/JVI.00373-11

Kropp, K. A., et al. (2011) 'Reversible Inhibition of Murine Cytomegalovirus Replication by Gamma Interferon (IFN- ) in Primary Macrophages Involves a Primed Type I IFN-Signaling Subnetwork for Full Establishment of an Immediate-Early Antiviral State', Journal of Virology, 85(19), pp. 10286-10299. doi: 10.1128/JVI.00373-11

Kropp KA, Robertson KA, Sing G, Rodriguez Martin S, Blanc M, Lacaze P, and sur.. Reversible Inhibition of Murine Cytomegalovirus Replication by Gamma Interferon (IFN- ) in Primary Macrophages Involves a Primed Type I IFN-Signaling Subnetwork for Full Establishment of an Immediate-Early Antiviral State. Journal of Virology [Internet]. 2011 July 20 [cited 2024 September 20];85(19):10286-10299. doi: 10.1128/JVI.00373-11

K. A. Kropp, et al., "Reversible Inhibition of Murine Cytomegalovirus Replication by Gamma Interferon (IFN- ) in Primary Macrophages Involves a Primed Type I IFN-Signaling Subnetwork for Full Establishment of an Immediate-Early Antiviral State", Journal of Virology, vol. 85, no. 19, pp. 10286-10299, July 2011. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:796831. [Accessed: 20 September 2024]

Please login to the repository to save this object to your list.
Metadata
Title (english)Reversible Inhibition of Murine Cytomegalovirus Replication by Gamma Interferon (IFN- ) in Primary Macrophages Involves a Primed Type I IFN-Signaling Subnetwork for Full Establishment of an Immediate-Early Antiviral State
AuthorKai A. Kropp
AuthorKevin A. Robertson
AuthorGarwin Sing
AuthorSara Rodriguez-Martin
AuthorMathieu Blanc
AuthorPaul Lacaze
AuthorMuhamad F. B. Noor Hassim Hassim
AuthorMizanur R. Khondoker
AuthorAndreas Busche
AuthorPaul Dickinson
AuthorThorsten Forster
AuthorBirgit Strobl
AuthorMathias Mueller
AuthorStipan Jonjić
AuthorAna Angulo
AuthorPeter Ghazal
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Histology and Embryology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
Activated macrophages play a central role in controlling inflammatory responses to infection and are tightly regulated to rapidly mount responses to infectious challenge. Type I interferon (alpha/beta interferon [IFN-α/β]) and type II interferon (IFN-γ) play a crucial role in activating macrophages and subsequently restricting viral infections. Both types of IFNs signal through related but distinct signaling pathways, inducing a vast number of interferon-stimulated genes that are overlapping but distinguishable. The exact mechanism by which IFNs, particularly IFN-γ, inhibit DNA viruses such as cytomegalovirus (CMV) is still not fully understood. Here, we investigate the antiviral state developed in macrophages upon reversible inhibition of murine CMV by IFN-γ. On the basis of molecular profiling of the reversible inhibition, we identify a significant contribution of a restricted type I IFN subnetwork linked with IFN-γ activation. Genetic knockout of the type I-signaling pathway, in the context of IFN-γ stimulation, revealed an essential requirement for a primed type I-signaling process in developing a full refractory state in macrophages. A minimal transient induction of IFN-β upon macrophage activation with IFN-γ is also detectable. In dose and kinetic viral replication inhibition experiments with IFN-γ, the establishment of an antiviral effect is demonstrated to occur within the first hours of infection. We show that the inhibitory mechanisms at these very early times involve a blockade of the viral major immediate-early promoter activity. Altogether our results show that a primed type I IFN subnetwork contributes to an immediate-early antiviral state induced by type II IFN activation of macrophages, with a potential further amplification loop contributed by transient induction of IFN-β.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleJournal of Virology
Numberingvol. 85, no. 19, pp. 10286-10299
p-ISSN0022-538X
DOIhttps://doi.org/10.1128/JVI.00373-11
URN:NBNurn:nbn:hr:184:796831
Publication2011-07-20
Publication2011-10-01
ProjectNumber: 062-0621261-1263
Title (croatian): Molekularni mehanizmi citomegalovirusnog izmicanja imunološkom nadzoru
Leader: Stipan Jonjić
Jurisdiction: Croatia
Funding stream: ZP
Document URLhttp://jvi.asm.org/cgi/doi/10.1128/JVI.00373-11
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) icon
Created on2018-11-09 08:22:41