Antioxidants reduce or eliminate the negative effects of free radicals. Vitamin E is fat soluble vitamin with the best antioxidant properties. In nature, there are eight different types of this vitamin, such as α-, β-, γ-, δ-tocopherols and tocotrienols. Many antioxidants, including different types of vitamin E react with radicals at various speeds, hence their different antioxidant activity. This research analyses and determines the antioxidant activity of the individual isomers of vitamin E (α-, γ- and δ-tocopherol) and their combinations. The activity of the antioxidant sample was shown by losing the signal of DPPH radical and in the relation to the concentration calculated from Trolox method. Losses of the signal of α-, γ- and δ-tocopherols are much smaller compared to the combinations of the same isomers, based on which it can an be concluded that the antioxidant activity or the capacity to capture DPPH radicals is much higher in combinations of two or more isomers than in individual isomers. The antioxidant activity obtained by the DPPH method is different with regard to antioxidant activity obtained by the Trolox method which recognizes the masses of isomers in the sample. In determining the antioxidant activity using the Trolox method, the mass of vitamin E isomers in the sample taken into account, and it is proven that α-tocopherol has the highest antioxidant activity, while it is the lowest in the case of δ-tocopherol. In the comparison of the kinetic data and the statistical indicators for monophasic and biphasic models proves the best acceptability of the biphasic first-order kinetic model to describe the kinetics of DPPH radical disappearance in samples of vitamin E isomers and their mutual combination. The results showed that the reaction takes place in the two phases; first rapid phase during the initial 5 – 10 minutes of the reaction when follow the second much slower phase.