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Epidermal Growth Factor Receptor Protein Expression and Gene Amplification in Normal, Hyperplastic, and Cancerous Glottic Tissue: Immunohistochemical and Fluorescent in Situ Hybridization Study on Tissue Microarrays
Croatian Medical Journal, 50 (2009), 4; 370-379. urn:nbn:hr:184:948453
Braut, Tamara; Krstulja, Mira; Kujundžić, Milodar; Manestar, Dubravko; Hadžisejdić, Ita; Jonjić, Nives; Grahovac, Blaženka; Manestar, Darko

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Braut, T., Krstulja, M., Kujundžić, M., Manestar, D., Hadžisejdić, I., Jonjić, N. ... Manestar, D. (2009). Epidermal Growth Factor Receptor Protein Expression and Gene Amplification in Normal, Hyperplastic, and Cancerous Glottic Tissue: Immunohistochemical and Fluorescent in Situ Hybridization Study on Tissue Microarrays. Croatian Medical Journal, 50. (4), 370-379. Preuzeto s https://urn.nsk.hr/urn:nbn:hr:184:948453

Braut, Tamara, et al. "Epidermal Growth Factor Receptor Protein Expression and Gene Amplification in Normal, Hyperplastic, and Cancerous Glottic Tissue: Immunohistochemical and Fluorescent in Situ Hybridization Study on Tissue Microarrays." Croatian Medical Journal, vol. 50, br. 4, 2009, str. 370-379. https://urn.nsk.hr/urn:nbn:hr:184:948453

Braut, Tamara, Mira Krstulja, Milodar Kujundžić, Dubravko Manestar, Ita Hadžisejdić, Nives Jonjić, Blaženka Grahovac i Darko Manestar. "Epidermal Growth Factor Receptor Protein Expression and Gene Amplification in Normal, Hyperplastic, and Cancerous Glottic Tissue: Immunohistochemical and Fluorescent in Situ Hybridization Study on Tissue Microarrays." Croatian Medical Journal 50, br. 4 (2009): 370-379. https://urn.nsk.hr/urn:nbn:hr:184:948453

Braut, T., et al. (2009) 'Epidermal Growth Factor Receptor Protein Expression and Gene Amplification in Normal, Hyperplastic, and Cancerous Glottic Tissue: Immunohistochemical and Fluorescent in Situ Hybridization Study on Tissue Microarrays', Croatian Medical Journal, 50(4), str. 370-379. Preuzeto s: https://urn.nsk.hr/urn:nbn:hr:184:948453 (Datum pristupa: 13.11.2024.)

Braut T, Krstulja M, Kujundžić M, Manestar D, Hadžisejdić I, Jonjić N, i sur.. Epidermal Growth Factor Receptor Protein Expression and Gene Amplification in Normal, Hyperplastic, and Cancerous Glottic Tissue: Immunohistochemical and Fluorescent in Situ Hybridization Study on Tissue Microarrays. Croatian Medical Journal [Internet]. 2009. [pristupljeno 13.11.2024.];50(4):370-379. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:948453

T. Braut, et al., "Epidermal Growth Factor Receptor Protein Expression and Gene Amplification in Normal, Hyperplastic, and Cancerous Glottic Tissue: Immunohistochemical and Fluorescent in Situ Hybridization Study on Tissue Microarrays", Croatian Medical Journal, vol. 50, br. 4, str. 370-379, 2009. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:948453. [Citirano: 13.11.2024.]

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Podaci o radu
Naslov (engleski)Epidermal Growth Factor Receptor Protein Expression and Gene Amplification in Normal, Hyperplastic, and Cancerous Glottic Tissue: Immunohistochemical and Fluorescent in Situ Hybridization Study on Tissue Microarrays
AutorTamara Braut
AutorMira Krstulja
AutorMilodar Kujundžić
AutorDubravko Manestar
AutorIta Hadžisejdić
AutorNives Jonjić
AutorBlaženka Grahovac
AutorDarko Manestar
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za opću patologiju i patološku anatomiju)
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za otorinolaringologiju)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Kliničke medicinske znanosti
Otorinolaringologija
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Kliničke medicinske znanosti
Patologija
Sažetak (engleski)
Aim To evaluate the importance of epidermal growth factor receptor (EGFR) protein overexpression and gene amplification in carcinogenesis of glottic cancer. Method In order to evaluate EGFR expression at protein and gene level, immunohistochemical (IHC) analysis and fluorescent in situ hybridization (FISH) were performed on tissue microarrays of laryngeal tissue (145 samples) – 38 samples of normal mucosa, 46 samples of hyperplastic lesions, and 61 samples of cancerous lesions. Results Membranous (mEGFR) and cytoplasmic (cEGFR) EGFR expression was significantly different between the analyzed groups. The differences were most striking in the suprabasal-transforming zone. IHC evaluation showed that high and low mEGFR staining contributed to the differentiation of dysplastic lesions, simple hyperplasia, and cancerous tissue, as well as between different degrees of atypia in hyperplastic lesions (P < 0.050). EGFR gene amplification was not found in simple and abnormal hyperplastic lesions, but it was confirmed in 2/21 atypical hyperplasias, indicating that gene amplification can facilitate identification of malignant potential in hyperplastic lesions. In cancerous tissue, EGFR gene amplification was found in 8/50 samples. EGFR gene amplification was found in preinvasive cancer in one patient. In invasive carcinomas, gene amplification was not associated with stage or grade. Carcinomas with gene amplification showed significantly higher cEGFR expression (basal layer P = 0.003; suprabasal layer P = 0.002). Conclusions This study confirmed an increase in EGFR protein expression and gene amplification with the increase in biological aggressiveness of glottic lesions. A correlation between EGFR gene amplification and protein expression was established. Gene amplification proved to be an early event in glottic carcinogenesis, indicating its importance for glottic cancer prevention, early detection, and protocol selection.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaCroatian Medical Journal
Brojčani podacivol. 50, br. 4, str. 370-379
p-ISSN0353-9504
e-ISSN1332-8166
URN:NBNurn:nbn:hr:184:948453
Datum objave publikacije2009
URL dokumentahttps://hrcak.srce.hr/47857
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 4.0 međunarodna (CC BY 4.0)
Datum i vrijeme pohrane2022-01-26 10:23:12