Enterococcus faecalis bacterium has recently begun to pay attention due to the more frequent hospital infection and other infections. They are naturally found in the intestinal flora of man and animal. But when they reach a place where they are not part of a natural flora can cause inflammation. Most commonly, we are talking about urinary system, which as a result of growth and reproduction cause urine infestation. In this paper, we investigated virulence factors and sensitivity to the antibiotics of enterococci isolated from urine. Virulence factors who were investigated are protease enzymes, lipases, gelatinases, catalases; hemolysis, lysozyme resistance, mobility, extracellular mucus and biofilm, aggregation and sensitivity to antibiotics. After the study has been shown, all catalase strains are negative, while they are all esculin positive. In more than 80% of isolates, γ-hemolysis has been proven. Of the other enzymes protease is produced by 59% isolates, 95% lipase and 70% gelatinase. The tendency to create biofilm and extracellular mucus has 73% bacteria. At concentrations of 60 mg / ml lysozyme, 35% bacteria are susceptible, while all isolates with lysozyme lower concetration (20, 40 mg/ml lysozyme) are resistant. For aggregation we can say that 31% of bacteria aggregate in percentage more than 90%. Of the antibiotics it is important to distinguish gentamicin with resistance to 23% of isolates and norfloxacin with 20% isolates. E. faecalis possesses several virulence factors, among which the most important are extracellular mucus production and biofilms production, and their ability to degrade collagen in the body by the gelatin enzyme