prikaz prve stranice dokumenta The Cytomegalovirus Tegument Protein UL35 Antagonizes Pattern Recognition Receptor- Mediated Type I IFN Transcription
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The Cytomegalovirus Tegument Protein UL35 Antagonizes Pattern Recognition Receptor- Mediated Type I IFN Transcription
Microorganisms, 8 (2020), 6; 1-26. https://doi.org/10.3390/microorganisms8060790
Fabits, Markus; Gonçalves Magalhães, Vladimir; Chan, Baca; Girault, Virginie; Elbasani, Endrit; Rossetti, Elisa; Saeland, Eirikur; Messerle, Martin; Pichlmair, Andreas; Lisnić, Vanda Juranić; Brinkmann, Melanie M.

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Fabits, M., Gonçalves Magalhães, V., Chan, B., Girault, V., Elbasani, E., Rossetti, E. ... Brinkmann, M. M. (2020). The Cytomegalovirus Tegument Protein UL35 Antagonizes Pattern Recognition Receptor- Mediated Type I IFN Transcription. Microorganisms, 8. (6), 1-26. doi: 10.3390/microorganisms8060790

Fabits, Markus, et al. "The Cytomegalovirus Tegument Protein UL35 Antagonizes Pattern Recognition Receptor- Mediated Type I IFN Transcription." Microorganisms, vol. 8, br. 6, 2020, str. 1-26. https://doi.org/10.3390/microorganisms8060790

Fabits, Markus, Vladimir Gonçalves Magalhães, Baca Chan, Virginie Girault, Endrit Elbasani, Elisa Rossetti, Eirikur Saeland, et al. "The Cytomegalovirus Tegument Protein UL35 Antagonizes Pattern Recognition Receptor- Mediated Type I IFN Transcription." Microorganisms 8, br. 6 (2020): 1-26. https://doi.org/10.3390/microorganisms8060790

Fabits, M., et al. (2020) 'The Cytomegalovirus Tegument Protein UL35 Antagonizes Pattern Recognition Receptor- Mediated Type I IFN Transcription', Microorganisms, 8(6), str. 1-26. doi: 10.3390/microorganisms8060790

Fabits M, Gonçalves Magalhães V, Chan B, Girault V, Elbasani E, Rossetti E, i sur.. The Cytomegalovirus Tegument Protein UL35 Antagonizes Pattern Recognition Receptor- Mediated Type I IFN Transcription. Microorganisms [Internet]. 2020. [pristupljeno 12.11.2024.];8(6):1-26. doi: 10.3390/microorganisms8060790

M. Fabits, et al., "The Cytomegalovirus Tegument Protein UL35 Antagonizes Pattern Recognition Receptor- Mediated Type I IFN Transcription", Microorganisms, vol. 8, br. 6, str. 1-26, 2020. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:809461. [Citirano: 12.11.2024.]

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Naslov (engleski)The Cytomegalovirus Tegument Protein UL35 Antagonizes Pattern Recognition Receptor- Mediated Type I IFN Transcription
AutorMarkus Fabits
AutorVladimir Gonçalves Magalhães
AutorBaca Chan
AutorVirginie Girault
AutorEndrit Elbasani
AutorElisa Rossetti
AutorEirikur Saeland
AutorMartin Messerle
AutorAndreas Pichlmair
AutorVanda Juranić Lisnić
AutorMelanie M. Brinkmann
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za histologiju i embriologiju)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Sažetak (engleski)
Abstract: The rapid activation of pattern recognition receptor (PRR)-mediated type I interferon (IFN) signaling is crucial for the host response to infection. In turn, human cytomegalovirus (HCMV) must evade this potent response to establish life- long infection. Here, we reveal that the HCMV tegument protein UL35 antagonizes the activation of type I IFN transcription downstream of the DNA and RNA sensors cGAS and RIG-I, respectively. We show that ectopic expression of UL35 diminishes the type I IFN response, while infection with a recombinant HCMV lacking UL35 induces an elevated type I IFN response compared to wildtype HCMV. With a series of luciferase reporter assays and the analysis of signaling kinetics upon HCMV infection, we observed that UL35 downmodulates PRR signaling at the level of the key signaling factor TANK- binding kinase 1 (TBK1). Finally, we demonstrate that UL35 and TBK1 co-immunoprecipitate when co-expressed in HEK293T cells. In addition, we show that a previously reported cellular binding partner of UL35, O-GlcNAc transferase (OGT), post-translationally GlcNAcylates UL35, but that this modification is not required for the antagonizing effect of UL35 on PRR signaling. In summary, we have identified UL35 as the first HCMV protein to antagonize the type I IFN response at the level of TBK1, thereby enriching our understanding of how this important herpesvirus escapes host immune responses.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaMicroorganisms
Brojčani podacivol. 8, br. 6, str. 1-26
p-ISSN2076-2607
DOIhttps://doi.org/10.3390/microorganisms8060790
URN:NBNurn:nbn:hr:184:809461
Datum objave publikacije2020
URL dokumentahttp://bib.irb.hr/1107363
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje-Nekomercijalno 4.0 međunarodna (CC BY-NC 4.0)
Datum i vrijeme pohrane2022-03-10 14:34:02