Aim: The aim of the study was to examine students’ awareness of testicular tumors, their knowledge of self-examination, and motivation to learn more about the disease. Subjects and Methods: The survey was conducted among 116 students of the University of Rijeka. The first group (42 students) consisted of first year students of the Department of Informatics, the second group consisted of 44 students of the second year of the Department of Informatics and the third group consisted of 30 young men of other student population. The survey used an anonymous survey questionnaire with 18 questions. Results: The median age of the participants in the first and third group was 19 years and 20 years in the second group. The largest number of students came from Primorje-Gorski Kotar County. The information about testicular tumor was known in 71.4% of students in the first group, 93.2% of students in the second group and 90% of students in the third group. The most important source of information for all three groups is the Internet and television. The testicular self-examination was done by 57.8% of the participants, and only 45.7% knew how to do it properly. Conclusions: Students are familiar with testicular tumors via the Internet and television and are eager to be better informed. Testicular self-examination, the method that can detect this disease, is conducted by only 50% of students, and less than 50% know how to do it, which is unsatisfactory. The data of our study show that it is necessary to educate students about this health problem.