prikaz prve stranice dokumenta MIgGGly (mouse IgG glycosylation analysis) - a high-throughput method for studying Fc-linked IgG N-glycosylation in mice with nanoUPLC-ESI-MS
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MIgGGly (mouse IgG glycosylation analysis) - a high-throughput method for studying Fc-linked IgG N-glycosylation in mice with nanoUPLC-ESI-MS
Scientific Reports, 8 (2018); 13688. https://doi.org/10.1038/s41598-018-31844-1
Zaytseva, Olga O.; Jansen, Bas C.; Hinić, Maja; Mrčela, Mia; Genadij, Razdorov; Stojković, Ranko; Erhardt, Julija; Brizić, Ilija; Jonjić, Stipan; Pezer, Marija; Lauc, Gordan

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Zaytseva, O. O., Jansen, B. C., Hinić, M., Mrčela, M., Genadij, R., Stojković, R. ... Lauc, G. (2018). MIgGGly (mouse IgG glycosylation analysis) - a high-throughput method for studying Fc-linked IgG N-glycosylation in mice with nanoUPLC-ESI-MS. Scientific Reports, 8.. doi: 10.1038/s41598-018-31844-1

Zaytseva, Olga O., et al. "MIgGGly (mouse IgG glycosylation analysis) - a high-throughput method for studying Fc-linked IgG N-glycosylation in mice with nanoUPLC-ESI-MS." Scientific Reports, vol. 8, 2018. https://doi.org/10.1038/s41598-018-31844-1

Zaytseva, Olga O., Bas C. Jansen, Maja Hinić, Mia Mrčela, Razdorov Genadij, Ranko Stojković, Julija Erhardt, et al. "MIgGGly (mouse IgG glycosylation analysis) - a high-throughput method for studying Fc-linked IgG N-glycosylation in mice with nanoUPLC-ESI-MS." Scientific Reports 8 (2018). https://doi.org/10.1038/s41598-018-31844-1

Zaytseva, O. O., et al. (2018) 'MIgGGly (mouse IgG glycosylation analysis) - a high-throughput method for studying Fc-linked IgG N-glycosylation in mice with nanoUPLC-ESI-MS', Scientific Reports, 8. doi: 10.1038/s41598-018-31844-1

Zaytseva OO, Jansen BC, Hinić M, Mrčela M, Genadij R, Stojković R, i sur.. MIgGGly (mouse IgG glycosylation analysis) - a high-throughput method for studying Fc-linked IgG N-glycosylation in mice with nanoUPLC-ESI-MS. Scientific Reports [Internet]. 12.08.2018. [pristupljeno 26.12.2024.];8. doi: 10.1038/s41598-018-31844-1

O. O. Zaytseva, et al., "MIgGGly (mouse IgG glycosylation analysis) - a high-throughput method for studying Fc-linked IgG N-glycosylation in mice with nanoUPLC-ESI-MS", Scientific Reports, vol. 8, Kolovoz 2018. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:098355. [Citirano: 26.12.2024.]

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Naslov (engleski)MIgGGly (mouse IgG glycosylation analysis) - a high-throughput method for studying Fc-linked IgG N-glycosylation in mice with nanoUPLC-ESI-MS
AutorOlga O. Zaytseva
AutorBas C. Jansen
AutorMaja Hinić
AutorMia Mrčela
AutorRazdorov Genadij
AutorRanko Stojković
AutorJulija Erhardt
AutorIlija Brizić
AutorStipan Jonjić
AutorMarija Pezer
AutorGordan Lauc
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za histologiju i embriologiju)
Znanstveno / umjetničko
područje, polje i grana		PRIRODNE ZNANOSTI
Biologija
Sažetak (engleski)
Immunoglobulin G (IgG) N-glycosylation is crucial for its effector functions. It is a complex trait, and large sample sets are needed to discover multiple genetic factors that underlie it. While in humans such high-throughput studies of IgG N-glycans became usual, only one has been carried out in mice. Here we describe and validate a method for the relative quantification of IgG Fc-linked N-glycans in a subclass- specific manner using nano-reverse phase liquid chromatography coupled with mass-spectrometry (nanoRP-LC-MS) applied to murine IgG. High-throughput data processing is ensured by the LaCyTools software. We have shown that IgG isolation procedure is the main source of technical variation in the current protocol. The major glycoforms were quantified reliably with coefficients of variation below 6% for all the analytes with relative abundances above 5%. We have applied our method to a sample set of 3 inbred strains: BALB/c, C57BL/6 and C3H and observed differences in subclass-specific and strain - specific N - glycosylation of IgG, suggesting a significant genetic component in the regulation of Fc- linked IgG N - glycosylation
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaScientific Reports
Brojčani podacivol. 8, 13688
e-ISSN2045-2322
DOIhttps://doi.org/10.1038/s41598-018-31844-1
URN:NBNurn:nbn:hr:184:098355
Datum objave publikacije2018-08-12
URL dokumentahttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6135756/pdf/41598_2018_Article_31844.pdf
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje-Nekomercijalno-Bez prerada 4.0 međunarodna (CC BY-NC-ND 4.0)
Datum i vrijeme pohrane2018-09-29 11:38:22