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Isolation of F. novicida-Containing Phagosome from Infected Human Monocyte Derived Macrophages
Frontiers in Cellular and Infection Microbiology, 7 (2017); 1-11. https://doi.org/10.3389/fcimb.2017.00303
Marečić, Valentina; Shevchuk, Olga; Ožanič, Mateja; Mihelčić, Mirna; Steinert, Michael; Jurak Begonja, Antonija; Abu Kwaik, Yousef; Šantić, Marina

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Marečić, V., Shevchuk, O., Ožanič, M., Mihelčić, M., Steinert, M., Jurak Begonja, A. ... Šantić, M. (2017). Isolation of F. novicida-Containing Phagosome from Infected Human Monocyte Derived Macrophages. Frontiers in Cellular and Infection Microbiology, 7., 1-11. doi: 10.3389/fcimb.2017.00303

Marečić, Valentina, et al. "Isolation of F. novicida-Containing Phagosome from Infected Human Monocyte Derived Macrophages." Frontiers in Cellular and Infection Microbiology, vol. 7, 2017, str. 1-11. https://doi.org/10.3389/fcimb.2017.00303

Marečić, Valentina, Olga Shevchuk, Mateja Ožanič, Mirna Mihelčić, Michael Steinert, Antonija Jurak Begonja, Yousef Abu Kwaik i Marina Šantić. "Isolation of F. novicida-Containing Phagosome from Infected Human Monocyte Derived Macrophages." Frontiers in Cellular and Infection Microbiology 7 (2017): 1-11. https://doi.org/10.3389/fcimb.2017.00303

Marečić, V., et al. (2017) 'Isolation of F. novicida-Containing Phagosome from Infected Human Monocyte Derived Macrophages', Frontiers in Cellular and Infection Microbiology, 7, str. 1-11. doi: 10.3389/fcimb.2017.00303

Marečić V, Shevchuk O, Ožanič M, Mihelčić M, Steinert M, Jurak Begonja A, i sur.. Isolation of F. novicida-Containing Phagosome from Infected Human Monocyte Derived Macrophages. Frontiers in Cellular and Infection Microbiology [Internet]. 2017. [pristupljeno 20.04.2024.];7:1-11. doi: 10.3389/fcimb.2017.00303

V. Marečić, et al., "Isolation of F. novicida-Containing Phagosome from Infected Human Monocyte Derived Macrophages", Frontiers in Cellular and Infection Microbiology, vol. 7, str. 1-11, 2017. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:589065. [Citirano: 20.04.2024.]

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Naslov (engleski)Isolation of F. novicida-Containing Phagosome from Infected Human Monocyte Derived Macrophages
AutorValentina Marečić
AutorOlga Shevchuk
AutorMateja Ožanič
AutorMirna Mihelčić
AutorMichael Steinert
AutorAntonija Jurak Begonja
AutorYousef Abu Kwaik
AutorMarina Šantić
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za mikrobiologiju i parazitologiju)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Kliničke medicinske znanosti
Medicinska mikrobiologija
Sažetak (engleski)
Francisella is a gram-negative bacterial pathogen, which causes tularemia in humans and animals. A crucial step of Francisella infection is its invasion of macrophage cells. Biogenesis of the Francisella-containing phagosome (FCP) is arrested for ∼15min at the endosomal stage, followed by gradual bacterial escape into the cytosol, where the microbe proliferates. The crucial step in pathogenesis of tularemia is short and transient presence of the bacterium within phagosome. Isolation of FCPs for further studies has been challenging due to the short period of time of bacterial residence in it and the characteristics of the FCP. Here, we will for the first time present the method for isolation of the FCPs from infected human monocytes-derived macrophages (hMDMs). For elimination of lysosomal compartment these organelles were pre-loaded with dextran coated colloidal iron particles prior infection and eliminated by magnetic separation of the post-nuclear supernatant (PNS). We encountered the challenge that mitochondria has similar density to the FCP. To separate the FCP in the PNS from mitochondria, we utilized iodophenylnitrophenyltetrazolium, which is converted by the mitochondrial succinate dehydrogenase into formazan, leading to increased density of the mitochondria and allowing separation by the discontinuous sucrose density gradient ultracentrifugation. The purity of the FCP preparation and its acquisition of early endosomal markers was confirmed by Western blots, confocal and transmission electron microscopy. Our strategy to isolate highly pure FCPs from macrophages should facilitate studies on the FCP and its biogenesis.13
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaFrontiers in Cellular and Infection Microbiology
Brojčani podacivol. 7, str. 1-11
p-ISSN2235-2988
DOIhttps://doi.org/10.3389/fcimb.2017.00303
URN:NBNurn:nbn:hr:184:589065
Datum objave publikacije2017
ProjektŠifra: IP-2016-06-9003
Naziv (hrvatski): Uloga unutarstaničnog života Francisella tularensis u patogenezi eksperimentalne tularemije
Kratica: FRANCYCLE
Voditelj: Marina Šantić
Pravna nadležnost: Hrvatska
Financijer: HRZZ
Linija financiranja: IP
URL dokumentahttp://bib.irb.hr/884138
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Zaštićeno autorskim pravom.
Datum i vrijeme pohrane2017-09-26 19:42:53