prikaz prve stranice dokumenta Real-Time PCR Quantification of 87 miRNAs from Cerebrospinal Fluid: miRNA Dynamics and Association with Extracellular Vesicles after Severe Traumatic Brain Injury
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Real-Time PCR Quantification of 87 miRNAs from Cerebrospinal Fluid: miRNA Dynamics and Association with Extracellular Vesicles after Severe Traumatic Brain Injury
International Journal of Molecular Sciences, 24 (2023), 5; 4751. https://doi.org/10.3390/ijms24054751
Valenčić Seršić, Lara; Krušić Alić, Vedrana; Biberić, Maša; Zrna, Siniša; Jagoić, Tin; Tarčuković, Janja; Grabušić, Kristina

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Valenčić Seršić, L., Krušić Alić, V., Biberić, M., Zrna, S., Jagoić, T., Tarčuković, J. i Grabušić, K. (2023). Real-Time PCR Quantification of 87 miRNAs from Cerebrospinal Fluid: miRNA Dynamics and Association with Extracellular Vesicles after Severe Traumatic Brain Injury. International Journal of Molecular Sciences, 24. (5). doi: 10.3390/ijms24054751

Valenčić Seršić, Lara, et al. "Real-Time PCR Quantification of 87 miRNAs from Cerebrospinal Fluid: miRNA Dynamics and Association with Extracellular Vesicles after Severe Traumatic Brain Injury." International Journal of Molecular Sciences, vol. 24, br. 5, 2023. https://doi.org/10.3390/ijms24054751

Valenčić Seršić, Lara, Vedrana Krušić Alić, Maša Biberić, Siniša Zrna, Tin Jagoić, Janja Tarčuković i Kristina Grabušić. "Real-Time PCR Quantification of 87 miRNAs from Cerebrospinal Fluid: miRNA Dynamics and Association with Extracellular Vesicles after Severe Traumatic Brain Injury." International Journal of Molecular Sciences 24, br. 5 (2023). https://doi.org/10.3390/ijms24054751

Valenčić Seršić, L., et al. (2023) 'Real-Time PCR Quantification of 87 miRNAs from Cerebrospinal Fluid: miRNA Dynamics and Association with Extracellular Vesicles after Severe Traumatic Brain Injury', International Journal of Molecular Sciences, 24(5). doi: 10.3390/ijms24054751

Valenčić Seršić L, Krušić Alić V, Biberić M, Zrna S, Jagoić T, Tarčuković J, i sur.. Real-Time PCR Quantification of 87 miRNAs from Cerebrospinal Fluid: miRNA Dynamics and Association with Extracellular Vesicles after Severe Traumatic Brain Injury. International Journal of Molecular Sciences [Internet]. 01.03.2023. [pristupljeno 05.05.2024.];24(5). doi: 10.3390/ijms24054751

L. Valenčić Seršić, et al., "Real-Time PCR Quantification of 87 miRNAs from Cerebrospinal Fluid: miRNA Dynamics and Association with Extracellular Vesicles after Severe Traumatic Brain Injury", International Journal of Molecular Sciences, vol. 24, br. 5, Ožujak 2023. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:881493. [Citirano: 05.05.2024.]

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Naslov (engleski)Real-Time PCR Quantification of 87 miRNAs from Cerebrospinal Fluid: miRNA Dynamics and Association with Extracellular Vesicles after Severe Traumatic Brain Injury
AutorLara Valenčić Seršić
AutorVedrana Krušić Alić
AutorMaša Biberić
AutorSiniša Zrna
AutorTin Jagoić
AutorJanja Tarčuković
AutorKristina Grabušić
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za anesteziologiju, reanimatologiju, hitnu i intenzivnu medicinu)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Kliničke medicinske znanosti
Anesteziologija i reanimatologija
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Kliničke medicinske znanosti
Intenzivna medicina
Sažetak (engleski)
Severe traumatic brain injury (sTBI) is an intracranial damage triggered by external force, most commonly due to falls and traffic accidents. The initial brain injury can progress into a secondary injury involving numerous pathophysiological processes. The resulting sTBI dynamics makes the treatment challenging and prompts the improved understanding of underlying intracranial processes. Here, we analysed how extracellular microRNAs (miRNAs) are affected by sTBI. We collected thirty-five cerebrospinal fluids (CSF) from five sTBI patients during twelve days (d) after the injury and combined them into d1–2, d3–4, d5–6 and d7–12 CSF pools. After miRNA isolation and cDNA synthesis with added quantification spike-ins, we applied a real-time PCR-array targeting 87 miRNAs. We detected all of the targeted miRNAs, with totals ranging from several nanograms to less than a femtogram, with the highest levels found at d1–2 followed by decreasing levels in later CSF pools. The most abundant miRNAs were miR-451a, miR-16-5p, miR-144-3p, miR-20a-5p, let-7b-5p, miR-15a-5p, and miR-21-5p. After separating CSF by size-exclusion chromatography, most miRNAs were associated with free proteins, while miR-142-3p, miR-204-5p, and miR-223-3p were identified as the cargo of CD81-enriched extracellular vesicles, as characterised by immunodetection and tunable resistive pulse sensing. Our results indicate that miRNAs might be informative about both brain tissue damage and recovery after sTBI.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaInternational Journal of Molecular Sciences
Brojčani podacivol. 24, br. 5, 4751
e-ISSN1422-0067
DOIhttps://doi.org/10.3390/ijms24054751
URN:NBNurn:nbn:hr:184:881493
Datum objave publikacije2023-03-01
ProjektŠifra: IP-2019-04-1511
Naziv (hrvatski): Identifikacija cirkulirajućih biomarkera neurološkog oporavka u bolesnika s ozljedom mozga
Naziv (engleski): Identification of circulating biomarkers for neuro-recovery of brain-injured patients
Kratica: CiMozak / CirBrain
Voditelj: Kristina Grabušić
Pravna nadležnost: Hrvatska
Financijer: HRZZ
Linija financiranja: IP
ProjektŠifra: uniri-biomed-18-5
Naziv (hrvatski): Izvanstanične vezikule kao klinički markeri neuroregeneracije nakon teške ozljede mozga
Naziv (engleski): Extracellular vesicles as clinical markers for neuroregeneration after severe traumatic brain injury
Voditelj: Kristina Grabušić
Pravna nadležnost: Hrvatska
Financijer: NadSve
Linija financiranja: Sveučilište u Rijeci
URL dokumentahttps://www.mdpi.com/1422-0067/24/5/4751
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 4.0 međunarodna (CC BY 4.0)
Datum i vrijeme pohrane2023-03-03 07:00:35