prikaz prve stranice dokumenta Immunometabolic phenotype of BV-2 microglia cells upon murine cytomegalovirus infection
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Immunometabolic phenotype of BV-2 microglia cells upon murine cytomegalovirus infection
Journal of neurovirology, 25 (2019), 4; 496-507. https://doi.org/10.1007/s13365-019-00750-1
Kučić, Natalia; Rački, Valentino; Jurdana, Kristina; Marcelić, Marina; Grabušić, Kristina

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Kučić, N., Rački, V., Jurdana, K., Marcelić, M. i Grabušić, K. (2019). Immunometabolic phenotype of BV-2 microglia cells upon murine cytomegalovirus infection. Journal of neurovirology, 25. (4), 496-507. doi: 10.1007/s13365-019-00750-1

Kučić, Natalia, et al. "Immunometabolic phenotype of BV-2 microglia cells upon murine cytomegalovirus infection." Journal of neurovirology, vol. 25, br. 4, 2019, str. 496-507. https://doi.org/10.1007/s13365-019-00750-1

Kučić, Natalia, Valentino Rački, Kristina Jurdana, Marina Marcelić i Kristina Grabušić. "Immunometabolic phenotype of BV-2 microglia cells upon murine cytomegalovirus infection." Journal of neurovirology 25, br. 4 (2019): 496-507. https://doi.org/10.1007/s13365-019-00750-1

Kučić, N., et al. (2019) 'Immunometabolic phenotype of BV-2 microglia cells upon murine cytomegalovirus infection', Journal of neurovirology, 25(4), str. 496-507. doi: 10.1007/s13365-019-00750-1

Kučić N, Rački V, Jurdana K, Marcelić M, Grabušić K. Immunometabolic phenotype of BV-2 microglia cells upon murine cytomegalovirus infection. Journal of neurovirology [Internet]. 2019. [pristupljeno 15.04.2024.];25(4):496-507. doi: 10.1007/s13365-019-00750-1

N. Kučić, V. Rački, K. Jurdana, M. Marcelić i K. Grabušić, "Immunometabolic phenotype of BV-2 microglia cells upon murine cytomegalovirus infection", Journal of neurovirology, vol. 25, br. 4, str. 496-507, 2019. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:610414. [Citirano: 15.04.2024.]

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Podaci o radu
Naslov (engleski)Immunometabolic phenotype of BV-2 microglia cells upon murine cytomegalovirus infection
AutorNatalia Kučić
MBZ: 239812
AutorValentino Rački
AutorKristina Jurdana
AutorMarina Marcelić
AutorKristina Grabušić
MBZ: 270241
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za fiziologiju, imunologiju i patofiziologiju)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Sažetak (engleski)
Microglia are resident brain macrophages with key roles in development and brain homeostasis. Cytomegalovirus (CMV) readily infects microglia cells, even as a possible primary target of infection in development. Effects of CMV infection on a cellular level in microglia are still unclear ; therefore, the aim of this research was to assess the immunometabolic changes of BV-2 microglia cells following the murine cytomegalovirus (MCMV) infection. In light of that aim, we established an in vitro model of ramified BV-2 microglia (BV-2∅FCS, inducible nitric oxide synthase (iNOSlow), arginase-1 (Arg-1high), mannose receptor CD206high, and hypoxia-inducible factor 1α (HIF-1αlow)) to better replicate the in vivo conditions by removing FCS from the cultivation media, while the cells cultivated in 10% FCS DMEM displayed an ameboid morphology (BV-2FCS high, iNOShigh, Arg-1low, CD206low, and HIF- 1αhigh). Experiments were performed using both ramified and ameboid microglia, and both of them were permissive to productive viral infection. Our results indicate that MCMV significantly alters the immunometabolic phenotypic properties of BV-2 microglia cells through the manipulation of iNOS and Arg-1 expression patterns, along with an induction of a glycolytic shift in the infected cell cultures.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaJournal of neurovirology
Brojčani podacivol. 25, br. 4, str. 496-507
p-ISSN1355-0284
DOIhttps://doi.org/10.1007/s13365-019-00750-1
URN:NBNurn:nbn:hr:184:610414
Datum objave publikacije2019
ProjektŠifra: 13.06.1.3.45
Naziv (hrvatski): Endocitoza MHC molekula I razreda u mikroglijalnim stanicama inficiranih citomegalovirusom
Voditelj: Natalia Kučić
Pravna nadležnost: Hrvatska
Financijer: NadSve
Linija financiranja: Sveučilište u Rijeci
URL dokumentahttp://bib.irb.hr/1016320
Vrsta resursaTekst
Prava pristupaOgraničen pristup
Uvjeti korištenjaIkona licence Zaštićeno autorskim pravom.
Datum i vrijeme pohrane2022-02-21 13:39:52