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Cytomegalovirus Generates Assembly Compartment in the Early Phase of Infection by Perturbation of Host-Cell Factors Recruitment at the Early Endosome/Endosomal Recycling Compartment/Trans-Golgi Interface
Frontiers in Cell and Developmental Biology, 8 (2020); 1-24. https://doi.org/10.3389/fcell.2020.563607
Lučin, Pero; Jug Vučko, Natalia; Karleuša, Ljerka; Mahmutefendić Lučin, Hana; Blagojević Zagorac, Gordana; Lisnić, Berislav; Pavišić, Valentino; Marcelić, Marina; Grabušić, Kristina; Brizić, Ilija; Lukanović Jurić, Silvija

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Lučin, P., Jug Vučko, N., Karleuša, Lj., Mahmutefendić Lučin, H., Blagojević Zagorac, G., Lisnić, B. ... Lukanović Jurić, S. (2020). Cytomegalovirus Generates Assembly Compartment in the Early Phase of Infection by Perturbation of Host-Cell Factors Recruitment at the Early Endosome/Endosomal Recycling Compartment/Trans-Golgi Interface. Frontiers in Cell and Developmental Biology, 8., 1-24. doi: 10.3389/fcell.2020.563607

Lučin, Pero, et al. "Cytomegalovirus Generates Assembly Compartment in the Early Phase of Infection by Perturbation of Host-Cell Factors Recruitment at the Early Endosome/Endosomal Recycling Compartment/Trans-Golgi Interface." Frontiers in Cell and Developmental Biology, vol. 8, 2020, str. 1-24. https://doi.org/10.3389/fcell.2020.563607

Lučin, Pero, Natalia Jug Vučko, Ljerka Karleuša, Hana Mahmutefendić Lučin, Gordana Blagojević Zagorac, Berislav Lisnić, Valentino Pavišić, et al. "Cytomegalovirus Generates Assembly Compartment in the Early Phase of Infection by Perturbation of Host-Cell Factors Recruitment at the Early Endosome/Endosomal Recycling Compartment/Trans-Golgi Interface." Frontiers in Cell and Developmental Biology 8 (2020): 1-24. https://doi.org/10.3389/fcell.2020.563607

Lučin, P., et al. (2020) 'Cytomegalovirus Generates Assembly Compartment in the Early Phase of Infection by Perturbation of Host-Cell Factors Recruitment at the Early Endosome/Endosomal Recycling Compartment/Trans-Golgi Interface', Frontiers in Cell and Developmental Biology, 8, str. 1-24. doi: 10.3389/fcell.2020.563607

Lučin P, Jug Vučko N, Karleuša Lj, Mahmutefendić Lučin H, Blagojević Zagorac G, Lisnić B, i sur.. Cytomegalovirus Generates Assembly Compartment in the Early Phase of Infection by Perturbation of Host-Cell Factors Recruitment at the Early Endosome/Endosomal Recycling Compartment/Trans-Golgi Interface. Frontiers in Cell and Developmental Biology [Internet]. 2020. [pristupljeno 24.12.2024.];8:1-24. doi: 10.3389/fcell.2020.563607

P. Lučin, et al., "Cytomegalovirus Generates Assembly Compartment in the Early Phase of Infection by Perturbation of Host-Cell Factors Recruitment at the Early Endosome/Endosomal Recycling Compartment/Trans-Golgi Interface", Frontiers in Cell and Developmental Biology, vol. 8, str. 1-24, 2020. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:376839. [Citirano: 24.12.2024.]

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Naslov (engleski)Cytomegalovirus Generates Assembly Compartment in the Early Phase of Infection by Perturbation of Host-Cell Factors Recruitment at the Early Endosome/Endosomal Recycling Compartment/Trans-Golgi Interface
AutorPero Lučin
AutorNatalia Jug Vučko
AutorLjerka Karleuša
AutorHana Mahmutefendić Lučin
AutorGordana Blagojević Zagorac
AutorBerislav Lisnić
AutorValentino Pavišić
AutorMarina Marcelić
AutorKristina Grabušić
AutorIlija Brizić
AutorSilvija Lukanović Jurić
Autorova ustanovaSveučilište Sjever
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za fiziologiju, imunologiju i patofiziologiju)
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za histologiju i embriologiju)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Sažetak (engleski)
Beta-herpesviruses develop a unique structure within the infected cell known as an assembly compartment (AC). This structure, as large as the nucleus, is composed of host-cell-derived membranous elements. The biogenesis of the AC and its contribution to the final stages of beta-herpesvirus assembly are still unclear. In this study, we performed a spatial and temporal analysis of the AC in cells infected with murine CMV (MCMV), a member of the beta-herpesvirus family, using a panel of markers that characterize membranous organelle system. Out of 64 markers that were analyzed, 52 were cytosolic proteins that are recruited to membranes as components of membrane-shaping regulatory cascades. The analysis demonstrates that MCMV infection extensively reorganizes interface between early endosomes (EE), endosomal recycling compartment (ERC), and the trans-Golgi network (TGN), resulting in expansion of various EE-ERC-TGN intermediates that fill the broad area of the inner AC. These intermediates are displayed as over-recruitment of host-cell factors that control membrane flow at the EE-ERC-TGN interface. Most of the reorganization is accomplished in the early (E) phase of infection, indicating that the AC biogenesis is controlled by MCMV early genes. Although it is known that CMV infection affects the expression of a large number of host-cell factors that control membranous system, analysis of the host-cell transcriptome and protein expression in the E phase of infection demonstrated no sufficiently significant alteration in expression levels of analyzed markers. Thus, our study demonstrates that MCMV-encoded early phase function targets recruitment cascades of host cell-factors that control membranous flow at the EE-ERC-TGN interface in order to initiate the development of the AC.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaFrontiers in Cell and Developmental Biology
Brojčani podacivol. 8, str. 1-24, 563607
e-ISSN2296-634X
DOIhttps://doi.org/10.3389/fcell.2020.563607
URN:NBNurn:nbn:hr:184:376839
Datum objave publikacije2020
ProjektŠifra: IP-2014-09-9564
Naziv (hrvatski): Karakterizacija preuređivanja endosomalnih membrana tijekom rane faze infekcije mišjim citomegalovirusom koje vodi k formiranju kompleksa za stvaranje viriona
Naziv (engleski): Characterization of endosomal remodelling during the early phase of murine cytomegalovirus infection that leads to the development of the assembly complex
Kratica: AsCo
Voditelj: Pero Lučin
Pravna nadležnost: Hrvatska
Financijer: HRZZ
Linija financiranja: IP
ProjektŠifra: uniri-biomed-18-88
Naziv (hrvatski): Citomegalovirusni odjeljak za sklapanje viriona
Naziv (engleski): Cytomegalovirus assambly compartment
Voditelj: Pero Lučin
Pravna nadležnost: Hrvatska
Financijer: NadSve
Linija financiranja: Nema
URL dokumentahttps://www.frontiersin.org/article/10.3389/fcell.2020.563607/full
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 4.0 međunarodna (CC BY 4.0)
Datum i vrijeme pohrane2021-01-21 21:56:27