prikaz prve stranice dokumenta BIG, GBF AND BRAG ARF GEFS ARE REQUIRED FOR THE PROGRESSION OF IMMEDIATE-EARLY AND EARLY PHASE OF MCMV INFECTION
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BIG, GBF AND BRAG ARF GEFS ARE REQUIRED FOR THE PROGRESSION OF IMMEDIATE-EARLY AND EARLY PHASE OF MCMV INFECTION
Na: Congress of the Croatian Society of Biochemistry and Molecular Biology HDBMB22: From Science to Knowledge. Brela, Hrvatska, 2022. urn:nbn:hr:184:999161
Blagojević Zagorac, Gordana; Karleuša, Ljerka; Viduka, Ivona; Jug Vučko, Natalia; Pavišić, Valentino; Mahmutefendić Lučin, Hana; Lučin, Pero

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Blagojević Zagorac, G., Karleuša, Lj., Viduka, I., Jug Vučko, N., Pavišić, V., Mahmutefendić Lučin, H. i Lučin, P. BIG, GBF AND BRAG ARF GEFS ARE REQUIRED FOR THE PROGRESSION OF IMMEDIATE-EARLY AND EARLY PHASE OF MCMV INFECTION [PowerPoint prezentacija]. Preuzeto s https://urn.nsk.hr/urn:nbn:hr:184:999161

Blagojević Zagorac, Gordana, et al. "BIG, GBF AND BRAG ARF GEFS ARE REQUIRED FOR THE PROGRESSION OF IMMEDIATE-EARLY AND EARLY PHASE OF MCMV INFECTION." Congress of the Croatian Society of Biochemistry and Molecular Biology HDBMB22: From Science to Knowledge, 28.09.2022.-01.10.2022., Brela, Hrvatska. Prezentacija. https://urn.nsk.hr/urn:nbn:hr:184:999161

Blagojević Zagorac, Gordana, Ljerka Karleuša, Ivona Viduka, Natalia Jug Vučko, Valentino Pavišić, Hana Mahmutefendić Lučin i Pero Lučin. "BIG, GBF AND BRAG ARF GEFS ARE REQUIRED FOR THE PROGRESSION OF IMMEDIATE-EARLY AND EARLY PHASE OF MCMV INFECTION." Prezentacija sa skupa Congress of the Croatian Society of Biochemistry and Molecular Biology HDBMB22: From Science to Knowledge, Brela, Hrvatska, 28.09.2022.-01.10.2022. https://urn.nsk.hr/urn:nbn:hr:184:999161

Blagojević Zagorac, G., et al. 'BIG, GBF AND BRAG ARF GEFS ARE REQUIRED FOR THE PROGRESSION OF IMMEDIATE-EARLY AND EARLY PHASE OF MCMV INFECTION' [Prezentacija], Congress of the Croatian Society of Biochemistry and Molecular Biology HDBMB22: From Science to Knowledge. 28.09.2022.-01.10.2022. Preuzeto s: https://urn.nsk.hr/urn:nbn:hr:184:999161

Blagojević Zagorac G, Karleuša Lj, Viduka I, Jug Vučko N, Pavišić V, Mahmutefendić Lučin H, i sur.. BIG, GBF AND BRAG ARF GEFS ARE REQUIRED FOR THE PROGRESSION OF IMMEDIATE-EARLY AND EARLY PHASE OF MCMV INFECTION. [Prezentacija] [pristupljeno 04.12.2024.] Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:999161

G. Blagojević Zagorac, et al., "BIG, GBF AND BRAG ARF GEFS ARE REQUIRED FOR THE PROGRESSION OF IMMEDIATE-EARLY AND EARLY PHASE OF MCMV INFECTION", prezentirano na: Congress of the Croatian Society of Biochemistry and Molecular Biology HDBMB22: From Science to Knowledge, Brela, 28.09.2022.-01.10.2022. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:999161

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Naslov (engleski)BIG, GBF AND BRAG ARF GEFS ARE REQUIRED FOR THE PROGRESSION OF IMMEDIATE-EARLY AND EARLY PHASE OF MCMV INFECTION
AutorGordana Blagojević Zagorac
AutorLjerka Karleuša
AutorIvona Viduka
AutorNatalia Jug Vučko
AutorValentino Pavišić
AutorHana Mahmutefendić Lučin
AutorPero Lučin
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za fiziologiju, imunologiju i patofiziologiju)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Sažetak (engleski)
Arf proteins are small GTPases that cycle between active (GTP) and inactive (GDP) forms. Their activation is mediated by guanine nucleotide exchange factors (GEFs), and their inactivation is facilitated by GTPase-activating proteins (GAPs). Since we have previously shown that Arf1 and Arf6 proteins are required for the establishment of murine cytomegalovirus (MCMV) infection, the aim of this study was to determine importance of Arf GEFs in regulation of Arf´s activity during the immediate-early and early phase of MCMV infection. Balb 3T3 murine fibroblasts treated with small inhibitors of Arf GEFs were infected with MCMV and the expression of viral proteins was determined by Western blot and immunofluorescence microscopy after 6 and 16 hours of infection. In addition, cells were infected with C3X-GFP MCMV, in which a GFP expression cassette was inserted upstream of the ie2 gene, and the intensity of green fluorescence as well as percentage of GFP positive cells was monitored by flow cytometry as an indicators for progression of the immediate-early phase of infection. The expression of IE1, E1 and m06 proteins was reduced in cells treated with Brefeldin A (BFA), Golgicid A (GCA), NAV2729 or EXO2, in contrast to cells treated with SecinH3 or EXO1 in which the expression of the viral proteins was similar to that in control cells. In cells infected with C3X-GFP MCMV, percentage of GFP positive cells and the green fluorescence intensity were significantly decreased in cells treated with BFA, GCA and EXO2 compared with control cells, suggesting that inhibition of Gbf1, Big1, Big2 and BRAG2 Arf GEFs prevents entry into the immediate–early/early phase of MCMV gene expression. Our results suggest that Arf GEFs from Big, Gbf and Brag2 family have important role in regulation of Arf protein activity during the earliest stages of MCMV infection and are required for the progression of immediate-early and early phase of MCMV infection.
Jezikengleski
Vrsta recenzijeNepoznato
URN:NBNurn:nbn:hr:184:999161
Datum izlaganja2022-10-01
ProjektŠifra: IP-2020-02-1323
Naziv (hrvatski): Stanični sustav Arf GTPaza u patogenezi citomegalovirusne infekcije
Voditelj: Gordana Blagojević zagorac
Pravna nadležnost: Hrvatska
Financijer: HRZZ
ProjektŠifra: IP-2019-04-3582
Naziv (hrvatski): Sekundarno omatanje i izlazak beta-herpesvirusa iz stanice
Voditelj: Pero Lučin
Pravna nadležnost: Hrvatska
Financijer: HRZZ
SkupNaziv: Congress of the Croatian Society of Biochemistry and Molecular Biology HDBMB22: From Science to Knowledge
Lokacija: Brela, Hrvatska
Datum početka: 2022-09-28
Datum završetka: 2022-10-01
Vrsta izlaganja: Predavanje
Vrsta resursaTekst
Prava pristupaOgraničen pristup
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Datum i vrijeme pohrane2023-04-05 11:52:52