Iron is an essential nutrient for nearly every living organism, is essential in a number of biological processes that serve to sustain life. In addition to iron, copper and zinc have an important role as well as essential nutrients. These metals are cationic trace elements, although each of these elements has a specific function and metabolic regulation, together they make an mesh network of related functions. They are transmitted by specific carriers and participate in a variety of reactions in the body. Many enzymes in its structure contain zinc and copper which contribute to the catalytic activity of the enzyme. Copper and iron, due to the possibility of electron transfer are basis of oxidation-reduction processes, such as respiration processes. In case of increased concentration and disturbed distribution in the body, can cause intoxication and lead to various pathological conditions. Iron overload is one of the most common iron intoxication, can cause oxidative stress, trough formation of free radicals. Copper, zinc and iron share individual carriers which in the case of pathological changes in their levels in the body can lead to competitive interactions for certain transporter and errors in the metabolism of these metals. The aim of this study is to show how an increased intake of iron status affects on concentration of the elements copper and zinc in the liver in the rat model of experimental autoimmune encephalomyelitis (EAE), which is an animal model for multiple sclerosis (MS), demyelinating autoimmune, neurodegenerative disease in humans. In experiment, we used the Dark Agouti (DA) rats of male sex, which were divided into three groups: an experimental group, treated with iron, the control group, treated with saline solution and untreated group. Samples of liver tissue processed by the method of microwave digestion. The concentrations of zinc and copper in the liver tissue were determined using ICP-OES method on Prodigy High Dispersion ICP-OES spectrophotometer with pneumatic nebulizer. Average values of the measured concentrations of zinc and copper in the liver tissue of rats in a model of EAE were not significantly changed in the experimental group treated with iron as compared to the control group treated with saline and an untreated group of rats.