prikaz prve stranice dokumenta Immunohistochemical analysis of vocal cord polyps applying markers of squamous cell carcinogenesis
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Immunohistochemical analysis of vocal cord polyps applying markers of squamous cell carcinogenesis
Pathology - Research and Practice, 215 (2019), 1; 144-150. https://doi.org/10.1016/j.prp.2018.11.001
Braut, Tamara; Krstulja, Mira; Marijić, Blažen; Maržić, Diana; Kujundžić, Milodar; Brumini, Gordana; Vučinić, Damir; Oštarijaš, Eduard

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Braut, T., Krstulja, M., Marijić, B., Maržić, D., Kujundžić, M., Brumini, G. ... Oštarijaš, E. (2019). Immunohistochemical analysis of vocal cord polyps applying markers of squamous cell carcinogenesis. Pathology - Research and Practice, 215. (1), 144-150. doi: 10.1016/j.prp.2018.11.001

Braut, Tamara, et al. "Immunohistochemical analysis of vocal cord polyps applying markers of squamous cell carcinogenesis." Pathology - Research and Practice, vol. 215, br. 1, 2019, str. 144-150. https://doi.org/10.1016/j.prp.2018.11.001

Braut, Tamara, Mira Krstulja, Blažen Marijić, Diana Maržić, Milodar Kujundžić, Gordana Brumini, Damir Vučinić i Eduard Oštarijaš. "Immunohistochemical analysis of vocal cord polyps applying markers of squamous cell carcinogenesis." Pathology - Research and Practice 215, br. 1 (2019): 144-150. https://doi.org/10.1016/j.prp.2018.11.001

Braut, T., et al. (2019) 'Immunohistochemical analysis of vocal cord polyps applying markers of squamous cell carcinogenesis', Pathology - Research and Practice, 215(1), str. 144-150. doi: 10.1016/j.prp.2018.11.001

Braut T, Krstulja M, Marijić B, Maržić D, Kujundžić M, Brumini G, i sur.. Immunohistochemical analysis of vocal cord polyps applying markers of squamous cell carcinogenesis. Pathology - Research and Practice [Internet]. 2019. [pristupljeno 14.04.2025.];215(1):144-150. doi: 10.1016/j.prp.2018.11.001

T. Braut, et al., "Immunohistochemical analysis of vocal cord polyps applying markers of squamous cell carcinogenesis", Pathology - Research and Practice, vol. 215, br. 1, str. 144-150, 2019. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:342575. [Citirano: 14.04.2025.]

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Naslov (engleski)Immunohistochemical analysis of vocal cord polyps applying markers of squamous cell carcinogenesis
AutorTamara Braut
AutorMira Krstulja
AutorBlažen Marijić
AutorDiana Maržić
AutorMilodar Kujundžić
AutorGordana Brumini
AutorDamir Vučinić https://orcid.org/0000-0002-5804-0799
AutorEduard Oštarijaš
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za medicinsku informatiku)
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za opću patologiju i patološku anatomiju)
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za otorinolaringologiju)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Kliničke medicinske znanosti
Sažetak (engleski)
Objective The aim of the study was to perform a pathohistological and immunohistochemical analysis of squamous cell (SC) carcinogenesis markers on epithelial linings of vocal cord polyps. The vocal box, being a heavily burdened organ with intensive cell renewal and regenerative processes, is therefore a favourable environment for constant epithelial growth and hyperplasia. In our ongoing projects on laryngeal carcinogenesis and research on laryngeal tissue, we encountered atypia on diagnosed... Više nodules and polyps that are usually considered as benign formations, resulting from the above-mentioned cell renewal and regeneration, which lead to further investigation. The purpose was to see if changes in molecular markers of SC carcinogenesis follow, or, may appear in immunohistochemical (IHC) analysis, before histological atypia in standard haematoxylin- eosin (HE) staining, and contribute in early diagnosis of potentially suspect polyps. Methods After classical pathohistological (PH) analysis on HE slides, IHC analysis of EGFR, cyclin D1, p53, Ki-67, and IMP3 was performed on tissue microarrays of laryngeal tissue (50 samples), ranging from normal to hyperplastic lesions with no atypia (34 samples), low-grade atypia (11 samples), and high-grade atypia (5 samples). Results This study established an increase and correlation of EGFR, cyclin D1, p53, Ki-67 and IMP3 IHC expressions with pathohistological findings of dysplasia in glottic polypoid lesions. Low and high-grade dysplasia had statistically higher percentages of EGFR- positive cells than normal epithelium and simple hyperplasia (SH) (low vs. normal/SH P = 0.007 ; high vs. normal/SH P = 0.001). High-grade dysplasia had statistically more positive cells than low-grade dysplasia (P = 0.004), and low- grade dysplasia had statistically more positive cells than specimens without atypia (P = 0.007). The percentage of positive cells was statistically higher for cyclin D1, p53 and Ki- 67 in high- grade dysplasia versus low-grade dysplasia (cyclin D1 P = 0.011, p53 P = 0.002 ; Ki-67 P = 0.026 ; respectively) and versus normal epithelium and SH (cyclin D1 P = 0.003 ; p53 P = 0.001 ; Ki-67 P = 0.002 ; respectively). An increase of IMP3- positive cells with an increase of atypical changes in the laryngeal epithelium, from superficial towards basal layers was noticed, contrary to the usually seen positivity pattern of SC carcinogenesis markers from basal to superficial layers. A statistically significant difference of IMP3 IHC staining between the pathohistological groups (P = 0.003) was recorded. Conclusion Only polyps that present with simple hyperplasia as the greatest mucosal change can be considered as benign formations. Pathohistologically detected atypia in polypoid changes of vocal cords, confirmed by molecular atypia with an increase of SC carcinogenesis markers, suggest their inclusion in studies of laryngeal carcinogenesis. Our results suggest that in problematic cases IHC analysis could be of interest in detection of biological aggressiveness in polypoid laryngeal tissue and beneficiary for polyp patients’ follow-up. Further research of laryngeal carcinogenesis markers and their meaning in fibrovascular polyps is of interest. Sakrij dio sažetka
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaPathology - Research and Practice
Brojčani podacivol. 215, br. 1, str. 144-150
p-ISSN0344-0338
DOIhttps://doi.org/10.1016/j.prp.2018.11.001
URN:NBNurn:nbn:hr:184:342575
Datum objave publikacije2019
URL dokumentahttp://bib.irb.hr/969953
Vrsta resursaTekst
Prava pristupaZatvoreni pristup
Uvjeti korištenjaIkona licence Zaštićeno autorskim pravom.
Datum i vrijeme pohrane2022-02-21 13:50:47
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