Introduction: Hemp, lat. Cannabis sativa L. is a one year, dioecious, aromatic plant with a recognizable flower and cannabinoids. Cannabis sativa L. subspecies are C. sativa L. spp. sativa, cannabidiol (CBD) - hemp and C. sativa L. spp. indica, Δ9-tetrahydrocannabinol (THC) - hemp. Cannabinoids are lipophilic molecules accumulated as cannabinoid acids in the hemp. On the microscopic level, there are two types of trichomes in the hemp but glandular trichomes are the ones that are rich with cannabinoids. Cannabinoids are extracted with organic solvents from the sample, and analyzed by high performance liquid chromatography (HPLC). Aim of the study: Identification of the hemp is done by using a microscope. Than, the qualitative and quantitative composition of cannabinoids in extracted samples of hemp was assesed by using different solvents, sieving, mixing and extraction dependent on the homogenization time with solvent. Materials and methods: Hemp samples for experimental work were obtained from the archives of the Faculty of Pharmacy, University of Ljubljana. The experiment involves the extraction with different solvents, sieving, mixing and extraction dependent on the homogenization time with solvent. Sample analysis was performed on HPLC system (Shimadzu Corporation, Japan). Results and Discussion: Microscopy can established the identification of hemp by the presence of characteristic trihomes. Different composition of cannabinoids is present in different samples. There is no statistically significant differences between ekxtraction with diffrent solvents but the highest concentration of cannabinoids was determined after extraction with ethanol. After sieving, there is increased amount of the cannabinoids. Larger amounts of cannabinoids are obtained in unmixed samples, and mixed samples have better representativity. Vortexing resulted in a higher amount of cannabinoid but ultrasound resulted in higher amount of the extract. Conclusions: Hemp was positively identified by microscopy. The qualitative and quantitative composition of cannabinoids in different samples depends on the type of samples. Ethanol, methanol and methanol:chloroform (9:1) equally dissolve cannabinoids. To obtain a larger quantity of cannabinoids, a sample should be sieved, and to obtain a better representative sample, a sample should be mixed. To obtain higher concentrations of cannabinoids in extraction, it is best to combine vortication and ultrasound