prikaz prve stranice dokumenta Francisella tularensis D-Ala D-Ala Carboxypeptidase DacD Is Involved in Intracellular Replication and It Is Necessary for Bacterial Cell Wall Integrity
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Francisella tularensis D-Ala D-Ala Carboxypeptidase DacD Is Involved in Intracellular Replication and It Is Necessary for Bacterial Cell Wall Integrity
Frontiers in Cellular and Infection Microbiology, 8 (2018); 111. https://doi.org/10.3389/fcimb.2018.00111
Spidlova, Petra; Stojkova, Pavla; Dankova, Vera; Senitkova, Iva; Šantić, Marina; Pinkas, Dominik; Philimonenko, Vlada; Stulik, Jiri

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Spidlova, P., Stojkova, P., Dankova, V., Senitkova, I., Šantić, M., Pinkas, D. ... Stulik, J. (2018). Francisella tularensis D-Ala D-Ala Carboxypeptidase DacD Is Involved in Intracellular Replication and It Is Necessary for Bacterial Cell Wall Integrity. Frontiers in Cellular and Infection Microbiology, 8.. doi: 10.3389/fcimb.2018.00111

Spidlova, Petra, et al. "Francisella tularensis D-Ala D-Ala Carboxypeptidase DacD Is Involved in Intracellular Replication and It Is Necessary for Bacterial Cell Wall Integrity." Frontiers in Cellular and Infection Microbiology, vol. 8, 2018. https://doi.org/10.3389/fcimb.2018.00111

Spidlova, Petra, Pavla Stojkova, Vera Dankova, Iva Senitkova, Marina Šantić, Dominik Pinkas, Vlada Philimonenko i Jiri Stulik. "Francisella tularensis D-Ala D-Ala Carboxypeptidase DacD Is Involved in Intracellular Replication and It Is Necessary for Bacterial Cell Wall Integrity." Frontiers in Cellular and Infection Microbiology 8 (2018). https://doi.org/10.3389/fcimb.2018.00111

Spidlova, P., et al. (2018) 'Francisella tularensis D-Ala D-Ala Carboxypeptidase DacD Is Involved in Intracellular Replication and It Is Necessary for Bacterial Cell Wall Integrity', Frontiers in Cellular and Infection Microbiology, 8. doi: 10.3389/fcimb.2018.00111

Spidlova P, Stojkova P, Dankova V, Senitkova I, Šantić M, Pinkas D, i sur.. Francisella tularensis D-Ala D-Ala Carboxypeptidase DacD Is Involved in Intracellular Replication and It Is Necessary for Bacterial Cell Wall Integrity. Frontiers in Cellular and Infection Microbiology [Internet]. 10.04.2018. [pristupljeno 25.12.2024.];8. doi: 10.3389/fcimb.2018.00111

P. Spidlova, et al., "Francisella tularensis D-Ala D-Ala Carboxypeptidase DacD Is Involved in Intracellular Replication and It Is Necessary for Bacterial Cell Wall Integrity", Frontiers in Cellular and Infection Microbiology, vol. 8, Travanj 2018. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:851493. [Citirano: 25.12.2024.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:184:851493
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Naslov (engleski)Francisella tularensis D-Ala D-Ala Carboxypeptidase DacD Is Involved in Intracellular Replication and It Is Necessary for Bacterial Cell Wall Integrity
AutorPetra Spidlova
AutorPavla Stojkova
AutorVera Dankova
AutorIva Senitkova
AutorMarina Šantić
AutorDominik Pinkas
AutorVlada Philimonenko
AutorJiri Stulik
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za mikrobiologiju i parazitologiju)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Kliničke medicinske znanosti
Medicinska mikrobiologija
Sažetak (engleski)
D-alanyl-D-alanine carboxypeptidase, product of dacD gene in Francisella, belongs to penicillin binding proteins (PBPs) and is involved in remodeling of newly synthetized peptidoglycan. In E. coli, PBPs are synthetized in various growth phases and they are able to substitute each other to a certain extent. The DacD protein was found to be accumulated in fraction enriched in membrane proteins from severely attenuated dsbA deletion mutant strain. It has been presumed that the DsbA is not a virulence factor by itself but that its substrates, whose correct folding and topology are dependent on the DsbA oxidoreductase and/or isomerase activities, are the primary virulence factors. Here we demonstrate that Francisella DacD is required for intracellular replication and virulence in mice. The dacD insertion mutant strain showed higher sensitivity to acidic pH, high temperature and high osmolarity when compared to the wild-type. Eventually, transmission electron microscopy revealed differences in mutant bacteria in both the size and defects in outer membrane underlying its SDS and serum sensitivity. Taken together these results suggest DacD plays an important role in Francisella pathogenicity.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaFrontiers in Cellular and Infection Microbiology
Brojčani podacivol. 8, 111
e-ISSN2235-2988
DOIhttps://doi.org/10.3389/fcimb.2018.00111
URN:NBNurn:nbn:hr:184:851493
Datum objave publikacije2018-04-10
ProjektŠifra: IP-2016-06-9003
Naziv (hrvatski): Uloga unutarstaničnog života Francisella tularensis u patogenezi eksperimentalne tularemije
Kratica: FRANCYCLE
Voditelj: Marina Šantić
Pravna nadležnost: Hrvatska
Financijer: HRZZ
Linija financiranja: IP
URL dokumentahttp://journal.frontiersin.org/article/10.3389/fcimb.2018.00111/full
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 4.0 međunarodna (CC BY 4.0)
Datum i vrijeme pohrane2022-01-28 09:11:05