Due to an excesive use, neonicotinoid insecticides show a toxic effect on ecosystems and living beings in the environment. In Croatia, they are often used in Slavonia as an effective means to protect sugar beet plantations or in coastal regions to protect olive groves from olive flies. Neonicotinoids work by binding non-selectively to the acetylcholine receptors on the CNS of all insects, which can have long-term consequences for the ecosystem in which it is applied. One ecosystem often exposed to the negative effects of neonicotinoids is soil. The behaviour of insecticides in soil depends on the characteristics of the soil, primarily the amount of organic matter (SOM), but also on the chemical structure of the insecticides themselves. The goal of this research is to examine the influence of different soil characteristics on the intensity of binding of acetamiprid, tiamethoxam and clothianidin to soils from the Republic of Croatia, and to analyze their sorption/desorption processes. The data analyzed is best represented by the Freundlich model. . In the case of desorption processes, the KF values for all analyzed insecticides are significantly higher compared to the sorption KF values. This research confirms that the sorption-desorption processes of neonicotinoids are statistically dependent on the organic phase of the soil. The values of the nonlinearity factor differed considerably between the desorption and sorption processes, which indicated a discrepancy between the sorption and desorption isotherms, this is the occurrence of the so called "hysteresis" phenomena. The mean value of KOC coefficients in this research classifies the analyzed neonicotinoids as highly mobile micropollutants. The low Gibbs energy of sorption and desorption processes indicates that the sorption and desorption processes of analyzed neonicotinoids take place through physical processes, the primary mechanism of which is the distribution of insecticides within organic matter of the soil. The results of the research emphasize that the controlled application of the analyzed neonicotinoids is mandatory, especially in soils with a low content of organic substances, in order to minimize the risk of environmental and groundwater pollution..