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Quantitative analysis of endocytic recycling of membrane proteins by monoclonal antibody-based recycling assays
Journal of Cellular Physiology, 232 (2017), 3; 463-476. https://doi.org/10.1002/jcp.25503
Blagojević Zagorac, Gordana; Mahmutefendić, Hana; Mačešić, Senka; Karleuša, Ljerka; Lučin, Pero

Citirajte ovaj rad

Blagojević Zagorac, G., Mahmutefendić, H., Mačešić, S., Karleuša, Lj. i Lučin, P. (2017). Quantitative analysis of endocytic recycling of membrane proteins by monoclonal antibody-based recycling assays. Journal of Cellular Physiology, 232. (3), 463-476. doi: 10.1002/jcp.25503

Blagojević Zagorac, Gordana, et al. "Quantitative analysis of endocytic recycling of membrane proteins by monoclonal antibody-based recycling assays." Journal of Cellular Physiology, vol. 232, br. 3, 2017, str. 463-476. https://doi.org/10.1002/jcp.25503

Blagojević Zagorac, Gordana, Hana Mahmutefendić, Senka Mačešić, Ljerka Karleuša i Pero Lučin. "Quantitative analysis of endocytic recycling of membrane proteins by monoclonal antibody-based recycling assays." Journal of Cellular Physiology 232, br. 3 (2017): 463-476. https://doi.org/10.1002/jcp.25503

Blagojević Zagorac, G., et al. (2017) 'Quantitative analysis of endocytic recycling of membrane proteins by monoclonal antibody-based recycling assays', Journal of Cellular Physiology, 232(3), str. 463-476. doi: 10.1002/jcp.25503

Blagojević Zagorac G, Mahmutefendić H, Mačešić S, Karleuša Lj, Lučin P. Quantitative analysis of endocytic recycling of membrane proteins by monoclonal antibody-based recycling assays. Journal of Cellular Physiology [Internet]. 2017. [pristupljeno 03.12.2024.];232(3):463-476. doi: 10.1002/jcp.25503

G. Blagojević Zagorac, H. Mahmutefendić, S. Mačešić, Lj. Karleuša i P. Lučin, "Quantitative analysis of endocytic recycling of membrane proteins by monoclonal antibody-based recycling assays", Journal of Cellular Physiology, vol. 232, br. 3, str. 463-476, 2017. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:221896. [Citirano: 03.12.2024.]

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Naslov (engleski)Quantitative analysis of endocytic recycling of membrane proteins by monoclonal antibody-based recycling assays
AutorGordana Blagojević Zagorac
AutorHana Mahmutefendić
AutorSenka Mačešić
AutorLjerka Karleuša
AutorPero Lučin
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za fiziologiju, imunologiju i patofiziologiju)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Fiziologija čovjeka
Sažetak (engleski)
In this report, we present an analysis of several recycling protocols based on labeling of membrane proteins with specific monoclonal antibodies (mAbs). We analyzed recycling of membrane proteins that are internalized by clathrin-dependent endocytosis, represented by the transferrin receptor, and by clathrin-independent endocytosis, represented by the Major Histocompatibility Class I molecules. Cell surface membrane proteins were labeled with mAbs and recycling of mAb:protein complexes was determined by several approaches. Our study demonstrates that direct and indirect detection of recycled mAb:protein complexes at the cell surface underestimate the recycling pool, especially for clathrin-dependent membrane proteins that are rapidly reinternalized after recycling. Recycling protocols based on the capture of recycled mAb:protein complexes require the use of the Alexa Fluor 488 conjugated secondary antibodies or FITC-conjugated secondary antibodies in combination with inhibitors of endosomal acidification and degradation. Finally, protocols based on the capture of recycled proteins that are labeled with Alexa Fluor 488 conjugated primary antibodies and quenching of fluorescence by the anti-Alexa Fluor 488 displayed the same quantitative assessment of recycling as the antibody-capture protocols.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeZavršna verzija rukopisa prihvaćena za objavljivanje (postprint)
Naslov časopisaJournal of Cellular Physiology
Brojčani podacivol. 232, br. 3, str. 463-476
p-ISSN0021-9541
e-ISSN1097-4652
DOIhttps://doi.org/10.1002/jcp.25503
URN:NBNurn:nbn:hr:184:221896
Datum objave publikacije2017
ProjektŠifra: HRZZ-IP-2014-09-9564
Naziv (hrvatski): Karakterizacija preuređivanja endosomalnih membranatijekom rane faze infekcije mišjim citomegalovirusom koje vodi k formiranju kompleksa za stvaranje viriona (Pero Lučin)
Pravna nadležnost: Hrvatska
Financijer: MZOS
URL dokumentahttp://bib.irb.hr/882266
URL dokumentahttp://onlinelibrary.wiley.com/doi/10.1002/jcp.25503/abstract
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje-Nekomercijalno-Bez prerada 4.0 međunarodna (CC BY-NC-ND 4.0)
Datum i vrijeme pohrane2017-10-01 16:40:35