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Chromatographic Monoliths for High-Throughput Immunoaffinity Isolation of Transferrin from Human Plasma
Croatica Chemica Acta, 89 (2016), 2; 203-211. https://doi.org/10.5562/cca2815
Trbojević-Akmačić, Irena; Nemec, Blaž; Vidic, Urška; Malić, Suzana; Miklić, Karmela; Černigoj, Urh; Vidič, Jana; Lendero Krajnc, Nika; Štrancar, Aleš; Lauc, Gordan; Lenac Roviš, Tihana; Pučić-Baković, Maja

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Trbojević Akmačić, I., Nemec, B., Vidic, U., Malić, S., Miklić, K., Černigoj, U. ... Pučić Baković, M. (2016). Chromatographic Monoliths for High-Throughput Immunoaffinity Isolation of Transferrin from Human Plasma. Croatica Chemica Acta, 89. (2), 203-211. doi: 10.5562/cca2815

Trbojević Akmačić, Irena, et al. "Chromatographic Monoliths for High-Throughput Immunoaffinity Isolation of Transferrin from Human Plasma." Croatica Chemica Acta, vol. 89, br. 2, 2016, str. 203-211. https://doi.org/10.5562/cca2815

Trbojević Akmačić, Irena, Blaž Nemec, Urška Vidic, Suzana Malić, Karmela Miklić, Urh Černigoj, Jana Vidič, et al. "Chromatographic Monoliths for High-Throughput Immunoaffinity Isolation of Transferrin from Human Plasma." Croatica Chemica Acta 89, br. 2 (2016): 203-211. https://doi.org/10.5562/cca2815

Trbojević Akmačić, I., et al. (2016) 'Chromatographic Monoliths for High-Throughput Immunoaffinity Isolation of Transferrin from Human Plasma', Croatica Chemica Acta, 89(2), str. 203-211. doi: 10.5562/cca2815

Trbojević Akmačić I, Nemec B, Vidic U, Malić S, Miklić K, Černigoj U, i sur.. Chromatographic Monoliths for High-Throughput Immunoaffinity Isolation of Transferrin from Human Plasma. Croatica Chemica Acta [Internet]. 2016. [pristupljeno 04.12.2024.];89(2):203-211. doi: 10.5562/cca2815

I. Trbojević Akmačić, et al., "Chromatographic Monoliths for High-Throughput Immunoaffinity Isolation of Transferrin from Human Plasma", Croatica Chemica Acta, vol. 89, br. 2, str. 203-211, 2016. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:695580. [Citirano: 04.12.2024.]

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Naslov (engleski)Chromatographic Monoliths for High-Throughput Immunoaffinity Isolation of Transferrin from Human Plasma
AutorIrena Trbojević-Akmačić
MBZ: 351184
AutorBlaž Nemec
AutorUrška Vidic
AutorSuzana Malić
AutorKarmela Miklić
AutorUrh Černigoj
AutorJana Vidič
AutorNika Lendero Krajnc
AutorAleš Štrancar
AutorGordan Lauc
MBZ: 201852
AutorTihana Lenac Roviš
AutorMaja Pučić-Baković
MBZ: 310572
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Centar za proteomiku)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Sažetak (engleski)
Changes in protein glycosylation are related to different diseases and have a potential as diagnostic and prognostic disease biomarkers. Transferrin (Tf) glycosylation changes are common marker for congenital disorders of glycosylation. However, biological interindividual variability of Tf N-glycosylation and genes involved in glycosylation regulation are not known. Therefore, high-throughput Tf isolation method and large scale glycosylation studies are needed in order to address these questions. Due to their unique chromatographic properties, the use of chromatographic monoliths enables very fast analysis cycle, thus significantly increasing sample preparation throughput. Here, we are describing characterization of novel immunoaffinity-based monolithic columns in a 96-well plate format for specific high-throughput purification of human Tf from blood plasma. We optimized the isolation and glycan preparation procedure for subsequent ultra performance liquid chromatography (UPLC) analysis of Tf N-glycosylation and managed to increase the sensitivity for approximately three times compared to initial experimental conditions, with very good reproducibility.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaCroatica Chemica Acta
Brojčani podacivol. 89, br. 2, str. 203-211
p-ISSN0011-1643
DOIhttps://doi.org/10.5562/cca2815
URN:NBNurn:nbn:hr:184:695580
Datum objave publikacije2016
ProjektŠifra: 278535
Naziv (hrvatski): Methods for high-throughput (HTP) analysis of protein glycosylation
Kratica: HIGHGLYCAN
Pravna nadležnost: eu
Financijer: EC
Linija financiranja: FP7
ProjektŠifra: 324400
Naziv (hrvatski): Methods for high-throughput glycoproteomic analysis
Kratica: HTP-GLYCOMET
Pravna nadležnost: eu
Financijer: EC
Linija financiranja: FP7
URL dokumentahttp://bib.irb.hr/1002539
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 4.0 međunarodna (CC BY 4.0)
Datum i vrijeme pohrane2020-04-19 14:18:45