Nowadays, people are getting more and more obsessed with the “Hollywood” smile that all forms of media serve in one way or another (movies, TV shows/series, social networks, etc.). Therefore, an increasing number of people are starting to wear orthodontic appliances, unaware of the potential harmful effects that its components could have on the human organism. The release of metal ions from orthodontic materials is becoming a growing concern of the orthodontic world because, in addition to causing contact dermatitis, acute or chronic poisonings are also possible to occur. In addition, metal ions can cause oxidative stress, by which their genotoxic and cytotoxic effects could be manifested. The aim of this study was to examine the effects of released ions (Ni2+, Mo4+ and Cr3+) on the vitality and viability of two yeast strains: Saccharomyces cerevisiae W303 and wild type. The research was done in two parts – on YPD growth media and in artificial saliva. Growth media and artificial saliva samples with the amount of ions released from orthodontic braces and brackets over 3, 7 and 14 days were used in each method. Viability was observed by the change in the number of the cells of both yeast strains over 24 hour-growth in treated growth media and artificial saliva samples. The results were expressed via growth curves and counted cells using a LUNA-FLTM Dual fluorescence cell counter. On the other hand, vitality was observed by the change in cell's metabolic activity using two method. First – cell staining with the LIVE/DEAD Yeast Viability Kit, afterwhich the cells were microscoped using a fluorescence microscopy, and the second – XTT test. Also, fluorimetric measures of intracellular oxidation determined the amount of ROS generated in yeast cells as a result of active metabolism and growth on depleted media. The ions of orthodontic braces and rings affect the vitality and viability of both strains of the yeast Saccharomyces cerevisiae, especially the W303 strain in all parameters.