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master's thesis
Učinci pioglitazona u traumatskoj ozljedi mozga u štakora
2019. urn:nbn:hr:184:027712
Slavić, Ante
University of Rijeka
Faculty of Medicine
Department of Pharmacology

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Slavić, A. (2019). Učinci pioglitazona u traumatskoj ozljedi mozga u štakora (Master's thesis). Rijeka: University of Rijeka, Faculty of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:184:027712

Slavić, Ante. "Učinci pioglitazona u traumatskoj ozljedi mozga u štakora." Master's thesis, University of Rijeka, Faculty of Medicine, 2019. https://urn.nsk.hr/urn:nbn:hr:184:027712

Slavić, Ante. "Učinci pioglitazona u traumatskoj ozljedi mozga u štakora." Master's thesis, University of Rijeka, Faculty of Medicine, 2019. https://urn.nsk.hr/urn:nbn:hr:184:027712

Slavić, A. (2019). 'Učinci pioglitazona u traumatskoj ozljedi mozga u štakora', Master's thesis, University of Rijeka, Faculty of Medicine, accessed 24 November 2024, https://urn.nsk.hr/urn:nbn:hr:184:027712

Slavić A. Učinci pioglitazona u traumatskoj ozljedi mozga u štakora [Master's thesis]. Rijeka: University of Rijeka, Faculty of Medicine; 2019 [cited 2024 November 24] Available at: https://urn.nsk.hr/urn:nbn:hr:184:027712

A. Slavić, "Učinci pioglitazona u traumatskoj ozljedi mozga u štakora", Master's thesis, University of Rijeka, Faculty of Medicine, Rijeka, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:184:027712

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Metadata
TitleUčinci pioglitazona u traumatskoj ozljedi mozga u štakora
Title (english)Effects of pioglitazone in the traumatic brain injury in the rat
AuthorAnte Slavić
MentorKristina Pilipović (mentor)
Committee memberJasenka Mršić-Pelčić (predsjednik povjerenstva)
Committee memberKristina Pilipović (član povjerenstva)
Committee memberTanja Ćelić (član povjerenstva)
GranterUniversity of Rijeka
Faculty of Medicine
(Department of Pharmacology)
Rijeka
Defense date and country2019-09-24, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Pharmacology
Abstract
U današnje vrijeme traumatska ozljeda mozga (TBI) jedan je od glavnih socioekonomskih i javnozdravstvenih problema. Zbog svoje velike smrtnosti i brojnih poteškoća nastalih od same ozljede, TBI je potrebno pridati više pažnje. U ovom radu je opisan utjecaj primjene pioglitazona na neurodegeneraciju i ekspresiju mikroglije u parijetalnom korteksu i hipokampusu te utjecaj na ekspresiju ciklooksigenaze-2 (COX-2), inducibilne oksidaze dušikovog oksida (iNOS) i proteina toplinskog šoka 70 (HSP70) u hipokampusu nakon TBI uzrokovane modelom lateralne ozljede tlakom tekućine (LPFI). Pioglitazon je primijenjen u dozi od 1 mg/kg 10 min i 12 h nakon indukcije TBI. Kontrolna grupa štakora je lažno operirana i primijenjeno im je otapalo. Štakori su dekapitirani 24 sata nakon TBI te su parijetalni korteks i hipokampus analizirani biokemijskim i histološkim metodama. Analize su pokazale da nakon TBI nastaju neurodegenerativne promjene u parijetalnom korteksu i dentatnom girusu (DG) hipokampusa, kao i značajna reakcija mikroglije u parijetalnom korteksu i svim ispitivanim dijelovima hipokampusa. U hipokampusu je zabilježena i povećana ekspresija COX-2 i HSP70. Pioglitazon je značajno smanjio broj Iba1 pozitivnih stanica u DG hipokampusa ozlijeđenih životinja, dok na ostale ispitvane parametre nije imao učinka. Ovim radom pokazano je da pioglitazon, primijenjen u dvije doze nakon TBI uzrokovane s LPFI, djeluje na posttraumastku reaktivnu mikrogliozu u određenim moždanim regijama, no točan mehanizam ovog djelovanja nije jasan te su potrebna daljnja istraživanja učinaka ovog lijeka u tretmanu TBI.
Abstract (english)
Today, traumatic brain injury (TBI) is one of the major socio-economic and public health issues. Due to its high mortality and the many difficulties caused by the injury itself, TBI needs more attention. This paper describes the effects of pioglitazone on the neurodegeneration and expression of microglia in the parietal cortex and hippocampus, and the effect on the expression of COX-2, iNOS, and HSP70 in the hippocampus, after TBI caused by lateral fluid pressure injury (LPFI) model. Pioglitazone was administered at a dose of 1 mg/kg 10 min and 12 h after TBI induction. The control group of rats was sham operated and the vehicle was administered instead of the drug. Rats were decapitated 24 hours after TBI and the parietal cortex and hippocampus were analyzed by biochemical and histological methods. Analyzes revealed that after TBI, neurodegeneration occurs in the parietal cortex and dentate gyrus (DG) of the hippocampus, as well as microglia reactions in the parietal cortex and all the tested areas of the hippocampus. Expressions of COX-2 and HSP70 in the hippocampus were significantly higher after brain trauma. Pioglitazone cause a reduction in the number of Iba1 positive cells in the DG hippocampus of the injured animals compared to vehicle-treated, injured rats. This work demonstrates that pioglitazone, administered at two doses after LPFI-induced TBI, can significantly reduce the number of Iba1 positive cells in certain brain regions, but the exact mechanism of this effect is not clear suggesting that further studies on this drug in the treatment of TBI are needed.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:184:027712
Study programmeTitle: Study of Sanitary Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra sanitarnog inženjerstva (magistar/magistra sanitarnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2020-01-27 08:49:25