ABSTRACT. Aim: The role of PLA2 superfamily enzymes is indicated in the human testes, associated with disturbance of spermatogenesis, and with hormonal secretion from anterior pituitary. The aim of this study was to investigate the association between two polymorphisms (rs10798059 in the cytosolic PLA2 gene -PLA2G4A and rs4375 in the calcium-independent PLA2 gene-PLA2G6A) and clinical features of 96 oligoasthenospermic or asthenospermic men. Methods: Sixty five infertile men with idiopathic infertility were included in the study. Genetic analyses were performed using PCR-RFLP method while chi-square test, multiple stepwise regression and ANOVA were used in the statistical analyses. Results: The frequencies of cPLA2 and iPLA2 genotypes and alleles were not significantly different between tested group and 59 control men. Multiple stepwise regression analysis showed a statistically significant association between both investigated polymorphisms and FSH serum level in 96 patients (P<0,05). Furthermore, iPLA2 polymorphism was significantly associated with sperm number and total serum testosterone level (in both cases P<0,05). Additional multiple stepwise regression analysis in 65 men with idiopathic infertility confirmed an association between iPLA2 (βFSH=0,504; P<0,0001) and cPLA2 (βFSH=0,322; P<0,01) polymorphisms and FSH means at a higher level of statistical significance. The same analysis also revealed an association between LH means and iPLA2 polymorphism (βLH=0,319; P<0,02). The genotype C/C of the iPLA2 polymorphism was associated with the highest mean FSH value (above the physiologic range) while the lowest mean was found in heterozygotes (T/C). According to iPLA2 genotype, LH means varied within the physiologic range but were distributed in the same manner as did FSH means. Conclusions: The results suggest that investigated iPLA2 and cPLA2 polymorphisms might influence the exocytosis of gonadotropins from the anterior pituitary and/or testes function, thus modulating reproductive fitness in men.