Title Prisutnost Legionella pneumophila u bazenskoj vodi na području Primorsko-goranske županije
Title (english) Incidence of Legionella pneumophila in pool water of Primorje-Gorski Kotar County
Author Snježana Šimeg
Mentor Darija Vukić Lušić (mentor)
Committee member Željko Linšak (predsjednik povjerenstva)
Committee member Darija Vukić Lušić (član povjerenstva)
Committee member Dražen Lušić (član povjerenstva)
Granter University of Rijeka Faculty of Medicine (Department of Environmental Medicine) Rijeka
Defense date and country 2017-09-29, Croatia
Scientific / art field, discipline and subdiscipline BIOMEDICINE AND HEALTHCARE Public Health and Health Care Health Ecology
Abstract Bakterija L. pneumophila iz roda Legionella, ubikvitaran je mikroorganizam prirodno prisutan u vodi i vlažnom tlu. Opasnost za čovjeka predstavljaju legionele prisutne u umjetnim vodenim sustavima, gdje u nepovoljnim uvjetima može perzistirati i do godine dana. U toplim i vlažnim uvjetima, poput različitih vrsta bazena (posebice onih s temperaturom vode u rasponu 20 °C – 45 °C) ova bakterija može nesmetano preživljavati i razmnožavati se više mjeseci. Put zaraze predstavlja inhalacija aerosola ili mikroaspiracija kontaminirane vode. Povećani rizik za razvoj epidemije javlja se nakon dužeg nekorištenja objekta, a jedan od primjera takve prakse rada su bazenska kupališta sa sezonskim režimom rada.
Cilj ovog rada bio je prikazati mikrobiološku kakvoću bazena na području Primorsko-goranske županije s aspekta prisutnosti bakterije L. pneumophila. Analizirani su podaci prisutnosti bakterije, te povezanost s dodatnim fizikalno-kemijskim i mikrobiološkim pokazateljima kakvoće bazenske vode, u četverogodišnjem razdoblju ispitivanja (2013. – 2016.). Rezultati ukazuju na generalno nizak udio (2 %) pozitivnih rezultata na pokazatelj L. pneumophila, međutim s trendom porasta, što odgovara porastu udjela spa bazena u ukupnom broju analiziranih uzoraka. Općenito, broj svih ispitanih uzoraka raste, a najveći je u glavnim turističkim centrima (Opatija, Krk, Rab). Veći udio pozitivnih uzoraka javlja se kod slatke vode.U pozitivnim je uzorcima, u odnosu na uzorke u kojima L. pneumophila nije dokazana, zabilježena niža koncentracija rezidualnog klora i povišeni broj UBB, međutim razlika se nije pokazala statistički značajnom. T-test je između pozitivnih i negativnih uzoraka pokazao statistički značajnu razliku jedino u pokazatelju temperaturi vode, s značajno višim vrijednostima kod pozitivnih uzoraka. Obzirom da razlika u razinama klora između pozitivnih i negativnih uzoraka nije statistički značajna, upitna je efikasnost djelovanja klora na legionele unutar vodenog sustava. Naime, prema rezultatima legionele se mogu dokazati i pri višim koncentracijama klora.
Nakon detaljnije analize rezultata ispitivanja u četverogodišnjem razdoblju zaključujemo da za praćenje pojavnosti L. pneumophila u vodi presudnu ulogu može imati izbor adekvatne metode ispitivanja, čemu je potrebno posvetiti posebnu pažnju.Također, u budućnosti je potrebno definirati ispitivanje prisutnosti L. pneumophila u vodi za piće iz vodoopskrbnih sustava, što se trenutno provodi u slučajevima epidemiološke indikacije
Abstract (english) in water and wet soil. Dangers to man represent legionnaires present in artificial water systems, where baceria can persist in unfavorable conditions for up to a year. In warm and humid conditions, such as one present in various pool types (especially those with water temperature ranging from 20 °C to 45 °C), this bacterium can undisturbed survive and multiply for several months. The infection is resault of contact with contaminated water by aerosol inhalation or micro-aspiration. An increased risk of developing an epidemic occurs after a long not used period of the facility, and one of the examples of such practice is swimming pool with seasonal work regimen.
The aim of this study was to show the microbiological quality of the pool in the area of the Primorje-Gorski Kotar County from the aspect of the presence of L. pneumophila. Data on the presence of this bacteria, as well as correlation with additional physico-chemical and microbiological basin water quality indicators, were analyzed in the four-year study period (2013 - 2016). The results indicate a generally low part (2%) of positive results of the L. pneumophila indicator, however, with the growth trend, that corresponding to the increase in spa pool part in the total number of analyzed samples. Generally, the number of all tested samples is growing, and is the largest in major tourist centers (Opatija, Krk, Rab). A higher part of positive samples occurs in fresh water. In positive samples, in regards to samples in which L. pneumophila was not shown, lower residual chlorine concentration and elevated UBB levels were observed, however the difference was not statistically significant. The T-test between positive and negative samples showed statistically significant difference only in the water temperature indicator, with significantly higher values in positive samples. Since the difference in the chlorine levels between the positive and negative samples is not statistically significant, the efficiency of the chlorine action on the legionnaires within the aquatic system is questionable. Namely, legionnaires can be proved at higher chlorine concentrations.
After a more detailed analysis of the results of the four-year study, we conclude that in order to monitor the occurrence of L. pneumophila in water, the choice of the appropriate test method can be a crucial part of the study, and special attention should be paid to this. Also, in the future it is necessary to define the presence of L. pneumophila in drinking water from water supply systems, which is currently being carried out only in the case of epidemiological indications
Keywords
L. pneumophila
bazeni
spa
metoda ispitivanja
membranska filtracija
Keywords (english)
L. pneumophila
pools
spa
test method
membrane filtration
Language croatian
URN:NBN urn:nbn:hr:184:372323
Study programme Title: Study of Sanitary Engineering Study programme type: university Study level: graduate Academic / professional title: magistar/magistra sanitarnog inženjerstva (magistar/magistra sanitarnog inženjerstva)
Type of resource Text
File origin Born digital
Access conditions Open access
Terms of use
Created on 2018-06-20 07:01:26