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The UL97 product of human cytomegalovirus is an early-late protein with a nuclear localization but is not a nucleoside kinase
Journal of Virology, 70 (1996), 9; 6340-6346. urn:nbn:hr:184:830706
Michel, Detlef; Pavić, Ivica; Zimmermann, Albert; Haupt, Edith; Wunderlich, Kirsten; Heuschmid, Maria; Mertens, Thomas

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Michel, D., Pavić, I., Zimmermann, A., Haupt, E., Wunderlich, K., Heuschmid, M. i Mertens, T. (1996). The UL97 product of human cytomegalovirus is an early-late protein with a nuclear localization but is not a nucleoside kinase. Journal of Virology, 70. (9), 6340-6346. Preuzeto s https://urn.nsk.hr/urn:nbn:hr:184:830706

Michel, Detlef, et al. "The UL97 product of human cytomegalovirus is an early-late protein with a nuclear localization but is not a nucleoside kinase." Journal of Virology, vol. 70, br. 9, 1996, str. 6340-6346. https://urn.nsk.hr/urn:nbn:hr:184:830706

Michel, Detlef, Ivica Pavić, Albert Zimmermann, Edith Haupt, Kirsten Wunderlich, Maria Heuschmid i Thomas Mertens. "The UL97 product of human cytomegalovirus is an early-late protein with a nuclear localization but is not a nucleoside kinase." Journal of Virology 70, br. 9 (1996): 6340-6346. https://urn.nsk.hr/urn:nbn:hr:184:830706

Michel, D., et al. (1996) 'The UL97 product of human cytomegalovirus is an early-late protein with a nuclear localization but is not a nucleoside kinase', Journal of Virology, 70(9), str. 6340-6346. Preuzeto s: https://urn.nsk.hr/urn:nbn:hr:184:830706 (Datum pristupa: 15.11.2024.)

Michel D, Pavić I, Zimmermann A, Haupt E, Wunderlich K, Heuschmid M, i sur.. The UL97 product of human cytomegalovirus is an early-late protein with a nuclear localization but is not a nucleoside kinase. Journal of Virology [Internet]. 1996. [pristupljeno 15.11.2024.];70(9):6340-6346. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:830706

D. Michel, et al., "The UL97 product of human cytomegalovirus is an early-late protein with a nuclear localization but is not a nucleoside kinase", Journal of Virology, vol. 70, br. 9, str. 6340-6346, 1996. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:830706. [Citirano: 15.11.2024.]

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Naslov (engleski)The UL97 product of human cytomegalovirus is an early-late protein with a nuclear localization but is not a nucleoside kinase
AutorDetlef Michel
AutorIvica Pavić
AutorAlbert Zimmermann
AutorEdith Haupt
AutorKirsten Wunderlich
AutorMaria Heuschmid
AutorThomas Mertens
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za zarazne bolesti)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Sažetak (engleski)
The temporal expression of the UL97 gene product during human cytomegalovirus (HCMV) infection of human foreskin fibroblasts (HFF) and subcellular localization of this protein were analyzed by using a polyclonal antiserum raised against a truncated UL97 protein of 47 kDa. The UL97 protein was detectable 16 h after infection by Western blot (immunoblot) analysis. Since only reduced UL97 expression occurred in the presence of two inhibitors of DNA replication, phosphonoacetic acid and ganciclovir, we conclude that UL97 is an early-late gene, requiring DNA replication for maximum expression. By indirect immunofluorescence, the protein could be visualized in the nuclei of virus-infected HFF 22 h after infection. Nuclear localization of the UL97 protein was also detected in thymidine kinase-deficient 143B cells infected with a recombinant vaccinia virus containing the entire UL97 open reading frame (ORF), as well as in HFF transiently expressing the entire UL97 ORF under the control of HCMV major immediate-early promoter. However, transiently expressed 5'-terminal deletion mutants of the UL97 ORF in addition showed a cytoplasmic localization of the UL97 protein, confirming the presence of a nuclear localization site in the N-terminal region of the protein. Our high-pressure liquid chromatography analyses confirmed the ganciclovir phosphorylation by the UL97 protein, but no specific phosphorylation of natural nucleosides was observed, indicating that the UL97 protein is not a nucleoside kinase. During plaque purification of recombinant UL97-deficient HCMV, this virus was growth defective ; hence, we presume that UL97 may be essential for the viral life cycle.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaJournal of Virology
Brojčani podacivol. 70, br. 9, str. 6340-6346
p-ISSN0022-538X
URN:NBNurn:nbn:hr:184:830706
Datum objave publikacije1996
URL dokumentahttp://bib.irb.hr/811812
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Zaštićeno autorskim pravom.
Datum i vrijeme pohrane2022-04-20 14:11:36