prikaz prve stranice dokumenta Extracellular Vesicles from Human Cerebrospinal Fluid Are Effectively Separated by Sepharose CL-6B—Comparison of Four Gravity-Flow Size Exclusion Chromatography Methods
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Extracellular Vesicles from Human Cerebrospinal Fluid Are Effectively Separated by Sepharose CL-6B—Comparison of Four Gravity-Flow Size Exclusion Chromatography Methods
Biomedicines, 10 (2022), 4; 785. https://doi.org/10.3390/biomedicines10040785
Krušić Alić, Vedrana; Malenica, Mladenka; Biberić, Maša; Zrna, Siniša; Valenčić, Lara; Šuput, Aleksandar; Kalagac Fabris, Lada; Wechtersbach, Karmen; Kojc, Nika; Kurtjak, Mario; Kučić, Natalia; Grabušić, Kristina

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Krušić Alić, V., Malenica, M., Biberić, M., Zrna, S., Valenčić, L., Šuput, A. ... Grabušić, K. (2022). Extracellular Vesicles from Human Cerebrospinal Fluid Are Effectively Separated by Sepharose CL-6B—Comparison of Four Gravity-Flow Size Exclusion Chromatography Methods. Biomedicines, 10. (4). doi: 10.3390/biomedicines10040785

Krušić Alić, Vedrana, et al. "Extracellular Vesicles from Human Cerebrospinal Fluid Are Effectively Separated by Sepharose CL-6B—Comparison of Four Gravity-Flow Size Exclusion Chromatography Methods." Biomedicines, vol. 10, br. 4, 2022. https://doi.org/10.3390/biomedicines10040785

Krušić Alić, Vedrana, Mladenka Malenica, Maša Biberić, Siniša Zrna, Lara Valenčić, Aleksandar Šuput, Lada Kalagac Fabris, et al. "Extracellular Vesicles from Human Cerebrospinal Fluid Are Effectively Separated by Sepharose CL-6B—Comparison of Four Gravity-Flow Size Exclusion Chromatography Methods." Biomedicines 10, br. 4 (2022). https://doi.org/10.3390/biomedicines10040785

Krušić Alić, V., et al. (2022) 'Extracellular Vesicles from Human Cerebrospinal Fluid Are Effectively Separated by Sepharose CL-6B—Comparison of Four Gravity-Flow Size Exclusion Chromatography Methods', Biomedicines, 10(4). doi: 10.3390/biomedicines10040785

Krušić Alić V, Malenica M, Biberić M, Zrna S, Valenčić L, Šuput A, i sur.. Extracellular Vesicles from Human Cerebrospinal Fluid Are Effectively Separated by Sepharose CL-6B—Comparison of Four Gravity-Flow Size Exclusion Chromatography Methods. Biomedicines [Internet]. 27.03.2022. [pristupljeno 04.12.2024.];10(4). doi: 10.3390/biomedicines10040785

V. Krušić Alić, et al., "Extracellular Vesicles from Human Cerebrospinal Fluid Are Effectively Separated by Sepharose CL-6B—Comparison of Four Gravity-Flow Size Exclusion Chromatography Methods", Biomedicines, vol. 10, br. 4, Ožujak 2022. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:277557. [Citirano: 04.12.2024.]

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Naslov (engleski)Extracellular Vesicles from Human Cerebrospinal Fluid Are Effectively Separated by Sepharose CL-6B—Comparison of Four Gravity-Flow Size Exclusion Chromatography Methods
AutorVedrana Krušić Alić
AutorMladenka Malenica
AutorMaša Biberić
AutorSiniša Zrna
AutorLara Valenčić
AutorAleksandar Šuput
AutorLada Kalagac Fabris
AutorKarmen Wechtersbach
AutorNika Kojc
AutorMario Kurtjak
AutorNatalia Kučić
MBZ: 239812
AutorKristina Grabušić
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za medicinsku kemiju, biokemiju i kliničku kemiju)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Kliničke medicinske znanosti
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Sažetak (engleski)
Extracellular vesicles (EVs) are a versatile group of cell-secreted membranous nanoparticles present in body fluids. They have an exceptional diagnostic potential due to their molecular content matching the originating cells and accessibility from body fluids. However, methods for EV isolation are still in development, with size exclusion chromatography (SEC) emerging as a preferred method. Here we compared four types of SEC to isolate EVs from the CSF of patients with severe traumatic brain injury. A pool of nine CSF samples was separated by SEC columns packed with Sepharose CL-6B, Sephacryl S-400 or Superose 6PG and a ready-to-use qEV10/70 nm column. A total of 46 fractions were collected and analysed by slot-blot followed by Ponceau staining. Immunodetection was performed for albumin, EV markers CD9, CD81, and lipoprotein markers ApoE and ApoAI. The size and concentration of nanoparticles in fractions were determined by tunable resistive pulse sensing and EVs were visualised by transmission electron microscopy. We show that all four SEC techniques enabled separation of CSF into nanoparticle- and free protein-enriched fractions. Sepharose CL-6B resulted in a significantly higher number of separated EVs while lipoproteins were eluted together with free proteins. Our data indicate that Sepharose CL-6B is suitable for isolation of EVs from CSF and their separation from lipoproteins.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaBiomedicines
Brojčani podacivol. 10, br. 4, 785
e-ISSN2227-9059
DOIhttps://doi.org/10.3390/biomedicines10040785
URN:NBNurn:nbn:hr:184:277557
Datum objave publikacije2022-03-27
URL dokumentahttps://www.mdpi.com/2227-9059/10/4/785
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 4.0 međunarodna (CC BY 4.0)
Datum i vrijeme pohrane2022-04-26 11:53:25