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CMV Late Phase-Induced mTOR Activation Is Essential for Efficient Virus Replication in Polarized Human Macrophages : Antiviral Effects of mTOR Inhibitors
American Journal of Transplantation, 12 (2012), 6; 1458-1468. https://doi.org/10.1111/j.1600-6143.2012.04002.x
Poglitsch, M.; Weichhart, T.; Hecking, M.; Werzowa, J.; Katholnig, K.; Antlanger, M.; Krmpotić, Astrid; Jonjić, Stipan; Hörl, W. H.; Zlabinger, G. J.; Puchhammer, E.; Säemann, M. D.

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Poglitsch, M., Weichhart, T., Hecking, M., Werzowa, J., Katholnig, K., Antlanger, M. ... Säemann, M. D. (2012). CMV Late Phase-Induced mTOR Activation Is Essential for Efficient Virus Replication in Polarized Human Macrophages : Antiviral Effects of mTOR Inhibitors. American Journal of Transplantation, 12. (6), 1458-1468. doi: 10.1111/j.1600-6143.2012.04002.x

Poglitsch, M., et al. "CMV Late Phase-Induced mTOR Activation Is Essential for Efficient Virus Replication in Polarized Human Macrophages : Antiviral Effects of mTOR Inhibitors." American Journal of Transplantation, vol. 12, br. 6, 2012, str. 1458-1468. https://doi.org/10.1111/j.1600-6143.2012.04002.x

Poglitsch, M., T. Weichhart, M. Hecking, J. Werzowa, K. Katholnig, M. Antlanger, Astrid Krmpotić, et al. "CMV Late Phase-Induced mTOR Activation Is Essential for Efficient Virus Replication in Polarized Human Macrophages : Antiviral Effects of mTOR Inhibitors." American Journal of Transplantation 12, br. 6 (2012): 1458-1468. https://doi.org/10.1111/j.1600-6143.2012.04002.x

Poglitsch, M., et al. (2012) 'CMV Late Phase-Induced mTOR Activation Is Essential for Efficient Virus Replication in Polarized Human Macrophages : Antiviral Effects of mTOR Inhibitors', American Journal of Transplantation, 12(6), str. 1458-1468. doi: 10.1111/j.1600-6143.2012.04002.x

Poglitsch M, Weichhart T, Hecking M, Werzowa J, Katholnig K, Antlanger M, i sur.. CMV Late Phase-Induced mTOR Activation Is Essential for Efficient Virus Replication in Polarized Human Macrophages : Antiviral Effects of mTOR Inhibitors. American Journal of Transplantation [Internet]. 06.2012. [pristupljeno 25.12.2024.];12(6):1458-1468. doi: 10.1111/j.1600-6143.2012.04002.x

M. Poglitsch, et al., "CMV Late Phase-Induced mTOR Activation Is Essential for Efficient Virus Replication in Polarized Human Macrophages : Antiviral Effects of mTOR Inhibitors", American Journal of Transplantation, vol. 12, br. 6, str. 1458-1468, Lipanj 2012. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:984654. [Citirano: 25.12.2024.]

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Naslov (engleski)CMV Late Phase-Induced mTOR Activation Is Essential for Efficient Virus Replication in Polarized Human Macrophages : Antiviral Effects of mTOR Inhibitors
AutorM. Poglitsch
AutorT. Weichhart
AutorM. Hecking
AutorJ. Werzowa
AutorK. Katholnig
AutorM. Antlanger
AutorAstrid Krmpotić
AutorStipan Jonjić
AutorW. H. Hörl
AutorG. J. Zlabinger
AutorE. Puchhammer
AutorM. D. Säemann
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za histologiju i embriologiju)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Sažetak (engleski)
Human cytomegalovirus (CMV) remains one of the most important pathogens following solid-organ transplantation. Mounting evidence indicates that mammalian target of rapamycin (mTOR) inhibitors may decrease the incidence of CMV infection in solid- organ recipients. Here we aimed at elucidating the molecular mechanisms of this effect by employing a human CMV (HCMV) infection model in human macrophages, since myeloid cells are the principal in vivo targets of HCMV. We demonstrate a highly di- vergent host cell permissiveness for HCMV with opti- mal infection susceptibility in M2 but not M1 polarized macrophages. Employing an ultrahigh purified HCMV stock we observed rapamycin-independent viral entry and induction of IFN-b transcripts, but no proinflam- matory cytokines or mitogen-activated protein kinases and mTOR activation early after infection. However, in the late infection phase, sustained mTOR activa- tion was observed in HCMV-infected cells and was required for efficient viral protein synthesis including the viral late phase proteins pUL-44 and pp65. Accord- ingly, rapamycin strongly suppressed CMV replication 3 and 5 days postinfection in macrophages. In conclu- sion, these data indicate that mTOR is essential for virus replication during late phases of the viral cycle in myeloid cells and might explain the potent anti-CMV effects of mTOR inhibitors after organ transplantation
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaAmerican Journal of Transplantation
Brojčani podacivol. 12, br. 6, str. 1458-1468
p-ISSN1600-6135
DOIhttps://doi.org/10.1111/j.1600-6143.2012.04002.x
URN:NBNurn:nbn:hr:184:984654
Datum objave publikacije2012-06
Datum objave publikacije2012-03-05
URL dokumentahttp://doi.wiley.com/10.1111/j.1600-6143.2012.04002.x
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje-Nekomercijalno-Bez prerada 4.0 međunarodna (CC BY-NC-ND 4.0)
Datum i vrijeme pohrane2018-11-08 10:56:29