Breast cancer is the most common malignancy in women, with hormone receptor-positive and human epidermal growth factor receptor 2-negative (HR+/HER2-) breast cancers representing the vast majority of receptor subtypes. This study aimed to compare two assessment methods of the tumor-infiltrating lymphocytes (TILs) in HR+/HER2- breast cancer and to examine the associations of the TILs density with clinicopathological characteristics and prognostic significance. The analysis included Bioptic material from 37 female patients treated in the Clinical Hospital Center (CHC) Rijeka from 2007 to 2019. The median age was 57 years, with a range of 39 to 75 years. Clinical and pathological data were collected from the computer database of the Department of Pathology and Pathological Anatomy of the Faculty of Medicine, University of Rijeka, and from the WinBis database of CHC Rijeka. The TIL density was evaluated by light microscopic analysis of hematoxylin and eosin (HE) stained specimens; following the International Immuno-Oncology Biomarker Working Group recommendations, and by a four-degree scale previously used in the inflammatory reaction in colorectal cancer research work (Klinturp et al). MedCalc statistical software was used for statistical analysis. Evaluated by both methods, TIL was significantly positively correlated with the presence of lymphovascular invasion (LVI) and was predominantly presented in low-stage disease. Assessed with a four-degree scale, TIL was presented in more significant proportions in positive LVI cases and was negatively correlated with the clinical stage. The intraclass correlation coefficient was calculated to determine the agreement of TIL densities evaluated in both ways. The value of 0.7577 demonstrated good reliability among the methods used.