prikaz prve stranice dokumenta Arf GTPases Are Required for the Establishment of the Pre-Assembly Compartment in the Early Phase of Cytomegalovirus Infection
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Arf GTPases Are Required for the Establishment of the Pre-Assembly Compartment in the Early Phase of Cytomegalovirus Infection
Life (Basel), 11 (2021), 8; 867. https://doi.org/10.3390/life11080867
Pavišić, Valentino; Mahmutefendić Lučin, Hana; Blagojević Zagorac, Gordana; Lučin, Pero

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Pavišić, V., Mahmutefendić Lučin, H., Blagojević Zagorac, G. i Lučin, P. (2021). Arf GTPases Are Required for the Establishment of the Pre-Assembly Compartment in the Early Phase of Cytomegalovirus Infection. Life (Basel), 11. (8). doi: 10.3390/life11080867

Pavišić, Valentino, et al. "Arf GTPases Are Required for the Establishment of the Pre-Assembly Compartment in the Early Phase of Cytomegalovirus Infection." Life (Basel), vol. 11, br. 8, 2021. https://doi.org/10.3390/life11080867

Pavišić, Valentino, Hana Mahmutefendić Lučin, Gordana Blagojević Zagorac i Pero Lučin. "Arf GTPases Are Required for the Establishment of the Pre-Assembly Compartment in the Early Phase of Cytomegalovirus Infection." Life (Basel) 11, br. 8 (2021). https://doi.org/10.3390/life11080867

Pavišić, V., et al. (2021) 'Arf GTPases Are Required for the Establishment of the Pre-Assembly Compartment in the Early Phase of Cytomegalovirus Infection', Life (Basel), 11(8). doi: 10.3390/life11080867

Pavišić V, Mahmutefendić Lučin H, Blagojević Zagorac G, Lučin P. Arf GTPases Are Required for the Establishment of the Pre-Assembly Compartment in the Early Phase of Cytomegalovirus Infection. Life (Basel) [Internet]. 23.08.2021. [pristupljeno 12.11.2024.];11(8). doi: 10.3390/life11080867

V. Pavišić, H. Mahmutefendić Lučin, G. Blagojević Zagorac i P. Lučin, "Arf GTPases Are Required for the Establishment of the Pre-Assembly Compartment in the Early Phase of Cytomegalovirus Infection", Life (Basel), vol. 11, br. 8, Kolovoz 2021. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:236112. [Citirano: 12.11.2024.]

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Naslov (engleski)Arf GTPases Are Required for the Establishment of the Pre-Assembly Compartment in the Early Phase of Cytomegalovirus Infection
AutorValentino Pavišić
AutorHana Mahmutefendić Lučin
AutorGordana Blagojević Zagorac
AutorPero Lučin
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za fiziologiju, imunologiju i patofiziologiju)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Fiziologija čovjeka
Sažetak (engleski)
Shortly after entering the cells, cytomegaloviruses (CMVs) initiate massive reorganization of cellular endocytic and secretory pathways, which results in the forming of the cytoplasmic virion assembly compartment (AC). We have previously shown that the formation of AC in murine CMV- (MCMV) infected cells begins in the early phase of infection (at 4-6 hpi) with the pre-AC establishment. Pre-AC comprises membranes derived from the endosomal recycling compartment, early endosomes, and the trans-Golgi network, which is surrounded by fragmented Golgi cisterns. To explore the importance of Arf GTPases in the biogenesis of the pre-AC, we infected Balb 3T3 cells with MCMV and analyzed the expression and intracellular localization of Arf proteins in the early phases (up to 16 hpi) of infection and the development of pre-AC in cells with a knockdown of Arf protein expression by small interfering RNAs (siRNAs). Herein, we show that even in the early phase, MCMVs cause massive reorganization of the Arf system of the host cells and induce the over-recruitment of Arf proteins onto the membranes of pre-AC. Knockdown of Arf1, Arf3, Arf4, or Arf6 impaired the establishment of pre-AC. However, the knockdown of Arf1 and Arf6 also abolished the establishment of infection. Our study demonstrates that Arf GTPases are required for different steps of early cytomegalovirus infection, including the establishment of the pre-AC.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaLife (Basel)
Brojčani podacivol. 11, br. 8, 867
BPC1800 CHF
e-ISSN0000-0000
DOIhttps://doi.org/10.3390/life11080867
URN:NBNurn:nbn:hr:184:236112
Datum objave elektroničkog izdanja2021-08-23
ProjektŠifra: IP-2020-02-1323
Naziv (hrvatski): Stanični sustav Arf GTPaza u patogenezi citomegalovirusne infekcije
Kratica: Arf & CMV
Voditelj: Gordana Blagojević Zagorac
Pravna nadležnost: Hrvatska
Financijer: HRZZ
URL dokumentahttps://www.mdpi.com/2075-1729/11/8/867
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje-Dijeli pod istim uvjetima 3.0 (CC BY-SA 3.0)
Datum i vrijeme pohrane2022-05-18 06:32:30